Environmental Science & Technology, Journal Year: 2013, Volume and Issue: 47(9), P. 4536 - 4543

Published: April 1, 2013

In the present study, a novel and efficient technology based on use of an oxidative enzyme was developed to perform continuous removal estrogenic compounds from polluted wastewaters. A 2 L enzymatic membrane reactor (EMR) successfully operated for 100 h with minimal requirements laccase transformation estrone (E1), 17β-estradiol (E2), 17α-ethinylestradiol (EE2)from both buffer solution real wastewater (filtered secondary effluent). When experiments were performed at high low concentrations target compounds, 4 mg/L μg/L, not only yields (80-100%) but also outstanding reduction estrogenicity (about 84-95%) attained. EMR applied treatment municipal wastewaters environmental different (0.29-1.52 ng/L), excellent results achieved indicating efficiency potential system. second goal this study relied identification products elucidate catalytic mechanism estrogens' by laccase. The formation dimers trimers E1, E2, EE2, as well decomposition E2 into E1 laccase-catalyzed treatment, has been demonstrated liquid chromatography atmospheric pressure chemical ionization (LC-APCI) analysis confirmed determination accurate masses through electrospray time-of-flight mass spectrometry (LC-ESI-TOF). Dimeric EE2 found even when operating concentrations. Moreover, reaction pathways proposed.

Language: Английский