Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(23)
Published: April 6, 2024
Abstract
Interactions
between
membranes
and
biomolecular
condensates
can
give
rise
to
complex
phenomena
such
as
wetting
transitions,
mutual
remodeling,
endocytosis.
In
this
study,
light‐triggered
manipulation
of
condensate
engulfment
is
demonstrated
using
giant
vesicles
containing
photoswitchable
lipids.
UV
irradiation
increases
the
membrane
area,
which
be
stored
in
nanotubes.
When
contact
with
a
droplet,
light
triggers
rapid
endocytosis,
reverted
by
blue
light.
The
affinity
protein‐rich
reversibility
processes
quantified
from
confocal
microscopy
images.
degree
photo‐induced
engulfment,
whether
partial
or
complete,
depends
on
vesicle
excess
area
relative
sizes
condensates.
Theoretical
estimates
suggest
that
utilizing
light‐induced
increase
vesicle‐condensate
adhesion
interface
energetically
more
favorable
than
energy
gain
folding
into
invaginations
tubes.
overall
findings
demonstrate
membrane‐condensate
interactions
easily
quickly
modulated
via
light,
providing
versatile
system
for
building
platforms
control
cellular
events
design
intelligent
drug
delivery
systems
cell
repair.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(42)
Published: Aug. 31, 2023
Cellular
membranes,
including
the
plasma
and
endosome
are
barriers
to
outside
proteins.
Various
vehicles
have
been
devised
deliver
proteins
across
membrane,
but
in
many
cases,
payload
gets
trapped
endosome.
Here
we
designed
a
photo-responsive
phase-separating
fluorescent
molecule
(PPFM)
with
molecular
weight
of
666.8
daltons.
The
PPFM
compound
condensates
as
droplets
aqueous
solution
by
liquid-liquid
phase
separation
(LLPS),
which
disintegrate
upon
photoirradiation
405
nm
light-emitting
diode
(LED)
lamp
within
20
min
or
laser
3
min.
coacervates
recruit
wide
range
peptides
them
into
mammalian
cells.
Photolysis
disperses
from
cytosolic
space.
Altogether,
type
small
molecules
that
separating
discovered;
their
can
serve
transmembrane
for
intracellular
delivery
proteins,
whereas
photo
illumination
triggers
distribution
payload.
Small Methods,
Journal Year:
2023,
Volume and Issue:
7(12)
Published: April 17, 2023
Abstract
In
the
extant
lifeforms,
self‐sustaining
behaviors
refer
to
various
well‐organized
biochemical
reactions
in
spatial
confinement,
which
rely
on
compartmentalization
integrate
and
coordinate
molecularly
crowded
intracellular
environment
complicated
reaction
networks
living/synthetic
cells.
Therefore,
biological
phenomenon
of
has
become
an
essential
theme
field
synthetic
cell
engineering.
Recent
progress
state‐of‐the‐art
cells
indicated
that
multi‐compartmentalized
should
be
developed
obtain
more
advanced
structures
functions.
Herein,
two
ways
developing
hierarchical
systems,
namely
interior
(organelles)
integration
communities
(synthetic
tissues),
are
summarized.
Examples
provided
for
different
construction
strategies
employed
above‐mentioned
engineering
ways,
including
spontaneous
vesicles,
host–guest
nesting,
phase
separation
mediated
multiphase,
adhesion‐mediated
assembly,
programmed
arrays,
3D
printing.
Apart
from
exhibiting
functions,
also
applied
as
biomimetic
materials.
Finally,
key
challenges
future
directions
regarding
development
systems
summarized;
these
expected
lay
foundation
creation
a
“living”
well
provide
larger
platform
new
materials
future.
Annual Review of Condensed Matter Physics,
Journal Year:
2023,
Volume and Issue:
15(1), P. 237 - 261
Published: Dec. 7, 2023
Living
cells
are
spatially
organized
by
compartments
that
can
nucleate,
grow,
and
dissolve.
Compartmentalization
emerge
phase
separation,
leading
to
the
formation
of
droplets
in
cell's
nucleo-
or
cytoplasm,
also
called
biomolecular
condensates.
Such
organize
biochemistry
cell
providing
specific
chemical
environments
space
time.
These
provide
transient
environments,
suggesting
relevance
nonequilibrium
physics
as
a
key
unraveling
underlying
physicochemical
principles
biological
functions
living
cells.
In
this
review,
we
highlight
coarse-grained
approaches
capture
chemically
active
emulsions
model
for
condensates
orchestrating
processes.
We
discuss
dynamics
single
molecules
material
properties
their
cell.
Finally,
propose
wetting,
prewetting,
surface
transitions
possibility
intracellular
surfaces
control
condensates,
membranes,
exert
mechanical
forces.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(23)
Published: April 6, 2024
Abstract
Interactions
between
membranes
and
biomolecular
condensates
can
give
rise
to
complex
phenomena
such
as
wetting
transitions,
mutual
remodeling,
endocytosis.
In
this
study,
light‐triggered
manipulation
of
condensate
engulfment
is
demonstrated
using
giant
vesicles
containing
photoswitchable
lipids.
UV
irradiation
increases
the
membrane
area,
which
be
stored
in
nanotubes.
When
contact
with
a
droplet,
light
triggers
rapid
endocytosis,
reverted
by
blue
light.
The
affinity
protein‐rich
reversibility
processes
quantified
from
confocal
microscopy
images.
degree
photo‐induced
engulfment,
whether
partial
or
complete,
depends
on
vesicle
excess
area
relative
sizes
condensates.
Theoretical
estimates
suggest
that
utilizing
light‐induced
increase
vesicle‐condensate
adhesion
interface
energetically
more
favorable
than
energy
gain
folding
into
invaginations
tubes.
overall
findings
demonstrate
membrane‐condensate
interactions
easily
quickly
modulated
via
light,
providing
versatile
system
for
building
platforms
control
cellular
events
design
intelligent
drug
delivery
systems
cell
repair.
