The Emerging Role of Raman Spectroscopy as an Omics Approach for Metabolic Profiling and Biomarker Detection toward Precision Medicine

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(13), P. 8297 - 8346

Published: June 15, 2023

Omics technologies have rapidly evolved with the unprecedented potential to shape precision medicine. Novel omics approaches are imperative toallow rapid and accurate data collection integration clinical information enable a new era of healthcare. In this comprehensive review, we highlight utility Raman spectroscopy (RS) as an emerging technology for clinically relevant applications using significant samples models. We discuss use RS both label-free approach probing intrinsic metabolites biological materials, labeled where signal from reporters conjugated nanoparticles (NPs) serve indirect measure tracking protein biomarkers

Language: Английский

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Nanomaterial-based contrast agents

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: April 13, 2023

Language: Английский

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Artificial Intelligence for Surface‐Enhanced Raman Spectroscopy

Small Methods, Journal Year: 2023, Volume and Issue: 8(1)

Published: Oct. 27, 2023

Abstract Surface‐enhanced Raman spectroscopy (SERS), well acknowledged as a fingerprinting and sensitive analytical technique, has exerted high applicational value in broad range of fields including biomedicine, environmental protection, food safety among the others. In endless pursuit ever‐sensitive, robust, comprehensive sensing imaging, advancements keep emerging whole pipeline SERS, from design SERS substrates reporter molecules, synthetic route planning, instrument refinement, to data preprocessing analysis methods. Artificial intelligence (AI), which is created imitate eventually exceed human behaviors, exhibited its power learning high‐level representations recognizing complicated patterns with exceptional automaticity. Therefore, facing up intertwining influential factors explosive size, AI been increasingly leveraged all above‐mentioned aspects presenting elite efficiency accelerating systematic optimization deepening understanding about fundamental physics spectral data, far transcends labors conventional computations. this review, recent progresses are summarized through integration AI, new insights challenges perspectives provided aim better gear toward fast track.

Language: Английский

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Biomedical SERS – the current state and future trends

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(18), P. 8957 - 8979

Published: Jan. 1, 2024

Surface enhanced Raman spectroscopy (SERS) is meeting the requirements in biomedical science being a highly sensitive and specific analytical tool. By employing portable systems combination with customized sample pre-treatment, point-of-care-testing (POCT) becomes feasible. Powerful SERS-active sensing surfaces high stability modification layers if required are available for testing application complex biological matrices such as body fluids, cells or tissues. This review summarizes current state collection pretreatment SERS detection protocols, schemes,

Language: Английский

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Convergence of Surface-Enhanced Raman Scattering with Molecular Diagnostics: A Perspective on Future Directions

ACS Nano, Journal Year: 2024, Volume and Issue: 18(8), P. 5998 - 6007

Published: Feb. 12, 2024

Molecular diagnostics (MD) is widely employed in multiple scientific disciplines, such as oncology, pathogen detection, forensic investigations, and the pharmaceutical industry. Techniques polymerase chain reaction (PCR) revolutionized rapid accurate identification of nucleic acids (DNA, RNA). More recently, CRISPR its CRISPR-associated protein (Cas) have been a ground-breaking discovery that latest revolution molecular biology, including MD. Surface-enhanced Raman scattering (SERS) very attractive alternative to fluorescence currently most used optical readout In this Perspective, milestones development MD, SERS-PCR, next-generation approaches Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK) DNA Endonuclease-Targeted Trans (DETECTR), are briefly summarized. Our perspective on future convergence SERS with MD focused SERS-based CRISPR/Cas (SERS-CRISPR) since we anticipate many promising applications rapidly emerging field. We predict major developments will exploit advantages real-time monitoring superior brightness, photostability, spectral multiplexing potential nanotags an automated workflow for assays under isothermal, amplification-free conditions.

Language: Английский

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Plasmonic nanoparticle sensors: current progress, challenges, and future prospects

Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 9(12), P. 2085 - 2166

Published: Jan. 1, 2024

This comprehensive review summarizes the past, present, and future of plasmonic NP-based sensors in terms different sensing platforms, chemical biological analytes, expected technologies.

Language: Английский

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Advancing SERS as a quantitative technique: challenges, considerations, and correlative approaches to aid validation

Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)

Published: Aug. 17, 2024

Surface-enhanced Raman scattering (SERS) remains a significant area of research since it's discovery 50 years ago. The surface-based technique has been used in wide variety fields, most prominently chemical detection, cellular imaging and medical diagnostics, offering high sensitivity specificity when probing quantifying chosen analyte or monitoring nanoparticle uptake accumulation. However, despite its promise, SERS is mostly confined to academic laboratories not recognised as gold standard analytical technique. This due the variations that are observed measurements, mainly caused by poorly characterised substrates, lack universal calibration methods uncorrelated results. To convince wider scientific community should be routinely technique, field now focusing on will increase reproducibility signals how validate results with more well-established techniques. review explores difficulties experienced users, adopted reduce variation suggestions best practices strategies if one achieve absolute quantification.

Language: Английский

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Molecular imaging: design mechanism and bioapplications

Science China Chemistry, Journal Year: 2023, Volume and Issue: 66(5), P. 1336 - 1383

Published: March 3, 2023

Language: Английский

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Current strategies of plasmonic nanoparticles assisted surface-enhanced Raman scattering toward biosensor studies

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 228, P. 115231 - 115231

Published: March 15, 2023

Language: Английский

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Highly Excretable Gold Supraclusters for Translatable In Vivo Raman Imaging of Tumors

ACS Nano, Journal Year: 2023, Volume and Issue: 17(3), P. 2554 - 2567

Published: Jan. 23, 2023

Raman spectroscopy provides excellent specificity for in vivo preclinical imaging through a readout of fingerprint-like spectra. To achieve sufficient sensitivity imaging, metallic gold nanoparticles larger than 10 nm were employed to amplify signals via surface-enhanced scattering (SERS). However, the inability excrete such large has restricted translation imaging. Here we present Raman-active supraclusters that are biodegradable and excretable as nanoclusters. Although small size nanocluster building blocks compromises electromagnetic field enhancement effect, exhibit bright prominent comparable nanoparticle-based SERS nanotags due high loading NIR-resonant dyes much suppressed fluorescence background by supraclusters. The was pH-responsive, successfully performed acidic tumors mice. Furthermore, contrast remain liver spleen over 4 months, dissociated into nanoclusters, 73% administered dose mice excreted during same period. highly demonstrated here offer great potential clinical applications

Language: Английский

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