An Enzyme‐Engineered Coppery Nanozyme for High‐Efficiency Mild Photothermal/Chemodynamic/Starvation Therapy Through Self‐Reinforcing Cancer Energy Metabolism Regulation

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(22)

Published: Feb. 5, 2024

Abstract Photothermal therapy (PTT) has a great prospect in further improving tumor therapeutic outcomes, whereas its efficiency is restrained by low light penetration, excessive heat damage to normal tissues, up‐regulated shock proteins (HSPs), and limited effect of single treatment. Herein, an enzyme‐engineered coppery nanozyme based on dendritic mesoporous carbon nanosphere as the cornerstone load with glucose oxidase (GOx) followed modification hyaluronic acid constructed. Density functional theory calculations indicate that obtained exhibits peroxidase glutathione mimicking activities improve hydroxyl radicals (•OH) production. Furthermore, both generation •OH production GOx‐induced energy supply blockade can reduce HSPs expression enhance mild PTT (η = 34.9 %) upon irradiation 1064 nm laser, turn, accelerate catalytic processes for more •OH. Last but not least, introduction copper induce lipoylated protein dihydrolipoamide S‐acetyltransferase aggregation cause cellular cuproptosis. Due synergy multiple therapies, inhibition rate reach 93.4%. Overall, this work provides effective strategy potential treatment basis synergistic therapies.

Language: Английский

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Single-Atom-Based Nanoenzyme in Tissue Repair

ACS Nano, Journal Year: 2024, Volume and Issue: 18(20), P. 12639 - 12671

Published: May 8, 2024

Since the discovery of ferromagnetic nanoparticles Fe3O4 that exhibit enzyme-like activity in 2007, research on nanoenzymes has made significant progress. With in-depth study various and rapid development related nanotechnology, have emerged as a promising alternative to natural enzymes. Within nanozymes, there is category metal-based single-atom nanozymes been rapidly developed due low cast, convenient preparation, long storage, less immunogenicity, especially higher efficiency. More importantly, possess capacity scavenge reactive oxygen species through mechanisms, which beneficial tissue repair process. Herein, this paper systemically highlights types metal their catalytic recent applications repair. The existing challenges are identified prospects future composed metallic nanomaterials proposed. We hope review will illuminate potential repair, encouraging sequential clinical translation.

Language: Английский

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Chitosan‐Stabilized PtAu Nanoparticles with Multienzyme‐Like Activity for Mixed Bacteria Infection Wound Healing and Insights into Its Antibacterial Mechanism

Small Structures, Journal Year: 2024, Volume and Issue: 5(6)

Published: March 25, 2024

Traditional antibacterial agents are often observed to be ineffective because bacteria evolved strains with greater antibiotic resistance. Here, vigorous chitosan‐stabilized PtAu nanoparticles (CSPA) multienzyme‐like activity successfully fabricated, which serve an effective artificial nanozyme enhance for mixed bacterial infection wound treatment. Ultrasmall size CSPA exhibits excellent hydrophilicity and biocompatibility, possesses strong oxidase‐ peroxidase‐like generating a substantial amount of ROS (, 1 O 2 , ·OH) cause oxidative damage bacteria, also demonstrates nicotinamide adenine dinucleotide dehydrogenase‐like disrupting the respiratory chains, subsequently impedes adenosine triphosphate production. favorable broad‐spectrum at very low concentrations, prevents resistance, completely inhibits biofilm formation. Antibacterial Mechanism by transcriptomics is further revealed that can induce stress, hinder energy metabolism, disrupt synthesis function cell walls membranes. In vivo, population site promotes healing in rats. This study introduces novel approach, providing important insight into mechanism nanozymes promoting advancement nanocatalytic materials biomedical applications.

Language: Английский

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Hypoxia-tropic delivery of nanozymes targeting transferrin receptor 1 for nasopharyngeal carcinoma radiotherapy sensitization

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 21, 2025

Nasopharyngeal carcinoma (NPC), a malignancy highly prevalent in East and Southeast Asia, is primarily treated with radiotherapy (RT). However, hypoxia-induced radioresistance presents significant challenge. Nanozymes, nanomaterials catalase-like activity, have emerged as promising strategy for radiosensitization by converting elevated hydrogen peroxide the tumor microenvironment into oxygen. Despite their potential, effectively targeting hypoxic lesions has been difficult. Here, we identify transferrin receptor 1 (TfR1) an upregulated target NPC, its expression levels positively correlated hypoxia. Human heavy-chain ferritin, specific ligand of TfR1, selectively recognizes NPC preclinical models. Based on these findings, design hypoxia-targeted nanozyme loading platinum nanoparticles ferritin. This exhibits enhanced activity alleviates hypoxia xenografts. When combined RT, single injection significantly inhibits growth prolongs mouse survival, outperforming sodium glycididazole, clinically used radiosensitizer. In summary, our findings highlight TfR1 accessible cell surface lesions. The holds promise enhancing therapeutic effectiveness RT through situ oxygen-generation mechanism. Transferrin shown to be nasopharyngeal (NPC). Here authors report that can targeted using ferritin nanozymes.

Language: Английский

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Pt Single-Atom-Functionalized 2D Al-TCPP MOF Nanosheets for Enhanced Photodynamic Antimicrobial Therapy

Acta Physico-Chimica Sinica, Journal Year: 2025, Volume and Issue: unknown, P. 100046 - 100046

Published: Jan. 1, 2025

Language: Английский

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