Short-chain fatty acids abrogate Japanese encephalitis virus-induced inflammation in microglial cells via miR-200a-3p/ZBTB20/IKβα axis DOI Creative Commons

Atreye Majumdar,

Indira Priya Siva Venkatesh,

Vivek Swarup

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(7)

Published: June 13, 2024

Japanese encephalitis virus (JEV), a member of the Flaviviridae family, is leading cause viral in humans. Survivors this infection often develop lifelong neurological sequelae. Short-chain fatty acids (SCFAs) produced gut are vital mediators gut-brain axis. We aimed to study microRNA-based mechanisms SCFAs an vitro model JEV infection. N9 microglial cells were pretreated with SCFA cocktail before Cytokine bead analysis, immunoblotting, and PCR performed analyze relevant inflammatory markers. microRNA sequencing was using Illumina Hiseq, bioinformatics tools used for differentially expressed (DE) miRNAs weighted gene co-expression network analysis (WGCNA). mimic/inhibitor experiments luciferase assay miRNA-target interaction. A significant reduction monocyte chemoattractant protein (MCP1) tumor necrosis factor alpha (TNFα) along reduced expression phospho-nuclear kappa B (phospho-NF-κB) observed conditions. Significant attenuation histone deacetylase activity recorded. miRNA revealed 160 DE + JEV-treated at 6 h post-infection. WGCNA miR-200a-3p, hub significantly upregulated Transcription ZBTB20 bioinformatically predicted validated as target miR-200a-3p. Further demonstrated that miR-200-3p regulated Iκβα possibly dampened NF-κB signal activation downstream. The axis plays pivotal role physiological state organism. Gut microbiota-derived metabolites known play brain disorders including neuroviral infections. appear quench markers virus-infected vitro. Mechanistically, we demonstrate interaction between miR-200a-3p regulating canonical nuclear (NF-κB) signaling pathway via transcriptional regulation Iκβα. Findings pave way better understanding can be strategies against neuroinflammation.

Language: Английский

The role of short-chain fatty acid in metabolic syndrome and its complications: focusing on immunity and inflammation DOI Creative Commons
Wenqian Yu,

Siyuan Sun,

Qiang Fu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 7, 2025

Metabolic syndrome (Mets) is an important contributor to morbidity and mortality in cardiovascular, liver, neurological, reproductive diseases. Short-chain fatty acid (SCFA), organismal energy donor, has recently been demonstrated increasing number of studies be molecule ameliorating immuno-inflammation, causative factor Mets, improve lipid distribution, blood glucose, body weight levels animal models Mets. This study reviews recent research advances on SCFA Mets from immune-inflammatory perspective, including complications dominated by chronic inflammation, as well the fact that these findings also contribute understanding specific mechanisms which gut flora metabolites metabolic processes humans. review proposes emerging role for inflammatory followed identification major ambiguities further understand anti-inflammatory potential this substance In addition, novel strategies modulate treatment may help mitigate prognosis its complications.

Language: Английский

Citations

3
The Gut-Brain Axis and Neuroinflammation in Traumatic Brain Injury DOI
Mega O. Oyovwi, Onoriode Andrew Udi

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 62(4), P. 4576 - 4590

Published: Oct. 28, 2024

Language: Английский

Citations

5
Anticancer Potential of Prebiotics: Targeting Estrogen Receptors and PI3K/AKT/mTOR in Breast Cancer DOI Creative Commons
Hussein Sabit,

