Development of highly sensitive one-pot ERA-CRISPR assays for on-site detection of CaMV35S promoter and NOS terminator in genetically modified crops DOI
Qianqian Liang, Xiaohui Wang,

Qingqing Xie

et al.

Food Control, Journal Year: 2024, Volume and Issue: unknown, P. 111049 - 111049

Published: Nov. 1, 2024

Language: Английский

Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolution DOI
Y Li, Ru Xu,

Fenglei Quan

et al.

EBioMedicine, Journal Year: 2025, Volume and Issue: 112, P. 105564 - 105564

Published: Jan. 24, 2025

Language: Английский

Citations

1
Droplet pairing-merging enabled digital RPA-CRISPR/Cas12a (DIMERIC) assay for rapid and precise quantification of Hepatitis B Virus DNA DOI
Dongyang Cai, Yifan Wang, Ziyi Zhang

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 276, P. 117256 - 117256

Published: Feb. 13, 2025

Language: Английский

Citations

1
Trends in developing one-pot CRISPR diagnostics strategies DOI
Lin Chen,

Menglu Hu,

Xiaoming Zhou

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Language: Английский

Citations

4
Rapid Detection of Spike Protein Receptor Binding Region of SARS-CoV-2 and Its Variants Using a Nanosheet Probe DOI
Jiacheng Yang, Jing Wang, Heng Zhang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Strategies for the rapid detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are critically needed due to conventional methods' limitations: narrow range, virus mutation-induced failure, time-consuming, and complex operations. Herein, we propose a method utilizing CuInP2S6 nanosheet probes Bio-Layer Interferometry (BLI) technology (5–10 min), noninvasive, broad-spectrum SARS-CoV-2 spike receptor binding domain (RBD) in human saliva. The nanoprobe exhibits higher affinity RBD compared most saliva proteins, allowing it be immobilized on BLI sensors easier protein elution. An eluent buffer containing Tween-20 salts was employed separate salivary proteins while retaining probes. This system can detect across broad spectrum low limit 25 ng/mL (S/N = 3) less than 10 min. To validate this system, experiments with pseudoviruses showed accurate identification RBD. Molecular dynamics simulations elucidated mechanism selective In conclusion, conceptual study based an situ strategy inorganic nanoprobes alongside techniques convenient, its variants. is anticipated inspire design implementation pathogens future.

Language: Английский

Citations

0
Engineered CRISPR/Cas Ribonucleoproteins for Enhanced Biosensing and Bioimaging DOI

Linxin Cao,

Zeyuan Wang, Chunyang Lei

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

CRISPR-Cas systems represent a highly programmable and precise nucleic acid-targeting platform, which has been strategically engineered as versatile toolkit for biosensing bioimaging applications. Nevertheless, their analytical performance is constrained by inherent functional activity limitations of natural CRISPR/Cas systems, underscoring the critical role molecular engineering in enhancing capabilities. This review comprehensively examines recent advancements ribonucleoproteins (RNPs) to enhance capabilities advanced detection cellular imaging. We explore innovative strategies developing enhanced RNPs, including Cas protein through mutagenesis fusion techniques, guide RNA via chemical structural modifications. Furthermore, we evaluate these RNPs' applications sensitive biomarker live-cell genomic DNA monitoring, while analyzing current challenges prospective developments RNP bioimaging.

Language: Английский

Citations

0
Palm-sized CRISPR sensing platform for on-site Mycoplasma pneumoniae detection DOI
Jiajun Li, Haobo Huang, Zerui Song

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117458 - 117458

Published: April 1, 2025

Language: Английский

Citations

0
Acoustofluidic precise manipulation: Recent advances in applications for micro/nano bioparticles DOI

Wanglu Li,

Zhihao Yao, Tongtong Ma

et al.

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 332, P. 103276 - 103276

Published: Aug. 12, 2024

Language: Английский

Citations

3
CRISPR-integrated metal-organic frameworks for biosensing applications: Recent advances and future perspective DOI Creative Commons
Babak Mikaeeli Kangarshahi,

Anahita Beigi,

Seyed Morteza Naghib

et al.

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: unknown, P. 100736 - 100736

Published: Jan. 1, 2025

Language: Английский

Citations

0
Some of Our Favorite Papers from the First 10 Years of ACS Sensors DOI Creative Commons
J. Justin Gooding

ACS Sensors, Journal Year: 2025, Volume and Issue: 10(1), P. 1 - 3

Published: Jan. 24, 2025

Language: Английский

Citations

0
A one-pot, one-step CRISPR-AapCas12b-based platform for sensitive and specific detection of Klebsiella pneumoniae DOI Creative Commons
Juan Zhou,

Zirong Wu,

Lei Yu

et al.

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: 48, P. 100797 - 100797

Published: April 29, 2025

Language: Английский

Citations

0