Novel injectable adhesive hydrogel loaded with exosomes for holistic repair of hemophilic articular cartilage defect

Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 85 - 111

Published: Aug. 29, 2024

Hemophilic articular cartilage damage presents a significant challenge for surgeons, characterized by recurrent intraarticular bleeding, severe inflammatory microenvironment, and limited self-repair capability of tissue. Currently, there is lack tissue engineering-based integrated therapies that address both early hemostasis, anti-inflammation, long-lasting chondrogenesis hemophilic defects. Herein, we developed an adhesive hydrogel using oxidized chondroitin sulfate gelatin, loaded with exosomes derived from bone marrow stem cells (BMSCs) (Hydrogel-Exos). This demonstrated favorable injectability, self-healing, biocompatibility, biodegradability, swelling, frictional mechanical properties, providing comprehensive approach to treating The hydrogel, featuring dynamic Schiff base bonds hydrogen bonds, exhibited excellent wet adhesiveness hemostatic properties. In pig model, the could be smoothly injected into knee joint defect site gelled

Language: Английский

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Gradient scaffolds for osteochondral tissue engineering and regeneration

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 498, P. 154797 - 154797

Published: Aug. 13, 2024

Language: Английский

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Modern Approach to Testing the Biocompatibility of Osteochondral Scaffolds in Accordance with the 3Rs Principle─Preclinical In Vitro, Ex Vivo, and In Vivo Studies Using the Biphasic Curdlan-Based Biomaterial

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

The aim of this work is to provide a comprehensive set biological tests assess the biomedical potential novel osteochondral scaffolds with methods proposed comply 3Rs principle, focusing here on biphasic Curdlan-based scaffold as promising model biomaterial. In vitro experiments include evaluation cytotoxicity, mutagenicity, and genotoxicity referring ISO standards, assessment viability proliferation human chondrocytes osteoblasts, estimation inflammation after direct contact biomaterials macrophages. Ex vivo assessments response surrounding tissue incubation implanted involve an toxicity regenerative biomaterial in zebrafish (larvae adults) defects dogs (veterinary patients). applied allows us show that does not induce cytotoxicity (cell close 100%), mutagenicity (the level reversion 2× higher compared control), (it exhibit any change chromosomal aberration; frequency micronuclei, micronucleated binucleated cells, cytokinesis-block index comparable control; moreover, it cause formation comets cells). This also promotes osteoblasts OD values between fourth seventh day increase by approximately 1.6×). stimulates only transient inflammatory ex vivo. Danio rerio larvae malformation enables proper regeneration caudal fin adults. Finally, supports defect veterinary patients. Thus, proposal use alternative for opposed commonly used using large numbers laboratory animals.

Language: Английский

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Smart hydrogel: A new platform for cancer therapy

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 340, P. 103470 - 103470

Published: March 7, 2025

Language: Английский

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Current Status of Research on Biomimetic Hydrogels for Articular Cartilage

Journal of Bionic Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Language: Английский

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Productizing Nano-Bioactive Glass-Based Bilayer Scaffolds: A Graft for Reconstruction of Mandibular and Femoral Bone Defects

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(19), P. 25317 - 25332

Published: May 6, 2024

This investigation aimed to construct a bilayer scaffold integrating alginate and gelatin with nanobioactive glass (BG), recognized for their efficacy in tissue regeneration drug delivery. Scaffolds, namely, alginate/gelatin (AG), alginate-/actonel (AGD), actenol/gelatin-45S5 BG (4AGD), alginate-actonel/gelatin-59S (5AGD), were assembled using cost-effective freeze-drying method, followed by detailed structural via powder X-ray diffraction as well morphological characterization field emission scanning electron microscopy (FESEM). FESEM revealed honeycomb-like morphology distinct pore sizes nutrient, oxygen, transport. The scaffolds evidently exhibited hemocompatibility, high porosity, good swelling capacity, biodegradability. In vitro studies demonstrated sustained release, particularly containing actonel. vivo tests showed that the promoted new bone formation, surpassing control group area increase. interaction of collagen released ions improved osteoblastic function volume fraction. findings suggest this could be beneficial treating critical-sized defects, especially mandibular femoral regions.

Language: Английский

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Ag-Doped SnSe Nanosheets for Enhanced NIR-II Light-Induced Apoptosis

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

Triple-negative breast cancer (TNBC) is one of the most aggressive and challenging subtypes with limited treatment options poor prognosis. To overcome these obstacles, Ag-doped SnSe (ASS) nanosheets were used as photonic hyperthermia agents for treating TNBC. ASS featured a high photothermal-conversion efficiency photon effect. Additionally, biosafety assessments, including hemolysis assays histopathological analysis, confirmed that was biosafety. These findings suggest ASS, in synergy second near-infrared biowindow (NIR-II) light irradiation, offers noninvasive, effective, safe therapeutic strategy This innovative approach exemplifies evolving landscape TNBC treatment, where technology biology converge to offer more precise personalized therapies.

Language: Английский

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