Antidiabetic potency of glimepiride and naringin: an in silico and in vitro investigation DOI

Deepankar Rath,

Gurudutta Pattnaik, Biswakanth Kar

et al.

Journal of Biomolecular Structure and Dynamics, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Dec. 28, 2024

Glimepiride (GLM) is one of the potential antidiabetic drugs used in clinics for a long time. It currently combination with metformin along other drugs, but has shown various complications patients from long-term use. Thus, hypothesis to use lower dose GLM non-toxic class flavonoid, naringin (NARN), better therapy minimal side-effects. Initially, we assessed binding efficacy and NARN against nine putative target enzymes using AutoDock 4.2 software. We also analysed drug chemistry, drug-ability, cytotoxicity, as well performed molecular dynamic (MD) simulation at 100 ns individual states GROMACS-2022 Both candidates showed higher efficacy, especially AKT-serine/threonine kinase-1 (AKT1) enzyme (−11.85 kcal/mol), demonstrated stability compatibility AKT1 MD-simulation (based on RMSD, Rg, RMSF, H-bond plots) than form. The vitro cytotoxicity human embryonic kidney (HEK-293) cells suggested µg/mL (observed 80% cell viability) further study. Alpha-amylase, alpha-glucosidase, DPP-IV inhibition assays revealed that both inhibited up 60% concentration-dependent manner. At end, selecting (2:8 v/v ratio) 87% µg/mL. silico studies suggest investigated formulation could be diabetics.

Language: Английский

Chitosan and Its Nanoparticles: A Multifaceted Approach to Antibacterial Applications DOI Creative Commons
Emir Akdaşçi, Hatice Duman, Furkan Eker

et al.

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(2), P. 126 - 126

Published: Jan. 16, 2025

Chitosan, a multifaceted amino polysaccharide biopolymer derived from chitin, has extensive antibacterial efficacy against diverse pathogenic microorganisms, including both Gram-negative and Gram-positive bacteria, in addition to fungi. Over the course of last several decades, chitosan nanoparticles (NPs), which are polymeric bio-based, have garnered great deal interest as efficient agents. This is mostly due fact that they used wide variety applications, medical treatments, food, chemicals, agricultural products. Within context mechanism NPs, we present review provides an overview synthesis methods, novel procedures, compiles applications been developed field biomedicine. These include wound healing, drug delivery, dental treatment, water purification, agriculture, food preservation. In this, focus on mechanisms action factors determine activity its derivatives. conjunction with this line inquiry, researchers strongly urged concentrate their efforts developing ground-breaking NPs.

Language: Английский

Citations

2
Sensitive and on-Site Detection of Staphylococcus aureus Based on CRISPR/Cas 13a-Assisted Chemiluminescence Resonance Energy Transfer DOI
Xiaoqi Tao, Yue Li, Tian Tian

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(22), P. 9270 - 9277

Published: May 21, 2024

Developing a specific, sensitive, rapid, and on-site method for detecting pathogenic bacteria in food samples is critical to ensuring public safety. This article demonstrates CRISPR/Cas13a system chemiluminescence resonance energy transfer (CRET) (CRISPR/Cas 13a-assisted CRET)-based strategy sensitive detection of real samples. Once the hybrid double strand aptamerS. aureus-cRNA recognizes target model Staphylococcus aureus (S. aureus), released cRNA would bind with CRISPR/Cas 13a form complex cRNA-CRISPR/Cas 13a, which could cleave RNA molecule probe horseradish peroxidase (HRP) modified-gold nanoparticles (AuNPs) linked by (AuNPs-RNA-HRP), resulting an enhanced signal due CRET "OFF" phenomenon after introducing substrate luminol. The successfully detected S. drinking water milk limits 20 30 cfu/mL, respectively, within recovery 90.07–105.50%. Furthermore, integrating immunochromatographic test strip (ICTS), achieved as low 102 cfu/mL via smartphone, about 10 times lower than that previously reported AuNPs-based colorimetric ICTS, demonstrating convenient

Language: Английский

Citations

8
Fabrication of chitosan-coated ciprofloxacin drug nanocomposite for combating antibacterial and respiratory infection leading to sepsis DOI
Haoguang Li,

Zhiguo Su

Journal of materials research/Pratt's guide to venture capital sources, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Language: Английский

Citations

0
Development and characterization of biodegradable antibacterial hydrogels of xanthan gum for controlled ciprofloxacin release DOI

Siru Huang,

Susu An,

Perumal Ramesh Kannan

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142637 - 142637

Published: March 1, 2025

Language: Английский

Citations

0
Antidiabetic potency of glimepiride and naringin: an in silico and in vitro investigation DOI

Deepankar Rath,

Gurudutta Pattnaik, Biswakanth Kar

et al.

Journal of Biomolecular Structure and Dynamics, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Dec. 28, 2024

Glimepiride (GLM) is one of the potential antidiabetic drugs used in clinics for a long time. It currently combination with metformin along other drugs, but has shown various complications patients from long-term use. Thus, hypothesis to use lower dose GLM non-toxic class flavonoid, naringin (NARN), better therapy minimal side-effects. Initially, we assessed binding efficacy and NARN against nine putative target enzymes using AutoDock 4.2 software. We also analysed drug chemistry, drug-ability, cytotoxicity, as well performed molecular dynamic (MD) simulation at 100 ns individual states GROMACS-2022 Both candidates showed higher efficacy, especially AKT-serine/threonine kinase-1 (AKT1) enzyme (−11.85 kcal/mol), demonstrated stability compatibility AKT1 MD-simulation (based on RMSD, Rg, RMSF, H-bond plots) than form. The vitro cytotoxicity human embryonic kidney (HEK-293) cells suggested µg/mL (observed 80% cell viability) further study. Alpha-amylase, alpha-glucosidase, DPP-IV inhibition assays revealed that both inhibited up 60% concentration-dependent manner. At end, selecting (2:8 v/v ratio) 87% µg/mL. silico studies suggest investigated formulation could be diabetics.

Language: Английский

Citations

0