Fe-Single-Atom Nanozyme Catalysts for Sensitive and Selective Detection of Nitrite via Colorimetry and Test Strips

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 6(7), P. 5879 - 5888

Published: March 21, 2023

Here, a sensitive and selective strategy for the detection of nitrite via colorimetry test strips was developed based on Fe-single-atom catalysts (Fe SACs) through oxidation–reduction diazotization reactions. Fe SACs possess excellent oxidase-like (OXD) catalytic activity, which can catalyze oxidation colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue TMBox with appearance an obvious absorbance peak at 652 nm in presence oxygen. With addition (NO2–), reactions between TMB/TMBox induce color solution to change from green finally yellow, increase 445 nm. Based this strategy, dual-signal-ratio colorimetric method proposed. Within concentration range 1–120 μM, ratio A652/A445 has favorable linear relationship logarithm NO2–, limit 0.238 μM. By combining smartphones method, more intuitive, visual, convenient strip platform developed, be utilized within 2–200 The analysis nanozyme catalysis integrated specific redox/diazotization reaction not only provides dual-signal sensing good sensitivity but holds advantage selectivity utilization chemical reaction, broad application prospects food safety supervision screening.

Language: Английский

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Single-Atom Nanozymes: From Precisely Engineering to Extensive Applications

Accounts of Materials Research, Journal Year: 2024, Volume and Issue: 5(3), P. 347 - 357

Published: Feb. 3, 2024

ConspectusNanozymes are nanomaterials with intrinsic enzyme-like properties that can overcome the current limitations of natural enzymes, such as high preparation cost, instability, restricted application scenarios, etc. Since Fe3O4 nanoparticles (NPs) were shown to possess peroxidase (POD)-like activity in 2007, thousands reported mimic catalytic various types enzymes including catalase (CAT), haloperoxidase, superoxide dismutase (SOD), glucose oxidase, glutathione peroxidase, hydrolase, nuclease, nitroreductase, and others. Particularly, some nanozymes showed multienzyme-like activities regarding changes scenarios temperature, pH, Benefiting from their distinct physical-chemical characteristics properties, have been widely applied biomedical related fields vitro detections vivo therapeutic treatments. However, currently ambiguous structure–function correlations relatively inferior compared promote extensive efforts for modifications on development novel alternative nanozymes. The single-atom (SAzymes) present a unique way highly evolved enzyme active centers, because atomically dispersed sites, which leads atom utilization efficiency and, thus, potentially extraordinary activity. Also, abilities modify centers and/or tune interactions between metal supporting ligands provide precise engineer SAzymes at atomic levels. Given well-defined geometric electronic structures, thus serve exceptional templates deciphering relationships, is beneficial further improving performances.In this Account, we will review our recent other notable works developments effective mimics applications areas. We begin brief introduction why emergence SAzymes, artificial enzyme, tackles challenges facing. Next, focus systematic design, synthesis optimization especially impacts engineering environment an enzymologist perspective. For example, alternations first-shell ligand N P/S, SAzymes' CAT-like increased more than 4-fold. coordination numbers (x) Co–Nx(C) SAzyme significantly altered its oxidase (OXD)-like kinetics Then, discuss ways standardization specific kinetics. also wide ranges colorimetric biologicals, antibiosis treatments, cancer therapies. Finally, address future perspectives

Language: Английский

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Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(12), P. 3470 - 3488

Published: Aug. 15, 2024

Language: Английский

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Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy

Advanced Science, Journal Year: 2024, Volume and Issue: 11(16)

Published: Feb. 21, 2024

Abstract Cancer cells typically display redox imbalance compared with normal due to increased metabolic rate, accumulated mitochondrial dysfunction, elevated cell signaling, and accelerated peroxisomal activities. This may regulate gene expression, alter protein stability, modulate existing cellular programs, resulting in inefficient treatment modalities. Therapeutic strategies targeting intra‐ or extracellular states of cancer at varying state progression trigger programmed death if exceeded a certain threshold, enabling therapeutic selectivity overcoming resistance radiotherapy chemotherapy. Nanotechnology provides new opportunities for modulating their excellent designability high reactivity. Various nanomaterials are widely researched enhance highly reactive substances (free radicals) production, disrupt the endogenous antioxidant defense systems, both. Here, physiological features described challenges illustrated. Then, that classified elaborated upon based on ability target regulations. Finally, future perspectives this field proposed. It is hoped review guidance design nanomaterials‐based approaches involving therapy, especially cancers resistant chemotherapy, etc.

