Nanoparticle contrast agents for X‐ray imaging applications DOI Creative Commons
Jessica C. Hsu, Lenitza M. Nieves, Oshra Betzer

et al.

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2020, Volume and Issue: 12(6)

Published: May 22, 2020

Abstract X‐ray imaging is the most widely used diagnostic method in modern medicine and several advanced forms of this technology have recently emerged. Iodinated molecules barium sulfate suspensions are clinically approved contrast agents used. However, these existing provide limited information, suboptimal for new techniques developing safety concerns. Thus, over past 15 years, there has been a rapid growth development nanoparticles as agents. Nanoparticles desirable features such high payloads, potential long circulation times, tunable physicochemical properties. also range biomedical applications disease treatment, targeted imaging, cell tracking. In review, we discuss principles behind generation introduce types modalities, well elements chemical compositions that suitable novel agent development. We focus on progress nanoparticle developed to be renally clearable, circulating, theranostic, targeted, or feature conjunction with newly multi‐energy computed tomography mammographic technologies. Finally, offer perspectives current limitations emerging research topics expectations future field. This article categorized under: Diagnostic Tools > vivo Nanodiagnostics Imaging Nanotechnology Approaches Biology Nanoscale Systems

Language: Английский

Exosomes‐Loaded Electroconductive Hydrogel Synergistically Promotes Tissue Repair after Spinal Cord Injury via Immunoregulation and Enhancement of Myelinated Axon Growth DOI
Lei Fan, Can Liu,

Xiuxing Chen

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(13)

Published: March 6, 2022

Electroconductive hydrogels are very attractive candidates for accelerated spinal cord injury (SCI) repair because they match the electrical and mechanical properties of neural tissue. However, electroconductive hydrogel implantation can potentially aggravate inflammation, hinder its efficacy. Bone marrow stem cell-derived exosomes (BMSC-exosomes) have shown immunomodulatory tissue regeneration effects, therefore, tissue-like loaded with BMSC-exosomes developed synergistic treatment SCI. These exosomes-loaded modulate microglial M2 polarization via NF-κB pathway, synergistically enhance neuronal oligodendrocyte differentiation cells (NSCs) while inhibiting astrocyte differentiation, also increase axon outgrowth PTEN/PI3K/AKT/mTOR pathway. Furthermore, combined significantly decrease number CD68-positive microglia, local NSCs recruitment, promote axonal regeneration, resulting in significant functional recovery at early stage an SCI mouse model. Hence, findings this study demonstrate that combination is a promising therapeutic strategy repair.

Language: Английский

Citations

253
Treatment of spinal cord injury with mesenchymal stem cells DOI Creative Commons

Ling Ling Liau,

Qi Hao Looi,

Wui Chuen Chia

et al.

Cell & Bioscience, Journal Year: 2020, Volume and Issue: 10(1)

Published: Sept. 22, 2020

Spinal cord injury (SCI) is the damage to spinal that can lead temporary or permanent loss of function due nerve. The SCI patients are often associated with poor quality life.This review discusses current status mesenchymal stem cell (MSC) therapy for SCI, criteria considering application MSC and novel biological therapies be applied together MSCs enhance its efficacy. Bone marrow-derived (BMSCs), umbilical cord-derived (UC-MSCs) adipose tissue-derived (ADSCs) have been trialed treatment SCI. Application may minimize secondary protect neural elements survived initial mechanical insult by suppressing inflammation. Additionally, shown differentiate into neuron-like cells stimulate proliferation rebuild damaged nerve tissue.These characteristics crucial restoration upon as has limited regenerative capacity it also something cannot achieved pharmacological physiotherapy interventions. New including secretome therapy, immunotherapy scaffolds combined therapeutic effects.

Language: Английский

Citations

164
Mesenchymal Stem Cell–Derived Exosomes: A Promising Biological Tool in Nanomedicine DOI Creative Commons

Wumei Wei,

Qiang Ao, Xiaohong Wang

et al.

Frontiers in Pharmacology, Journal Year: 2021, Volume and Issue: 11

Published: Jan. 25, 2021

As nano-scale biological vesicles, extracellular vesicles (EVs)/exosomes, in particular, exosomes derived from mesenchymal stem cells (MSC-exosomes), have been studied the diagnosis, prevention, and treatment of many diseases. In addition, through combination nanotechnology biotechnology, emerged as innovative tools for development nanomedicine. This review focuses on a profound summarization MSC-exosomes powerful tool bionanomedicine. It systemically summarizes role nanocarrier, drug loading tissue engineering, their potential contribution series diseases well advantages over synthetic nanoparticles disadvantages. The in-depth understanding functions mechanisms provides insights into basic research clinical transformation field

Language: Английский

Citations

151
Progression in translational research on spinal cord injury based on microenvironment imbalance DOI Creative Commons

Baoyou Fan,

Zhijian Wei,

Shiqing Feng

et al.

