Single-shot wavelength-multiplexed phase microscopy under Gabor regime in a regular microscope embodiment

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: March 14, 2023

Phase imaging microscopy under Gabor regime has been recently reported as an extremely simple, low cost and compact way to update a standard bright-field microscope with coherent sensing capabilities. By inserting illumination in the embodiment producing small defocus distance of sample at input plane, digital sensor records in-line hologram target sample, which is then numerically post-processed finally achieve sample's quantitative phase information. However, retrieved distribution affected by two well-known drawbacks when dealing Gabor's regime, that is, noise twin image disturbances. Here, we present single-shot technique based on wavelength multiplexing for mitigating these effects. A multi-illumination laser source (including 3 diode lasers) illuminates color (conventional RGB camera) used record wavelength-multiplexed single exposure. The completed presenting novel algorithm modified Gerchberg-Saxton kernel retrieve enhanced terms removal minimization. Experimental validations are performed regular Olympus BX-60 upright using 20X 0.46NA objective lens considering static (resolution test targets) dynamic (living spermatozoa) samples.

Language: Английский

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Digital in-line holographic microscopy for label-free identification and tracking of biological cells

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: June 13, 2024

Abstract Digital in-line holographic microscopy (DIHM) is a non-invasive, real-time, label-free technique that captures three-dimensional (3D) positional, orientational, and morphological information from digital images of living biological cells. Unlike conventional microscopies, the DIHM enables precise measurements dynamic behaviors exhibited by cells within 3D volume. This review outlines fundamental principles comprehensive image processing procedures employed in DIHM-based cell tracking methods. In addition, recent applications for identification various motile cells, including human blood spermatozoa, diseased unicellular microorganisms, are thoroughly examined. Leveraging artificial intelligence has significantly enhanced both speed accuracy identification. The quantitative data on morphology dynamics captured can effectively elucidate underlying mechanisms governing microbial contribute to accumulation diagnostic databases development clinical treatments.

Language: Английский

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Perspective on quantitative phase imaging to improve precision cancer medicine

Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(S2)

Published: March 26, 2024

SignificanceQuantitative phase imaging (QPI) offers a label-free approach to non-invasively characterize cellular processes by exploiting their refractive index based intrinsic contrast. QPI captures this contrast translating associated shifts into intensity-based quantifiable data with nanoscale sensitivity. It holds significant potential for advancing precision cancer medicine providing quantitative characterization of the biophysical properties cells and tissue in natural states.AimThis perspective aims discuss increase our understanding development its response therapeutics. also explores new developments methods towards personalized therapy early detection.ApproachWe begin detailing technical advancements QPI, examining implementations across transmission reflection geometries retrieval methods, both interferometric non-interferometric. The focus then QPI's applications research, including dynamic cell mass drug assessment, risk stratification, in-vivo imaging.ResultsQPI has emerged as crucial tool medicine, offering insights tumor biology treatment efficacy. Its sensitivity detecting changes promise enhancing diagnostics, prognostication. future is envisioned integration artificial intelligence, morpho-dynamics, spatial biology, broadening impact research.ConclusionsQPI presents redefining diagnosis, monitoring, treatment. Future directions include harnessing high-throughput imaging, 3D realistic models, combining intelligence multi-omics extend capabilities. As result, stands at forefront research clinical application care.

Language: Английский

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Microsphere-assisted quantitative phase microscopy: a review

Deleted Journal, Journal Year: 2024, Volume and Issue: 5(1), P. 1 - 1

Published: Jan. 1, 2024

Light microscopes are the most widely used devices in life and material sciences that allow study of interaction light with matter at a resolution better than naked eye. Conventional translate spatial differences intensity reflected or transmitted from an object to pixel brightness digital image. However, phase microscope converts through brightness. Interference microscopy, phase-based approach, has found application various disciplines. While interferometry brought nanometric axial resolution, lateral quantitative microscopy (QPM) still remained limited by diffraction, similar other traditional systems. Enhancing been subject intense investigation since invention 17th century. During past decade, microsphere-assisted (MAM) emerged as simple effective approach enhance microscopy. MAM can be integrated QPM for 3D label-free imaging enhanced resolution. Here, we review integration microspheres coherence scanning interference holographic microscopies, discussing associated open questions, challenges, opportunities.

Language: Английский

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Quantitative phase analysis and hyperspectral imaging for the automatic identification of veins and blood perfusion maps

Photodiagnosis and Photodynamic Therapy, Journal Year: 2023, Volume and Issue: 42, P. 103307 - 103307

Published: Jan. 25, 2023

Language: Английский

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Ultimate Quantum Sensitivity in the Estimation of the Delay between two Interfering Photons through Frequency-Resolving Sampling

Physical Review Applied, Journal Year: 2023, Volume and Issue: 19(4)

Published: April 24, 2023

We demonstrate the ultimate sensitivity allowed by quantum physics in estimation of time delay between two photons measuring their interference at a beam splitter through frequency-resolving sampling measurements. This can be increased quadratically decreasing photonic temporal bandwidth even values smaller than when standard two-photon interferometers become inoperable and without adapting path reference photon, nor need time-resolving detectors with an unfeasible high resolution. Applications range from more feasible imaging nanostructures, including biological samples, nanomaterial surfaces to enhanced based on frequency-resolved boson optical networks.

