Nanomedicine, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 3
Published: Dec. 10, 2024
Language: Английский
Small, Journal Year: 2025, Volume and Issue: 21(9)
Published: Jan. 19, 2025
Abstract Rare genetic diseases (RGDs) affect a small percentage of the global population but collectively have substantial impact due to their diverse manifestations. Although precise reasons behind these remain unclear, roughly 80% cases are genetically linked. Recent efforts focus on understanding pathology and developing new diagnostic therapeutic approaches for RGDs. However, there persists gap between fundamental research clinical approaches, where advancements in nanotechnology offer promising improvements. In this context, nanosized light‐emitting quantum dots (QDs), ranging from 2–10 nm, materials applications. Their size‐tunable light emission, high yield, photostability allow tracking cargo. Additionally, QDs can be functionalized with agents, antibodies, or peptides target specific cellular pathways, enhancing treatment efficacy while minimizing side effects. By combining capabilities single platform, thus versatile powerful approach tackle rare disorders. Despite several reviews various applications QDs, utilization domain RGDs is not well documented. This review highlight QDs’ potential diagnosing treating certain addresses challenges limiting application.
Language: Английский
Citations
0
Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111680 - 111680
Published: Feb. 1, 2025
Language: Английский
Citations
0
Nano Letters, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
Clustered regularly interspaced short palindromic repeat (CRISPR) gene editing has poor efficacy and off-target side effect concerns. We herein report a semiconducting polymer (SP)-based nanoCRISPR system to improve CRISPR delivery allow for near-infrared (NIR) photoactivatable cancer therapy. An amphiphilic SP acts as photothermal converter, its backbone is grafted with single-stranded deoxyribonucleic acid (DNA), which enables hybridization single guide ribonucleic (sgRNA) via complementary base pairing form sgRNA/SP-DNA. This sgRNA/SP-DNA nanosystem (nanoCRISPR) can effectively deliver sgRNA into cells generate heat under NIR laser irradiation the effect. The localized triggers dissociation of DNA control release sgRNA, thereby achieving precise regulation activity. technology able precisely regulate expression green fluorescent protein (GFP) polo-like kinase 1 (PLK1) precision
Language: Английский
Citations
0
Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: unknown, P. 103497 - 103497
Published: March 1, 2025
Language: Английский
Citations
0
Nanomaterials, Journal Year: 2025, Volume and Issue: 15(7), P. 540 - 540
Published: April 2, 2025
Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR-Cas9), an emerging gene-editing technology, has recently gained rapidly increasing attention. However, the lack of efficient delivery vectors to deliver CRISPR-Cas9 specific cells or tissues hindered translation this biotechnology into clinical applications. Chemically synthesized nanoparticles (NPs), as attractive non-viral platforms for CRISPR-Cas9, have been extensively investigated because their unique characteristics, such controllable size, high stability, multi-functionality, bio-responsive behavior, biocompatibility, and versatility in chemistry. In review, key considerations precise design chemically synthesized-based include encapsulation, cellular uptake, targeting cells, endosomal escape, controlled release. We discuss cutting-edge strategies integrate chemical modifications that guide genome-editing machinery edits. also highlighted rationale intelligent nanoparticle design. particular, we summarized promising functional groups molecules can effectively optimize carrier function. addition, review focuses on advances widespread application NPs biomedical fields promote development safe, specific, delivering systems, providing references accelerating translational
Language: Английский
Citations
0
International Materials Reviews, Journal Year: 2025, Volume and Issue: unknown
Published: April 13, 2025
In recent years, zwitterionic materials have been widely used in biomedical and nanomedical due to their unique structure which the molecule is electrically neutral with equal positive negative charge. This kind of material could form a thick hydration layer by electrostatic effect, prevent contamination, show wide applications life science fields, such as resisting non-specific protein adsorption, biofilm, thrombosis formation, so on. Most importantly, diversity functional groups has made it more convenient for surface modification further utilization, creating multifunctional various applications. review summarized up-to-date materials, including conventional novel structural characteristics, well blood-contacting implantable devices, antibacterial coatings, wound dressings, drug-targeted carriers, smart wearable biosensors, especially clinical uses. New discoveries, existing challenges, commercial status are also outlined, future opportunities development perspectives new biomaterials demonstrated.
Language: Английский
Citations
0
Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 253, P. 114734 - 114734
Published: April 24, 2025
Language: Английский
Citations
0
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(35), P. 45871 - 45887
Published: Aug. 20, 2024
The long noncoding RNAs (lncRNA) are primarily associated with several essential gene regulations but also connected to cancer metabolism and progression. HOTAIR MALAT1 two such lncRNAs that detected in malignancies of various origins responsible for the poor prognosis patients. Due these factors, have emerged as prime targets development anticancer therapeutics. However, nonviral delivery lncRNA-targeted antisense oligonucleotides (ASOs) still remains a critical challenge while maintaining their structural functional integrity. Herein, we designed synthesized new series ionizable lipids variations head groups prepare lipid nanoparticle (LNP) formulation along cholesterol-based twin cationic amphiphilic zwitterionic lipid. context responsiveness formulations delivering ASOs has been thoroughly investigated by bioanalytical techniques, an optimum identified. LNPs utilized deliver targeting lncRNA human cell lines mouse models. This study thus standardizes advanced nanomaterial system validated considerable reduction target level under vitro significant tumor volume vivo settings.
Language: Английский
Citations
3
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 30, 2024
Epithelial cell adhesion molecule (EpCAM) gene encodes a type-I trans-membrane glycoprotein that is overexpressed in many cancerous epithelial cells and promotes tumor progression by regulating the expression of several oncogenes like c-myc other cyclins. Because this tumorigenic association, EpCAM has been potential target for anti-cancer therapy recent days. Herein, it attempted to knockout proto-oncogenic efficiently delivering an all-in-one Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) plasmid via lipid nanoparticle system made out synthetic stimuli-sensitive lipids. The possesses necessary information form guide RNA targeted gene. aptamer decorated selectively targets inhibits genetic expression. It explored pH-responsive property developed nanoparticles monitored their efficacy various cancer lines different origins with elevated levels. phenomenon further validated vivo non-immunocompromised mouse models. Overall, newly proven be efficacious delivery EpCAM-targeted CRISPR/Cas9 plasmid.
Language: Английский
Citations
2
Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14
Published: Sept. 20, 2024
Breast cancer (BC) is one of the frequent tumors that seriously endanger physical and mental well-being in women with strong heterogeneity, its pathogenesis involves multiple risk factors. Depending on type BC, hormonal therapy, targeted immunotherapy are current systemic treatment options along conventional chemotherapy. Despite significant progress understanding BC therapeutic options, there still a need to identify new targets develop more effective treatments. According recent sequencing profiling studies, non-coding (nc) RNAs genes deregulated human cancers via deletion, amplification, abnormal epigenetic, or transcriptional regulation, similarly, expression many ncRNAs altered breast cell lines tissues. The ability single regulate downstream gene related pathways provides theoretical basis for studying them drug development delivery. Therefore, it far-reaching explore role tumor their potential as targets. Here, our review outlines two major ncRNAs, long (lncRNAs) microRNAs (miRNAs) diagnostic prognostic biomarkers well strategies cancer.
Language: Английский
Citations
1