Bone Research, Journal Year: 2022, Volume and Issue: 10(1)

Published: April 8, 2022

Spinal cord injury (SCI) leads to loss of motor and sensory function below the level imposes a considerable burden on patients, families, society. Repair injured spinal has been recognized as global medical challenge for many years. Significant progress made in research pathological mechanism injury. In particular, with development gene regulation, cell sequencing, tracing technologies, in-depth explorations SCI microenvironment have become more feasible. However, translational studies related repair not yielded significant results. This review summarizes latest two aspects pathology: intraneuronal imbalance regenerative imbalance. We also strategies based imbalance, including medications, transplantation, exosomes, tissue engineering, reprogramming, rehabilitation. The current state future directions are discussed. combined, precise, multitemporal strategy repairing is potential direction.

Language: Английский

Citations

138
Mesenchymal stem cells-derived exosomes for drug delivery DOI Creative Commons
Yao Sun, Guoliang Liu, Kai Zhang

et al.

Stem Cell Research & Therapy, Journal Year: 2021, Volume and Issue: 12(1)

Published: Oct. 30, 2021

Abstract Exosomes are extracellular vesicles secreted by various cells, mainly composed of lipid bilayers without organelles. In recent years, an increasing number researchers have focused on the use exosomes for drug delivery. Targeted delivery in body is a promising method treating many refractory diseases such as tumors and Alzheimer's disease (AD). Finding suitable carrier has become popular research today. studies, mesenchymal stem cells (MSC-EXOs) been broadly researched due to their immune properties, tumor-homing elastic properties. While MSC-EXOs apparent advantages, some unresolved problems also exist. This article reviews studies delivery, summarizes characteristics MSC-EXOs, introduces primary production purification methods loading provide solutions existing suggestions future studies.

Language: Английский

Citations

127
Exosomes based strategies for brain drug delivery DOI
Fawad Ur Rehman, Yang Liu, Meng Zheng

et al.

Biomaterials, Journal Year: 2022, Volume and Issue: 293, P. 121949 - 121949

Published: Dec. 8, 2022

Language: Английский

Citations

120
Dental pulp stem cell-derived exosomes suppress M1 macrophage polarization through the ROS-MAPK-NFκB P65 signaling pathway after spinal cord injury DOI Creative Commons
Chao Liu, Fanqi Hu,

Genlong Jiao

et al.

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Feb. 2, 2022

Stem cell-derived exosomes have recently been regarded as potential drugs for treating spinal cord injury (SCI) by reducing reactive oxygen species (ROS) and suppressing M1 macrophage polarization. However, the roles of ROS in process polarization are not known. Herein, we demonstrated that can induce a concentration-dependent effect. through MAPK-NFκB P65 signaling pathway. Dental pulp stem cell (DPSC)-derived reduce ROS-MAPK-NFκB pathway SCI. This study suggested DPSC-derived might be drug Disruption cycle between also effective treatment secondary damage.

Language: Английский

Citations

116
Extracellular vesicles deposit PCNA to rejuvenate aged bone marrow–derived mesenchymal stem cells and slow age-related degeneration DOI
Qian Lei, Fei Gao, Teng Liu

et al.

Science Translational Medicine, Journal Year: 2021, Volume and Issue: 13(578)

Published: Jan. 27, 2021

Umbilical cord MSC–derived extracellular vesicles rejuvenate aged bone marrow MSCs and slow age-related degeneration in mice.

Language: Английский

Citations

115
Boosting extracellular vesicle secretion DOI
Lior Debbi, Shaowei Guo, Dina Safina

et al.

Biotechnology Advances, Journal Year: 2022, Volume and Issue: 59, P. 107983 - 107983

Published: May 17, 2022

Language: Английский

Citations

113
Stimulating Extracellular Vesicles Production from Engineered Tissues by Mechanical Forces DOI
Shaowei Guo, Lior Debbi,

Barak Zohar

et al.

Nano Letters, Journal Year: 2021, Volume and Issue: 21(6), P. 2497 - 2504

Published: March 12, 2021

Extracellular vesicles (EVs) have emerged as a promising strategy to promote tissue regeneration. However, overcoming the low EV production yield remains big challenge in translating EV-based therapies clinical practice. Current relies heavily on 2D cell culture, which is not only less physiologically relevant cells but also requires substantial medium and space. In this study, we engineered tissues seeded with stem from dental pulp or adipose tissues, skeletal muscle cells, significantly enhanced by applying mechanical stimuli, including flow stretching, bioreactors. Further mechanistic investigation revealed that process was mediated yes-associated protein (YAP) mechanosensitivity. EVs mechanically stimulated 3D scaffolds displayed superior capability inducing axonal sprouting than counterparts. Our results demonstrate promise of boost optimize their functional performance toward translation.

Language: Английский

Citations

104