Recent Advances in Food Safety Detection: Split Aptamer-Based Biosensors Development and Potential Applications

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Ensuring food safety is a shared responsibility across the entire supply chain, encompassing manufacturers, processors, retailers, consumers, and regulatory bodies. However, traditional detection methods have several limitations, including slow processing times, high costs, limited sensitivity, susceptibility to false positives or negatives. These shortcomings underscore urgent need for faster, more accurate, cost-effective technologies. Aptamers aptasensors emerged as promising alternatives. offer advantages over recognition probes due their affinity specificity diverse targets. The enable rapid detection, cost reduction, shelf life extension, minimal batch-to-batch variability, making them highly suitable applications. Detecting small molecules such toxins, antibiotics, pesticides, contaminants, heavy metals remains challenging steric hindrance, nonspecific binding, reduced accuracy. Recent advancements in aptamer technology focused on pre- postmodifications enhance performance. One of most innovations development split aptamers. engineered aptamers, designed operate segments known improved flexibility binding specificity, effectively addressing challenges detecting small-sized This review examines evolution aptamers aptasensors, focusing application that are essential safety. It reported strategies modifying optimizing selected providing details developing approach address unique small-molecule detection. Additionally, recent its integration into aptasensor highlighted, showcasing how these revolutionizing hazards by overcoming limitations methods.

Language: Английский

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Revolutionizing Cancer Immunotherapy: Emerging Nanotechnology-Driven Drug Delivery Systems for Enhanced Therapeutic Efficacy

ACS Measurement Science Au, Journal Year: 2024, Volume and Issue: 5(1), P. 31 - 55

Published: Nov. 15, 2024

Cancer immunotherapy is an innovative way of treating cancer by stimulating individual cells to overcome cancer. Widespread biomedical studies were carried out with the aim exploring therapeutics, and this review spotlights some mechanisms in which it was developed, namely immune checkpoint inhibitors (E.G PD-1/PD-L1, CTLA-4), adoptive cell therapy (e.g., CAR T-cell therapy), vaccines. Although has shown clinical benefit a number types, including melanoma non-small-cell lung cancer, several challenges have dampened enthusiasm for approach, from differing patient response rates toxicities. Nanotechnology drug delivery systems must play role overcoming same. enables increased specificity controlled release, improved solubility bioavailability, can treat tumor specifically, localized at disease site decreases systemic toxicity. The also features advances construction lipid-based, polymeric, inorganic nanoparticles that improve stability allow cotherapeutic agents. Nanotechnology-based be used alone or combination assist improving response, gaining access microenvironment, biological barriers. Thus, nano-DDS both safe effective preclinical studies, ongoing trials they are capable increasing therapeutic index anticancer drugs. Lastly, discusses current regulatory issues advancing these technologies highlights importance further research devise appropriate methodology efficient functionalization nanotechnology individualized solutions treatment.

Language: Английский

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Interaction studies unveil potential binding sites on bovine serum albumin for gut metabolite trimethylamine n-oxide (TMAO)

BMC Chemistry, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 21, 2025

Trimethylamine-N-oxide (TMAO) is gut microbiota-derived metabolite, plays a critical role in human health and diseases such as metabolic, cardiovascular, colorectal cancer and, neurological disorders. Binding interactions between TMAO serum albumins are crucial to understand the impact of on disease mechanisms. However, detailed insights into interaction mechanisms, preferred binding locations, conformational changes BSA upon still unclear. interacts with albumin body thus, model study for TMAO-BSA conjugate presented support it. Decrease absorbance intensity protein metabolites reveals formation, while fluorescence spectroscopy indicate static quenching. Contact angle measurements further reveal hydrophilic nature complex, CD FTIR but structure remain intact. Computational studies, molecular docking, dynamics simulation MM/GBSA, confirm stable complex energy − 3.6 kcal/mol. These findings provide foundation understanding pharmacodynamics pharmacokinetics may aid developing strategies treating diseases, chronic kidney disorder where TMAO-serum implicated.

Language: Английский

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In-Silico Identification and Evaluation of Diosgenin as a Promising Phytomedicine Targeting MHC-I for Cancer Therapy

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Immunotherapeutic strategies targeting MHC-I have gained significant attention for combating cancer, a leading global health. However, the emergence of therapeutic resistance and manifestation adverse concerneffects associated with conventional treatment modalities underscore imperative innovative strategies. In current study, we conducted an “in silico”investigation to identify potential phytomedicines cancer (3AM8). Using AutodockTools software, 408 natural secondary polyphenols were screened against MHC-I, Diosgenin exhibiting highest affinity binding (−8.93 Kcal/mol). Pharmacokinetic profiling highest-ranking ligands elucidated their suitability subsequent development optimization. Molecular dynamics simulations, using Desmond software suite, demonstrated pronounced stability Diosgenin-MHC-I complex throughout 100-ns trajectory. Our findings suggest that holds promise as candidate therapeutics; however, further verifications by use extensive vitro” vivo”research is warranted substantiate its validity potential. This study highlights in upcoming trial regimens advances choices treating cancer.

Language: Английский

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Nanostructured aptasensors for ricin detection and tumor therapy: exploring aptamer-protein interactions and conformational stability in biological complexities

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143282 - 143282

Published: April 1, 2025

Language: Английский

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Aptamers capable of simultaneously identifying multiple targets and corresponding applications in medical diagnosis—A review

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143666 - 143666

Published: April 1, 2025

Language: Английский

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Multifaceted arsenal in SELEX nanomedicine

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 342, P. 103540 - 103540

Published: May 2, 2025

Language: Английский

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Aptamers: Design, Theory, and Applications to Diagnosis and Therapy for Diseases

MedComm, Journal Year: 2025, Volume and Issue: 6(5)

Published: May 1, 2025

ABSTRACT Single‐stranded DNA or RNA entities referred to as aptamers exhibit a strong affinity and specificity for attaching specific targets. Owing their special properties, which include simplicity of synthesis, low immunogenicity, adaptability in targeting variety substances, these synthetic oligonucleotides have garnered lot interest. The function can be altered by combining them with complementary “antidotes,” are antisense particular aptamer sequence. Antidotes play an important role several fields specifically the corresponding section aptamer. Nevertheless, even promising capabilities, creation antidotes regulate inhibit continues relatively unexamined field, constraining secure efficient application medical environments. review explores experimental methodologies creating antidotes, systematic design strategies managing aptamer‐based therapies, therapeutic efficacy counteracting disease biomarkers. Additionally, it highlights diagnostic applications biosensing imaging, offering alternative traditional antibodies. It also investigates progress, latest innovations, potential uses aptamer–antidote combinations. Its academic value lies bridging gap between theoretical practical applications, providing researchers clinicians comprehensive resource advance solutions medicine biotechnology.

Language: Английский

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Real-world application of molecular docking in drug discovery

Advances in pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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RECENT FOCUS IN NON-SELEX-COMPUTATIONAL APPROACH FOR DE NOVO APTAMER DESIGN: A MINI REVIEW

Analytical Biochemistry, Journal Year: 2024, Volume and Issue: 699, P. 115756 - 115756

Published: Dec. 26, 2024

Language: Английский

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