Quantification of Biomolecular Condensate Volume Reveals Network Swelling and Dissolution Regimes during Phase Transition DOI Creative Commons
Iris B. A. Smokers, Evan Spruijt

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 363 - 373

Published: Dec. 2, 2024

Accurate determination of biomolecular condensate volume reveals that destabilization condensates can lead to either swelling or shrinking condensates, giving fundamental insights into the regulation cellular condensates. Determination and coacervate protocells is essential investigate their precise composition impact on (bio)chemical reactions are localized inside It not a straightforward task, as have tiny volumes, highly viscous, prone wetting. Here, we examine different strategies determine introduce two new methods, with which volumes 1 μL less (volume fraction 0.4%) be determined standard deviation 0.03 μL. Using these show depends degree physical cross-linking. These observations supported by Flory–Huggins theory profound effects in cell biology.

Language: Английский

Martini3-IDP: improved Martini 3 force field for disordered proteins DOI Creative Commons
Liguo Wang, Christopher Brasnett, Luís Borges-Araújo

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 24, 2025

Coarse-grained (CG) molecular dynamics (MD) is widely used for the efficient simulation of intrinsically disordered proteins (IDPs). The Martini model, one most popular CG force fields in biomolecular simulation, was reported to yield too compact IDP conformations, limiting its applications. Addressing this, we optimized bonded parameters based on fitting reference simulations a diverse set IDPs at atomistic resolution, resulting Martini3-based protein model coined Martini3-IDP. This leads expanded greatly improving reproduction experimentally measured radii gyration. Moreover, contrary ad-hoc fixes scaling protein-protein or protein-water interactions, Martini3-IDP keeps overall interaction balance underlying 3. To validate that, perform comprehensive testing including full-length multidomain proteins, IDP-lipid membrane binding and IDP-small molecule binding, confirming ability successfully capture complex interplay between components. Finally, recently emerging concept condensate, through liquid-liquid phase separation, also reproduced by number both homotypic heterotypic systems. With improved expand simulate processes involving environments, spatio-temporal scales inaccessible with all-atom models. Here, authors introduce Martini3-IDP, refined that addresses prior over-compact structures. Validated across systems, it captures interactions condensates.

Language: Английский

Citations

2
Scanning probe microscopy elucidates gelation and rejuvenation of biomolecular condensates DOI Creative Commons
Aida Naghilou, Oskar Armbruster, Alireza Mashaghi

et al.

Cell Reports Physical Science, Journal Year: 2025, Volume and Issue: unknown, P. 102430 - 102430

Published: Feb. 1, 2025

Language: Английский

Citations

1
Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles DOI
Agustín Mangiarotti, Mina Aleksanyan, Macarena Siri

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(23)

Published: April 6, 2024

Abstract Interactions between membranes and biomolecular condensates can give rise to complex phenomena such as wetting transitions, mutual remodeling, endocytosis. In this study, light‐triggered manipulation of condensate engulfment is demonstrated using giant vesicles containing photoswitchable lipids. UV irradiation increases the membrane area, which be stored in nanotubes. When contact with a droplet, light triggers rapid endocytosis, reverted by blue light. The affinity protein‐rich reversibility processes quantified from confocal microscopy images. degree photo‐induced engulfment, whether partial or complete, depends on vesicle excess area relative sizes condensates. Theoretical estimates suggest that utilizing light‐induced increase vesicle‐condensate adhesion interface energetically more favorable than energy gain folding into invaginations tubes. overall findings demonstrate membrane‐condensate interactions easily quickly modulated via light, providing versatile system for building platforms control cellular events design intelligent drug delivery systems cell repair.

Language: Английский

Citations

8
Interplay between membranes and biomolecular condensates in the regulation of membrane-associated cellular processes DOI Creative Commons
Nari Kim, H.K. Yun, Ho‐Jin Lee

et al.

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Abstract Liquid‒liquid phase separation (LLPS) has emerged as a key mechanism for organizing cellular spaces independent of membranes. Biomolecular condensates, which assemble through LLPS, exhibit distinctive liquid droplet-like behavior and can exchange constituents with their surroundings. The regulation condensate phases, including transitions from state to gel or irreversible aggregates, is important physiological functions controlling pathological progression, observed in neurodegenerative diseases cancer. While early studies on biomolecular condensates focused primarily those fluidic environments such the cytosol, recent discoveries have revealed existence close proximity to, on, even comprising aim this review provide an overview properties membrane-associated context biological relation

Language: Английский

Citations

6
Elastin-like polypeptide coacervates as reversibly triggerable compartments for synthetic cells DOI Creative Commons
Chang Chen, Ketan A. Ganar, Robbert J. de Haas

et al.