Language: Английский

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Brain‐Targeted HFn‐Cu‐REGO Nanoplatform for Site‐Specific Delivery and Manipulation of Autophagy and Cuproptosis in Glioblastoma

Small, Journal Year: 2022, Volume and Issue: 19(2)

Published: Nov. 18, 2022

Durable glioblastoma multiforme (GBM) management requires long-term chemotherapy after surgery to eliminate remaining cancerous tissues. Among chemotherapeutics, temozolomide is considered as the first-line drug for GBM therapy, but treatment outcome not satisfactory. Notably, regorafenib, an oral multi-kinase inhibitor, has been reported exert a markedly superior effect on suppression compared with temozolomide. However, poor site-specific delivery and bioavailability significantly restrict efficient permeability of regorafenib brain lesions compromise its efficacy. Therefore, human H-ferritin (HFn), Cu2+ are rationally designed brain-targeted nanoplatform (HFn-Cu-REGO NPs), fulfilling task manipulating autophagy cuproptosis against GBM. Herein, HFn affords preferential accumulation capacity due transferrin receptor 1 (TfR1)-mediated active targeting pH-responsive behavior. Moreover, can inhibit autophagosome-lysosome fusion, resulting in lethal arrest cells. Furthermore, only facilitates encapsulation through coordination interaction also disturbs copper homeostasis triggering cuproptosis, synergistical regorafenib-mediated this work may broaden clinical application scope via modulating cuproptosis.

Language: Английский

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Foldable paper microfluidic device based on single iron site-containing hydrogel nanozyme for efficient glucose biosensing

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 454, P. 140541 - 140541

Published: Nov. 23, 2022

Language: Английский

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Reactive oxygen species-upregulating nanomedicines towards enhanced cancer therapy

Biomaterials Science, Journal Year: 2022, Volume and Issue: 11(4), P. 1182 - 1214

Published: Dec. 20, 2022

A review of the recent progress ROS-upgrading nanosystems in oncotherapy is presented.

Language: Английский

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Copper-olsalazine metal-organic frameworks as a nanocatalyst and epigenetic modulator for efficient inhibition of colorectal cancer growth and metastasis

Acta Biomaterialia, Journal Year: 2022, Volume and Issue: 152, P. 495 - 506

Published: Sept. 8, 2022

Language: Английский

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The applications of nanozymes in cancer therapy: based on regulating pyroptosis, ferroptosis and autophagy of tumor cells

Nanoscale, Journal Year: 2023, Volume and Issue: 15(29), P. 12137 - 12156

Published: Jan. 1, 2023

Nanozymes are nanomaterials with catalytic properties similar to those of natural enzymes, and they have recently been collectively identified as a class innovative artificial enzymes.

Language: Английский

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A Multifunctional Covalent Organic Framework Nanozyme for Promoting Ferroptotic Radiotherapy against Esophageal Cancer

ACS Nano, Journal Year: 2023, Volume and Issue: 17(20), P. 20445 - 20461

Published: Oct. 6, 2023

Radiotherapy is inevitably accompanied by some degree of radiation resistance, which leads to local recurrence and even therapeutic failure. To overcome this limitation, herein, we report the room-temperature synthesis an iodine- ferrocene-loaded covalent organic framework (COF) nanozyme, termed TADI-COF-Fc, for enhancement radiotherapeutic efficacy in treatment radioresistant esophageal cancer. The iodine atoms on COF not only exerted a direct effect radiotherapy, increasing its X-ray absorption, but also promoted radiolysis water, increased production reactive oxygen species (ROS). In addition, ferrocene surface decoration disrupted redox homeostasis levels hydroxyl lipid peroxide radicals depleting intracellular antioxidants. Both vitro vivo experiments substantiated excellent response TADI-COF-Fc. This study demonstrates potential COF-based multinanozymes as radiosensitizers suggests possible integration strategy combination oncotherapy.

Language: Английский

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