Language: Английский

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QPI assay of fibroblasts resilience to adverse effects of nanoGO clusters by multimodal and multiscale microscopy

Journal of Physics Photonics, Journal Year: 2024, Volume and Issue: 6(1), P. 015004 - 015004

Published: Jan. 1, 2024

Abstract Graphene is considered a possible drug deliver in nanomedicine for its mechanical, physical and chemical characteristics. Thus, studying graphene biocompatibility pivotal to contribute the modern nano-therapy science. The coexistence between cells should be analysed using non-invasive technologies thus quantitative phase imaging (QPI) modalities are suitable investigate morphometric evolution of under nanomaterial exposure. Here, we show how multimodal QPI approach can furnish noninvasive analysis probing dose-dependent effect nanoGO clusters on adherent NIH 3T3 fibroblast cells. We rely both digital holography Fourier ptychography (FP) transmission microscopy mode. former allows accurate time-lapse experiments at single cell level. latter provides wide field view characterization network level, assuring significant statistical measurement by exploiting intrinsic large space-bandwidth product FP. combination these two techniques one extract information about resilience adverse effects surrounding buffer, namely through quantitative, multi-scale, time-resolved characterization.

Language: Английский

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Single-Shot Intensity- and Phase-Sensitive Compressive Sensing-Based Coherent Modulation Ultrafast Imaging

Physical Review Letters, Journal Year: 2024, Volume and Issue: 132(17)

Published: April 24, 2024

Ultrafast imaging can capture the dynamic scenes with a nanosecond and even femtosecond temporal resolution. Complementarily, phase provide morphology, refractive index, or thickness information that intensity cannot represent. Therefore, it is important to realize simultaneous ultrafast for achieving as much possible in detection of scenes. Here, we report single-shot intensity- phase-sensitive compressive sensing-based coherent modulation technique, shortened CS-CMUI, which integrates imaging, streak imaging. We theoretically demonstrate through numerical simulations CS-CMUI obtain both ultrahigh fidelity. Furthermore, experimentally build system successfully measure evolution multimode $Q$-switched laser pulse dynamical behavior ablation on an indium tin oxide thin film. It anticipated enables profound comprehension phenomena promotes advancement various practical applications, will have substantial impact fundamental applied sciences.

Language: Английский

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Spin–orbit optical broadband achromatic spatial differentiation imaging

Optica, Journal Year: 2024, Volume and Issue: 11(7), P. 1008 - 1008

Published: June 24, 2024

Spatial optical analog differentiation allows ultrahigh-speed and low-power-consumption of image processing, as well label-free imaging transparent biological objects. Optical with broadband incoherent sources is appealing for its multi-channels multi-task information the high-quality imaging. Currently, still challenging. Here, a compact achromatic spatial differentiator demonstrated based on intrinsic spin–orbit coupling in natural thin crystal. By inserting uniaxial crystal just before camera conventional microscope, spin to orbit conversion will embed an vortex field make second-order topological field, thus isotropic differential be captured by camera. The wavelength-independent property effect us achieve computing With this method, both amplitude pure phase objects are detected high contrast. Transparent living cells tissues imaged their edge contours intracellular details protruded detection mode enhancement mode, respectively. These findings pave way light concurrently drive advancement high-performance cost-effective contrast

Language: Английский

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Multi-wavelength interference phase imaging for automatic breast cancer detection and delineation using diffuse reflection imaging

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 3, 2024

Abstract Millions of women globally are impacted by the major health problem breast cancer (BC). Early detection BC is critical for successful treatment and improved survival rates. In this study, we provide a progressive approach using multi-wavelength interference (MWI) phase imaging based on diffuse reflection hyperspectral (HS) imaging. The proposed findings measurement pattern between blue (446.6 nm) red (632 wavelengths. We consider implementing comprehensive image processing categorization method use Fast Fourier (FF) transform analysis pertaining to change in refractive index tumor normal tissue. observed that growth affects tissue organization dramatically, as seen persistently increased variance tumors compared areas. Both malignant had different depth data collected from it was analyzed. To enhance ex-vivo tissue, developed validated training classifier algorithm specifically designed categorizing HS cube data. Following application signal normalization with FF algorithm, our methodology achieved high level performance specificity ( Spec ) 94% sensitivity Sen 90.9% 632 nm acquired categorization, preliminary specimens under investigation. Notably, successfully leveraged unstained samples create 3D phase-resolved images effectively highlight distinctions reflectance features cancerous healthy Preliminary revealed might be able assist specialists safely excising areas assessing bed following resection automatically at depths. This investigation result an effective "in-vivo" disease description utilizing optical technology typical RGB camera wavelength-specific operation quantitative MWI methodology.

Language: Английский

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