Communications Chemistry, Journal Year: 2024, Volume and Issue: 7(1)

Published: Sept. 4, 2024

Language: Английский

Citations

5
Membrane nanotubes transform into double-membrane sheets at condensate droplets DOI Creative Commons
Ziliang Zhao, Vahid Satarifard, Reinhard Lipowsky

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(26)

Published: June 20, 2024

Cellular membranes exhibit a multitude of highly curved morphologies such as buds, nanotubes, cisterna-like sheets defining the outlines organelles. Here, we mimic cell compartmentation using an aqueous two-phase system dextran and poly(ethylene glycol) encapsulated in giant vesicles. Upon osmotic deflation, vesicle membrane forms which undergo surprising morphological transformations at liquid–liquid interfaces inside At these interfaces, nanotubes transform into double-membrane (DMS) connected to mother via short necks. Using super-resolution (stimulated emission depletion) microscopy theoretical considerations, construct morphology diagram predicting tube-to-sheet transformation, is driven by decrease free energy. Nanotube knots can prohibit transformation blocking water influx tubes. Because both DMSs are frequently formed cellular membranes, understanding formation between provides insight origin evolution

Language: Английский

Citations

4
Functional Biomaterials Derived from Protein Liquid–Liquid Phase Separation and Liquid‐to‐Solid Transition DOI Creative Commons
Tianchen Li, Dea Prianka Ayu Ilhamsyah,

Benjamin C.U. Tai

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Protein phase transitions play a vital role in both cellular functions and pathogenesis. Dispersed proteins can undergo liquid-liquid separation to form condensates, process that is reversible highly regulated within cells. The formation physicochemical properties of these such as composition, viscosity, multiphase miscibility, are precisely modulated fulfill specific biological functions. However, protein condensates further liquid-to-solid state, forming β-sheet-rich aggregates may disrupt function lead diseases. While this phenomenon crucial for processes has significant implications neurodegenerative diseases, the behavior naturally derived or engineered polypeptides also presents opportunities developing high-performance, multifunctional materials at various scales. Additionally, unique molecular recruitment capabilities inspire innovative advancements biomaterial design applications drug discovery, delivery, biosynthesis. This work highlights recent progress understanding mechanisms underlying behavior, particularly how it responds internal changes external physical stimuli. Furthermore, fabrication from diverse sources through controlled demonstrated.

Language: Английский

Citations

0
The Role of Membrane-Biomolecular Condensate Interactions in Stress DOI Creative Commons
Konstantin Kutashev, Panagiotis N. Moschou

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100810 - 100810

Published: March 1, 2025

Language: Английский

Citations

0
Interaction of native and aggregated albumin with DMPC bilayers DOI

Brigitte Magdalena Merino,

Rosa Bartucci, Rita Guzzi

et al.

Biophysical Chemistry, Journal Year: 2025, Volume and Issue: 322, P. 107431 - 107431

Published: March 14, 2025

Language: Английский

Citations

0
Lipid packing and cholesterol content regulate membrane wetting and remodeling by biomolecular condensates DOI Creative Commons
Agustín Mangiarotti, Elias Sabri,

Kita Valerie Schmidt

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 20, 2025

Abstract Biomolecular condensates play a central role in cellular processes by interacting with membranes driving wetting transitions and inducing mutual remodeling. While are known to locally alter membrane properties such as lipid packing hydration, it remains unclear how composition phase state turn affect condensate affinity. Here, we show that is not only the itself, but rather degree of determines affinity for membranes. Increasing chain length, saturation, or cholesterol content, enhances packing, thereby decreasing interaction. This regulatory mechanism consistent across various condensate-membrane systems, highlighting critical interface. In addition, protein adsorption promotes extensive remodeling, including formation tubes double-membrane sheets. Our findings reveal which fine-tunes wetting, its potential impact on functions organelle interactions.

Language: Английский

Citations

0