A general method to optimize and functionalize red-shifted rhodamine dyes

Nature Methods, Journal Year: 2020, Volume and Issue: 17(8), P. 815 - 821

Published: July 27, 2020

Language: Английский

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Caveat fluorophore: an insiders’ guide to small-molecule fluorescent labels

Nature Methods, Journal Year: 2021, Volume and Issue: 19(2), P. 149 - 158

Published: Dec. 23, 2021

Language: Английский

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Rational design of small molecule fluorescent probes for biological applications

Organic & Biomolecular Chemistry, Journal Year: 2020, Volume and Issue: 18(30), P. 5747 - 5763

Published: Jan. 1, 2020

Guidelines based on photophysical tuning, reactivity, isomerization, and energy transfer for rational design of synthetic fluorescent probes biological systems.

Language: Английский

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Super-resolution Microscopy with Single Molecules in Biology and Beyond–Essentials, Current Trends, and Future Challenges

Journal of the American Chemical Society, Journal Year: 2020, Volume and Issue: 142(42), P. 17828 - 17844

Published: Oct. 9, 2020

Single-molecule super-resolution microscopy has developed from a specialized technique into one of the most versatile and powerful imaging methods nanoscale over past two decades. In this perspective, we provide brief overview historical development field, fundamental concepts, methodology required to obtain maximum quantitative information, current state art. Then, will discuss emerging perspectives areas where innovation further improvement are needed. Despite tremendous progress, full potential single-molecule is yet be realized, which enabled by research ahead us.

Language: Английский

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Fluorescent amino acids as versatile building blocks for chemical biology

Nature Reviews Chemistry, Journal Year: 2020, Volume and Issue: 4(6), P. 275 - 290

Published: May 13, 2020

Language: Английский

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The HaloTag as a general scaffold for far-red tunable chemigenetic indicators

Nature Chemical Biology, Journal Year: 2021, Volume and Issue: 17(6), P. 718 - 723

Published: April 1, 2021

Language: Английский

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Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(36), P. 14592 - 14600

Published: Aug. 30, 2021

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist a dynamic equilibrium between fluorescent zwitterion and nonfluorescent but cell-permeable spirocyclic form. Different imaging require different positions this equilibrium, an adjustment poses challenge design suitable probes. We describe here how conversion ortho-carboxy moiety given rhodamine into substituted acyl benzenesulfonamides alkylamides permits systematic tuning spirocyclization with unprecedented accuracy over large range. allows one to transform same either highly fluorogenic probe live-cell-stimulated emission depletion (STED) microscopy or spontaneously blinking dye single-molecule localization (SMLM). used approach generate differently colored probes optimized labeling systems applications.

Language: Английский

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Engineered HaloTag variants for fluorescence lifetime multiplexing

Nature Methods, Journal Year: 2021, Volume and Issue: 19(1), P. 65 - 70

Published: Dec. 16, 2021

Abstract Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce variants either increased or decreased brightness and lifetime compared HaloTag7 when labeled rhodamines. Combining these enabled live-cell multiplexing of three cellular targets one spectral channel using a single fluorophore the generation lifetime-based biosensor. Additionally, brightest variant showed up to 40% higher imaging applications.

Language: Английский

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A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility

Cell, Journal Year: 2022, Volume and Issue: 185(18), P. 3390 - 3407.e18

Published: Sept. 1, 2022

Chemical synapses between axons and dendrites mediate neuronal intercellular communication. Here, we describe a synapse primary cilia: the axo-ciliary synapse. Using enhanced focused ion beam-scanning electron microscopy on samples with optimally preserved ultrastructure, discovered brainstem serotonergic cilia of hippocampal CA1 pyramidal neurons. Functionally, these are enriched in ciliary-restricted serotonin receptor, 5-hydroxytryptamine receptor 6 (5-HTR6). cilia-targeted sensor, show that opto- chemogenetic stimulation releases onto cilia. Ciliary 5-HTR6 activates non-canonical G

Language: Английский

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A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: April 27, 2022

Abstract The quality and application of super-resolution fluorescence imaging greatly lie in the dyes’ properties, including photostability, brightness, Stokes shift. Here we report a synergistic strategy to simultaneously improve such properties regular fluorophores. Introduction quinoxaline motif with fine-tuned electron density conventional rhodamines generates new dyes vibration structure inhibited twisted-intramolecular-charge-transfer (TICT) formation synchronously, thus increasing brightness photostability while enlarging fluorophore YL578 exhibits around twofold greater shift than its parental fluorophore, Rhodamine B. Importantly, Stimulated Emission Depletion (STED) microscopy, derived probe possesses superior renders threefold more frames carbopyronine based probes (CPY-Halo 580CP-Halo), known as photostable fluorophores for STED imaging. Furthermore, is well generalized offer class bright fluorescent long (up 136 nm) bioimaging biosensing.

Language: Английский

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