Hydrogel-Coated SERS Microneedles for Drug Monitoring in Dermal Interstitial Fluid

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(5), P. 2567 - 2574

Published: May 2, 2024

In vivo drug monitoring is crucial for evaluating the effectiveness and safety of treatment. Blood sampling analysis current gold standard but needs professional skills cannot meet requirements point-of-care testing. Dermal interstitial fluid (ISF) showed great potential to replace blood in monitoring; however, detection was challenging, distribution behavior ISF still unclear until now. this study, we proposed surface-enhanced Raman spectroscopy (SERS) microneedles (MNs) painless real-time drugs after intravenous injection. Using methylene blue (MB) mitoxantrone (MTO) as model drugs, innovative core–satellite structured Au@Ag SERS substrate, hydrogel coating over MNs, rendered sensitive quantitative mice within 10 min. Based on technique, pharmacokinetics two investigated compared with those blood, where were analyzed via liquid chromatography–mass spectrometry. It found that MB concentration comparable, whereas MTO 2–3 orders magnitude lower than blood. This work an efficient tool monitoring. More importantly, it experimentally proved penetration ratio drug-dependent, providing insightful information into a alternative detection.

Language: Английский

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Nanozyme-based wearable biosensors for application in healthcare

iScience, Journal Year: 2025, Volume and Issue: 28(2), P. 111763 - 111763

Published: Jan. 7, 2025

Recent years have witnessed tremendous advances in wearable sensors, which play an essential role personalized healthcare for their ability real-time sensing and detection of human health information. Nanozymes, capable mimicking the functions natural enzymes addressing limitations, possess unique advantages such as structural stability, low cost, ease mass production, making them particularly beneficial constructing recognition units biosensors. In this review, we aim to delineate latest advancements nanozymes development biosensors, focusing on key developments nanozyme immobilization strategies, technologies, biomedical applications. The review also highlights current challenges future perspectives. Ultimately, it aims provide insights research endeavors rapidly evolving area.

Language: Английский

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“Three-in-One” Plasmonic Au@PtOs Nanocluster Driven Lateral Flow Assay for Multimodal Cancer Exosome Biosensing

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(26), P. 10686 - 10695

Published: June 17, 2024

Exploiting the multiple properties of nanozymes for multimode lateral flow assay (LFA) is urgently required to improve accuracy and versatility. Herein, we developed a novel plasmonic Au nanostar@PtOs nanocluster (Au@PtOs) as signal tag LFA detection. Based on PtOs bimetallic doping strategy, Au@PtOs can indicate both excellent SERS enhancement nanozyme catalytic activity. Meanwhile, displays better photothermal effect than that nanostars. Therefore, colorimetric/SERS/temperature three-mode signals be read out based nanocomposite. The was combined with applied breast cancer exosome detection limit mode 2.6 × 103/4.1 101/4.6 102 exosomes/μL, respectively, which much superior common nanoparticles (∼105 exosomes/μL). Moreover, fingerprint molecular recognition ability mode, phenotypes derived from different cell lines discriminated easily. convenient visual colorimetric sensitive SERS/temperature quantitative modes, driven satisfy requirements accurate flexible multimodal sensing in application scenarios.

Language: Английский

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A self-powered controllable microneedle drug delivery system for rapid blood pressure reduction

Nano Energy, Journal Year: 2024, Volume and Issue: 123, P. 109344 - 109344

Published: Jan. 29, 2024

Language: Английский

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On‐Site Melanoma Diagnosis Utilizing a Swellable Microneedle‐Assisted Skin Interstitial Fluid Sampling and a Microfluidic Particle Dam for Visual Quantification of S100A1

Advanced Science, Journal Year: 2024, Volume and Issue: 11(16)

Published: Feb. 28, 2024

Abstract Malignant melanoma (MM) is the most aggressive form of skin cancer. The delay in treatment will induce metastasis, resulting a poor prognosis and even death. Here, two‐step strategy for on‐site diagnosis MM developed based on extraction direct visual quantification S100A1, biomarker melanoma. First, swellable microneedle utilized to extract S100A1 interstitial fluid (ISF) with minimal invasion. After elution, antibody‐conjugated magnetic microparticles (MMPs) polystyrene (PMPs) are introduced. A high expression level gives rise robust binding between MMPs PMPs reduces number free PMPs. By loading reacted solution into device microfluidic particle dam, quantity after separation displayed their accumulation length inversely proportional levels. limit detection 18.7 ng mL −1 achieved. animal experiment indicates that ISF‐based using proposed exhibits significantly higher sensitivity compared conventional serum‐based detection. In addition, result highly comparable gold standard enzyme‐linked immunosorbent assay Lin's concordance correlation coefficient, suggesting practicality routine monitoring

