Water Research,
Journal Year:
2023,
Volume and Issue:
244, P. 120469 - 120469
Published: Aug. 8, 2023
Wastewater-based
surveillance
(WBS)
has
been
established
as
a
powerful
tool
that
can
guide
health
policy
at
multiple
levels
of
government.
However,
this
approach
not
well
assessed
more
granular
scales,
including
large
work
sites
such
University
campuses.
Between
August
2021
and
April
2022,
we
explored
the
occurrence
SARS-CoV-2
RNA
in
wastewater
using
qPCR
assays
from
complimentary
sewer
catchments
residential
buildings
spanning
Calgary's
campus
how
compared
to
municipal
treatment
plant
servicing
campus.
Real-time
contact
tracing
data
was
used
evaluate
an
association
between
burden
clinically
confirmed
cases
assess
potential
WBS
for
disease
monitoring
across
worksites.
Concentrations
N1
N2
varied
significantly
six
sampling
-
regardless
several
normalization
strategies
with
certain
consistently
demonstrating
values
1-2
orders
higher
than
others.
Relative
clinical
identified
specific
sewersheds,
provided
one-week
leading
indicator.
Additionally,
our
comprehensive
strategy
enabled
estimation
total
per
capita,
which
lower
surrounding
community
(p≤0.001).
Allele-specific
variants
were
representative
large,
no
time
did
emerging
first
debut
on
This
study
demonstrates
be
efficiently
applied
locate
hotspots
activity
very
scale,
predict
complex
Environment International,
Journal Year:
2022,
Volume and Issue:
171, P. 107718 - 107718
Published: Dec. 23, 2022
SARS-CoV-2
wastewater-based
surveillance
(WBS)
offers
a
tool
for
cost-effective
oversight
of
population's
infections.
In
the
past
two
years,
WBS
has
proven
to
be
crucial
managing
pandemic
across
different
geographical
regions.
However,
changing
context
due
high
levels
COVID-19
vaccination
warrants
closer
examination
its
implication
towards
WBS.
Two
main
questions
were
raised:
1)
Does
cause
shedding
viral
signatures
without
infection?
2)
affect
relationship
between
wastewater
and
clinical
data?
To
answer,
we
review
historical
reports
from
vaccines
in
use
prior
including
polio,
rotavirus,
influenza
measles
infection
provide
perspective
on
implications
strategies
with
regard
potential
into
sewershed.
Additionally,
reviewed
studies
that
looked
data
how
campaigns
could
have
affected
relationship.
Finally,
analyzing
Netherlands,
observed
changes
concomitant
increasing
coverage
switches
dominant
variants
concern.
First,
no
vaccine-derived
is
expected
current
commercial
pipeline
may
confound
interpretation
data.
Secondly,
breakthrough
infections
vaccinated
individuals
contribute
significantly
signals
must
interpreted
light
dynamics
new
The Science of The Total Environment,
Journal Year:
2022,
Volume and Issue:
860, P. 160317 - 160317
Published: Nov. 25, 2022
Wastewater-based
epidemiology
(WBE)
complements
the
clinical
surveillance
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
and
its
variants'
distribution
in
populations.
Many
developed
nations
have
established
national
regional
WBE
systems;
however,
governance
budget
constraints
could
be
obstacles
for
low-
middle-income
countries.
An
urgent
need
thus
exists
to
identify
hotspots
serve
as
sentinel
sites
WBE.
We
hypothesized
that
representative
wastewater
treatment
plants
(WWTPs)
two
international
gateway
cities,
Bangkok
Phuket,
Thailand,
sentineled
SARS-CoV-2
variants
reflect
patterns
at
city
level
early
indicators
new
entering
country.
Municipal
samples
(n
=
132)
were
collected
from
eight
municipal
WWTPs
Phuket
during
19
sampling
events
October
2021
March
2022,
which
tested
by
reverse
transcription-quantitative
polymerase
chain
reaction
(RT-qPCR)
using
US
CDC
N1
N2
multiplex
variant
(Alpha,
Delta,
Omicron
BA.1
BA.2)
singleplex
assays.
The
detection
ratios
followed
similar
trends
testing
data,
each
variant's
viral
loads
agreed
with
daily
cases
(3-d
moving
average).
was
detected
prior
Bangkok,
possibly
due
Phuket's
serving
tourist
communities.
found
BA.2
predominantly
drove
resurgence.
also
noted
a
shifting
pattern
22-d
warning
near
real-time
late
2021.
potential
application
indicate
arrival
re-emerging
or
unprecedented
infectious
agents
support
tourism-dependent
economies
complementing
reduced
regulations
while
maintaining
public
health
protection
via
surveillance.
Water Research,
Journal Year:
2023,
Volume and Issue:
244, P. 120469 - 120469
Published: Aug. 8, 2023
Wastewater-based
surveillance
(WBS)
has
been
established
as
a
powerful
tool
that
can
guide
health
policy
at
multiple
levels
of
government.
However,
this
approach
not
well
assessed
more
granular
scales,
including
large
work
sites
such
University
campuses.
Between
August
2021
and
April
2022,
we
explored
the
occurrence
SARS-CoV-2
RNA
in
wastewater
using
qPCR
assays
from
complimentary
sewer
catchments
residential
buildings
spanning
Calgary's
campus
how
compared
to
municipal
treatment
plant
servicing
campus.
Real-time
contact
tracing
data
was
used
evaluate
an
association
between
burden
clinically
confirmed
cases
assess
potential
WBS
for
disease
monitoring
across
worksites.
Concentrations
N1
N2
varied
significantly
six
sampling
-
regardless
several
normalization
strategies
with
certain
consistently
demonstrating
values
1-2
orders
higher
than
others.
Relative
clinical
identified
specific
sewersheds,
provided
one-week
leading
indicator.
Additionally,
our
comprehensive
strategy
enabled
estimation
total
per
capita,
which
lower
surrounding
community
(p≤0.001).
Allele-specific
variants
were
representative
large,
no
time
did
emerging
first
debut
on
This
study
demonstrates
be
efficiently
applied
locate
hotspots
activity
very
scale,
predict
complex