Sama Abouelnour,

Bassel M. Hassen

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 990 - 990

Published: April 18, 2025

Estrogen receptors (ERs) play a critical role in breast cancer (BC) development and progression, with ERα being oncogenic ERβ exhibiting tumor-suppressive properties. The interaction between ER signaling other molecular pathways, such as PI3K/AKT/mTOR, influences tumor growth endocrine resistance. Emerging research highlights the of prebiotics modulating gut microbiota, which may influence estrogen metabolism, immune function, therapeutic responses BC. This review explores impact on receptor modulation, microbiota composition, regulation, metabolic pathways cancer. potential adjunctive therapies to enhance treatment efficacy mitigate chemotherapy-related side effects is discussed. A comprehensive analysis recent preclinical clinical studies was conducted, examining reprogramming short-chain fatty acids (SCFAs) derived from prebiotic fermentation epigenetic regulation resistance also evaluated. Prebiotics were found modulate microbiota-estrogen axis, reduce inflammation, responses. SCFAs demonstrated selective downregulation reprogramming, suppressing growth. Synbiotic interventions effects, improving quality life patients. offer promising avenue for prevention therapy by pathways. Future trials are needed validate their treatments management.

Language: Английский

Citations

0
The Role of Microbial Metabolites in the Progression of Neurodegenerative Diseases—Therapeutic Approaches: A Comprehensive Review DOI Open Access

Jorge Missiego-Beltrán,

Ana Isabel Beltrán-Velasco

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10041 - 10041

Published: Sept. 18, 2024

The objective of this review is to provide a comprehensive examination the role microbial metabolites in progression neurodegenerative diseases, as well investigate potential therapeutic interventions targeting microbiota. A literature search was conducted across following databases: PubMed, Scopus, Web Science, ScienceDirect, and Wiley. Key terms related gut microbiota, metabolites, specific metabolic products were used. included both preclinical clinical research articles published between 2000 2024. Short-chain fatty acids have been demonstrated play crucial modulating neuroinflammation, preserving integrity blood–brain barrier, influencing neuronal plasticity protection. Furthermore, amino their derivatives exert significant influence on CNS function. These impact health by regulating intestinal permeability, immune responses, directly neuroinflammation oxidative stress, which are integral diseases. Therapeutic strategies, including prebiotics, probiotics, dietary modifications, fecal microbiota transplantation confirmed restore balance enhance production neuroprotective metabolites. novel drug developments based present promising avenues. its represent field with advance our understanding develop treatments for

Language: Английский

Citations

3
Short-chain fatty acids abrogate Japanese encephalitis virus-induced inflammation in microglial cells via miR-200a-3p/ZBTB20/IKβα axis DOI Creative Commons

Atreye Majumdar,

Indira Priya Siva Venkatesh,

Vivek Swarup

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(7)

Published: June 13, 2024

Japanese encephalitis virus (JEV), a member of the Flaviviridae family, is leading cause viral in humans. Survivors this infection often develop lifelong neurological sequelae. Short-chain fatty acids (SCFAs) produced gut are vital mediators gut-brain axis. We aimed to study microRNA-based mechanisms SCFAs an vitro model JEV infection. N9 microglial cells were pretreated with SCFA cocktail before Cytokine bead analysis, immunoblotting, and PCR performed analyze relevant inflammatory markers. microRNA sequencing was using Illumina Hiseq, bioinformatics tools used for differentially expressed (DE) miRNAs weighted gene co-expression network analysis (WGCNA). mimic/inhibitor experiments luciferase assay miRNA-target interaction. A significant reduction monocyte chemoattractant protein (MCP1) tumor necrosis factor alpha (TNFα) along reduced expression phospho-nuclear kappa B (phospho-NF-κB) observed conditions. Significant attenuation histone deacetylase activity recorded. miRNA revealed 160 DE + JEV-treated at 6 h post-infection. WGCNA miR-200a-3p, hub significantly upregulated Transcription ZBTB20 bioinformatically predicted validated as target miR-200a-3p. Further demonstrated that miR-200-3p regulated Iκβα possibly dampened NF-κB signal activation downstream. The axis plays pivotal role physiological state organism. Gut microbiota-derived metabolites known play brain disorders including neuroviral infections. appear quench markers virus-infected vitro. Mechanistically, we demonstrate interaction between miR-200a-3p regulating canonical nuclear (NF-κB) signaling pathway via transcriptional regulation Iκβα. Findings pave way better understanding can be strategies against neuroinflammation.

Language: Английский

Citations

2