Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(23), P. 8362 - 8369
Published: June 2, 2021
Lateral
flow
immunoassay
(LFIA)
has
emerged
as
an
effective
technique
in
the
field
of
food
safety
and
environmental
monitoring.
However,
sensitive
quantitative
detection
is
still
challenging
for
LFIAs
complex
environments.
In
this
work,
a
dual-model
colorimetric/SERS
lateral
ultrasensitive
determination
clenbuterol
was
constructed
based
on
metallic
core-shell
Au/Au
nanostar
acting
multifunction
tag.
Raman
reporter
molecules
are
located
between
core
(AuNP)
shell
(Au
nanostar)
to
form
sandwich
structure,
which
contributes
eliminate
interference
improve
stability.
addition,
provides
much
higher
enhancement
due
presence
sharp
tips
larger
surface
roughness
comparison
with
gold
nanoparticles
(AuNPs).
Thus,
basis
antibody-antigen
interaction,
can
produce
strong
colorimetric
surface-enhanced
spectroscopy
(SERS)
signals
highly
target
analyte,
clenbuterol.
Under
optimal
conditions,
could
be
detected
by
model
visual
limit
5
ng/mL.
Meanwhile,
SERS
signal
accumulated
test
line
low
0.05
ng/mL,
at
least
200-fold
lower
than
that
traditional
AuNPs-based
immunoassay.
Furthermore,
recovery
rates
proposed
method
samples
were
86-110%.
This
tool
antibiotic
residues
analysis
demonstrates
broad
potential
future
applications
Chemical Society Reviews,
Journal Year:
2021,
Volume and Issue:
50(16), P. 8954 - 8994
Published: Jan. 1, 2021
This
article
provides
a
comprehensive
review
of
biosensing
with
DNAzymes,
providing
an
overview
different
sensing
applications
while
highlighting
major
progress
and
seminal
contributions
to
the
field
portable
biosensor
devices
point-of-care
diagnostics.
Specifically,
functional
nucleic
acids
is
introduced,
specific
focus
on
DNAzymes.
The
incorporation
DNAzymes
into
bioassays
then
described,
followed
by
detailed
recent
advances
in
development
vivo
platforms
sensors
incorporating
for
molecular
recognition.
Finally,
critical
perspective
field,
summary
where
DNAzyme-based
may
make
biggest
impact
are
provided.
Chemical Reviews,
Journal Year:
2022,
Volume and Issue:
122(18), P. 14881 - 14910
Published: Sept. 6, 2022
Lateral
flow
assays
(LFAs)
are
currently
the
most
used
point-of-care
sensors
for
both
diagnostic
(e.g.,
pregnancy
test,
COVID-19
monitoring)
and
environmental
pesticides
bacterial
applications.
Although
core
of
LFA
technology
was
developed
several
decades
ago,
in
recent
years
integration
novel
nanomaterials
as
signal
transducers
or
receptor
immobilization
platforms
has
brought
improved
analytical
capabilities.
In
this
Review,
we
present
how
nanomaterial-based
LFAs
can
address
inherent
challenges
(PoC)
diagnostics
such
sensitivity
enhancement,
lowering
detection
limits,
multiplexing,
quantification
analytes
complex
samples.
Specifically,
highlight
strategies
that
synergistically
solve
limitations
current
have
proven
commercial
feasibility.
Finally,
discuss
barriers
toward
commercialization
next
generation
LFAs.
ACS Nano,
Journal Year:
2022,
Volume and Issue:
16(2), P. 3351 - 3361
Published: Feb. 9, 2022
Colorimetric
lateral
flow
immunoassay
(LFIA)
with
gold
nanoparticles
(AuNPs)
as
signal
reporters
has
been
widely
used
in
point-of-care
testing.
Nonetheless,
the
potential
of
traditional
AuNP-based
LFIA
for
early
diagnosis
disease
is
often
compromised
by
limited
sensitivity
due
to
insufficient
colorimetric
brightness
AuNPs.
Herein,
we
develop
a
"three-in-one"
multifunctional
catalytic
nanohybrid
(Fe3O4@MOF@Pt)
composed
Fe3O4
nanoparticles,
MIL-100(Fe),
and
platinum
(Pt)
nanoparticles.