Language: Английский

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A “dual-key-and-lock” molecular probe for accurate diagnosis of melanoma

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 409, P. 135572 - 135572

Published: March 2, 2024

Language: Английский

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Theory of nanostructured sensors integrated in/on microneedles for diagnostics and therapy

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 255, P. 116238 - 116238

Published: March 28, 2024

Language: Английский

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Light‐Promoted Extraction of Precious Metals Using a Porphyrin‐integrated One‐dimensional Covalent Organic Framework

Angewandte Chemie, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Abstract Precious metals are valuable materials for the chemical industry, but they scarce and pose a risk of supply disruption. Recycling precious from waste is promising strategy, here we tactfully utilize light irradiation as an environmental‐friendly energy‐saving adjunctive strategy to promote reduction metal ions, thereby improving adsorption capacity kinetics. A newly light‐sensitive covalent organic framework (PP‐COF) was synthesized illustrate effectiveness feasibility this auxiliary strategy. The equilibrium capacities PP‐COF with towards gold, platinum, silver ions 4729, 573, 519 mg g −1 , which 3.3, 1.9, 1.2 times under dark condition. Significantly, filtration column can recover more than 99.8 % gold in simulated e‐waste leachates irradiation, 1 gram up 0.15 tonne leachates. Interestingly, captured by mainly exist micron‐sized particles, be easily separated extraction. We believe work contribute recovery circular economy recycling resources.

Language: Английский

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Multifunctional Polymeric Nanoneedles with “Full‐Spectrum Intrinsic Internal Standard” for Precise SERS Biosensing

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

Abstract Polymeric micro and nanoneedles have an exceptionally flexible bio‐SERS structure. However, during detection, Raman signals from the polymer often overlap with those target molecules, which is unavoidable severely compromises accuracy of results. To address this issue, study designed fabricated a silver (poly(vinyl pyrrolidone)‐coated, PVP)/poly(vinylidene fluoride) (PVDF)/gold structure (Ag/PVDF/Au NNs) proposed full‐spectrum intrinsic internal standard (FS‐IS) analytical method. In method, entire spectra PVP PVDF within fingerprint region are used as standard, machine learning algorithms employed to quantitatively analyze relevant SERS detection statistical perspective. Both experimental theoretical results demonstrate that method more accurate than traditional analysis methods based on embedded or standards. Using FS‐IS Ag/PVDF/Au NNs successfully applied various biosensing applications, including cancer cell imaging in situ monitoring antibiotics urine surgical patients. This effectively addresses issue low nanoneedle structures provides valuable insights for development other high‐precision technologies substrates.

Language: Английский

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Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis

Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 15(2), P. 101085 - 101085

Published: Aug. 27, 2024

Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis therapy bacterial infections. Herein, we developed a close-packed ordered Au@AgPt array coupled with cascade triggering strategy surface-enhanced Raman scattering (SERS) colorimetric identification Staphylococcus aureus biomarker micrococcal nuclease (MNase) in serum samples. The trimetallic nanozymes can catalyze oxidation 3,3',5,5'-tetramethylbenzidine (TMB) molecules to SERS-enhanced oxidized TMB (oxTMB), accompanied by color change from colorless blue. In presence S. aureus, secreted MNase preferentially cut nucleobase AT-rich regions DNA sequences on magnetic beads (MBs) release alkaline phosphatase (ALP), which subsequently mediated oxTMB reduction inducing colorimetric/SERS signal fade away. Using this "on-to-off" strategy, target be recorded wide linear range limit 38 CFU/mL mode 6 SERS mode. Meanwhile, MNase-mediated characterized high specificity sensitivity successfully discriminated between patients sepsis (n = 7) healthy participants 3), as well monitored prognostic progression disease 2). Overall, benefiting highly active dense "hot spot" substrate, response dual-signal output, methodology will offer promising avenue infection.

Language: Английский

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