Rational design of DAHP synthase and prephenate dehydrogenase for metabolic engineering of Bacillus amyloliquefaciens to produce L-tyrosine

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 307, P. 142076 - 142076

Published: March 15, 2025

Language: Английский

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Functional analysis of tandem glycosyltransferases catalyzing the O-glycosylation of flavonoid 4'-O-glycosyl-6-C-glycosides biosynthesis from Isatis indigotica

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109920 - 109920

Published: April 1, 2025

Language: Английский

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Advances and Challenges in Biomanufacturing of Glycosylation of Natural Products

Fermentation, Journal Year: 2024, Volume and Issue: 10(7), P. 349 - 349

Published: July 9, 2024

Glycosylation is one of the most common and important modifications in natural products (NPs), which can alter biological activities properties NPs, effectively increase structural diversity, improve pharmacological activities. The biosynthesis glycosylation involves multiple complex processes, are coordinated by many enzymes. UDP-glycosyltransferases (UGTs) play a crucial role modification, have attracted long-term widespread research attention. UGTs catalyze O-, C-, S-, N-glycosylation different substrates, producing variety glycosides with broad activity, while improving solubility, stability, bioavailability, other functions NPs. In recent years, rapid development synthetic biology advanced manufacturing technologies, especially application artificial intelligence field biology, has led to series new discoveries NP UGT. This work summarizes latest progress challenges glycosylation, covering results potential applications glycosylated derivatives terpenes, flavonoids, polyphenols, aromatic compounds, compounds terms biogenesis. Looking future, may leverage intelligence-driven techniques decipher genes related pathway, expected further promote large-scale synthesis diversity NPs pharmaceutical, functional food, cosmetic industries.

Language: Английский

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Insights into functional divergence, catalytic versatility and specificity of small molecule glycosyltransferases

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138821 - 138821

Published: Dec. 1, 2024

Language: Английский

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Multi-strategy integration improves 28-O-glucosylation of asiatic acid into asiatic acid monoglucoside

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104832 - 104832

Published: July 27, 2024

Language: Английский

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Mining and Engineering the Di-O-glycosylation Pattern of UGT72B1 for the Highly Efficient O-Glycosylation of Endogenous Quercetin

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(45), P. 25219 - 25228

Published: Oct. 30, 2024

Compared with mono-O-glycosylation, di-O-glycosylation endows the precursor better performance. However, mining and engineering of patterns glycosyltransferases are limited, hindering their synthetic applications. Here, an Arabidopsis xenobiotic-transforming glycosyltransferase, UGT72B1, was found to catalyze glycosylation endogenous quercetin its monoglycosides, generating di-O-glucosides. Mutating M17/G18/Y315 into L/T/Q in UGT72B1 altered regioselectivity toward 7-O-glucoside, enzymatically another 3,7-di-O-glycoside up a 100% conversion rate, increased sugar donor preference. Altering regiospecificity likely required coordination between entrance active site, where orientations acceptors donors shift adopt lower binding energy state. Moreover, 3,4′-di-O-β-d-glucoside 3,7-di-O-β-d-glucoside synthesized were have highest anti-inflammatory activities. Overall, this work presents efficient strategy engineer for synthesis di-O-β-d-glucosides be used as food additives, therapeutics, nutraceuticals.

Language: Английский

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Engineering Escherichia coli for Efficient De Novo Synthesis of Salidroside

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Salidroside is a high-value plant-derived glycoside with diverse biological activities, but the main industrial salidroside production method, extraction from

Language: Английский

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