Fe3O4@MOF@Pt
displays
enhanced
brightness,
fast
magnetic
response,
ultrahigh
peroxidase-mimicking
activity,
which
are
beneficial
enhancement
coupling
separation
amplification.
When
integrated
dual-antibody
sandwich
platform,
developed
can
achieve
an
ultrasensitive
immunochromatographic
assay
procalcitonin
0.5
pg
mL–1,
approximately
2280-fold
higher
than
that
conventional
superior
previously
published
immunoassays.
Therefore,
this
work
suggests
proposed
act
promising
enable
diagnostics.
Theranostics,
Journal Year:
2021,
Volume and Issue:
12(2), P. 574 - 602
Published: Dec. 15, 2021
Lateral
flow
immunoassay
(LFIA)
with
gold
nanoparticles
(AuNPs)
as
signal
reporters
is
a
popular
point-of-care
diagnostic
technique.
However,
given
the
weak
absorbance
of
traditional
20-40
nm
spherical
AuNPs,
their
sensitivity
low,
which
greatly
limits
wide
application
AuNP-based
LFIA.
With
rapid
advances
in
materials
science
and
nanotechnology,
synthesis
noble
metal
(NMNPs)
has
enhanced
physicochemical
properties
such
optical,
plasmonic,
catalytic,
multifunctional
activity
by
simply
engineering
physical
parameters,
including
size,
shape,
composition,
external
structure.
Using
these
engineered
NMNPs
an
alternative
to
LFIA
been
significantly
improved,
thereby
expanding
working
range
scenarios
LFIA,
particularly
trace
analysis.
Therefore,
this
review,
we
will
focus
on
design
demonstration
improving
We
highlight
strategies
available
for
tailoring
NMNP
designs,
effect
performance,
principle
each
enhancing
Finally,
current
challenges
future
improvements
field
are
briefly
discussed.
Biosensors,
Journal Year:
2021,
Volume and Issue:
11(9), P. 295 - 295
Published: Aug. 25, 2021
The
development
of
reliable
and
robust
diagnostic
tests
is
one
the
most
efficient
methods
to
limit
spread
coronavirus
disease
2019
(COVID-19),
which
caused
by
severe
acute
respiratory
syndrome
coronavirus-2
(SARS-CoV-2).
However,
laboratory
diagnostics
for
COVID-19,
such
as
enzyme-linked
immunosorbent
assay
(ELISA)
reverse
transcriptase-polymerase
chain
reaction
(RT-PCR),
are
expensive,
time-consuming,
require
highly
trained
professional
operators.
On
other
hand,
lateral
flow
immunoassay
(LFIA)
a
simpler,
cheaper
device
that
can
be
operated
unskilled
personnel
easily.
Unfortunately,
current
technique
has
some
limitations,
mainly
inaccuracy
in
detection.
This
review
article
aims
highlight
recent
advances
novel
technologies
detecting
SARS-CoV-2
well
innovative
approaches
achieve
sensitive
specific
point-of-care
testing.
Lastly,
we
discuss
future
perspectives
on
how
smartphones
Artificial
Intelligence
(AI)
integrated
revolutionize
detection
control
surveillance.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(17)
Published: Jan. 30, 2023
Abstract
Polymerase
chain
reaction
(PCR)‐based
nucleic
acid
testing
has
played
a
critical
role
in
disease
diagnostics,
pathogen
surveillance,
and
many
more.
However,
this
method
requires
long
turnaround
time,
expensive
equipment,
trained
personnel,
limiting
its
widespread
availability
diagnostic
capacity.
On
the
other
hand,
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
technology
recently
demonstrated
capability
for
detection
with
high
sensitivity
specificity.
CRISPR‐mediated
biosensing
holds
great
promise
revolutionizing
procedures
developing
point‐of‐care
diagnostics.
This
review
focuses
on
recent
developments
both
fundamental
CRISPR
biochemistry
CRISPR‐based
techniques.
Four
ongoing
research
hotspots
molecular
diagnostics‐target
preamplification‐free
detection,
microRNA
(miRNA)
testing,
non‐nucleic‐acid
SARS‐CoV‐2
detection‐are
also
covered.
