Zwitterionic Polyelectrolyte Complex Vesicles Assembled from Homopoly(2-Oxazoline)s as Enzyme Catalytic Nanoreactors for Potent Anti-Tumor Efficiency DOI

Hepeng Wang,

Guojing Zhang,

Min Lin

et al.

Langmuir, Journal Year: 2024, Volume and Issue: 40(37), P. 19423 - 19429

Published: July 31, 2024

Enzymes are known for their remarkable catalytic efficiency across a wide range of applications. Here, we present novel and convenient nanoreactor platform based on zwitterionic polyelectrolyte complex vesicles (PCVs), assembled from oppositely charged homopoly(2-oxazoline)s, facilitating enzyme immobilization. We show enhancements in activity stability by encapsulation lipase as model enzyme. Even the temperature rises, performance remains robust. Further, structural characteristics PCVs, including hollow architecture semipermeable membranes, endow them with unique advantages cascade reactions involving glucose oxidase (GOx) horseradish peroxidase (HRP). A decline is shown when enzymes individually loaded subsequently mixed, contrast to coloaded GOx–HRP–PCV group. demonstrate that vesicle structures establish confined environments where precise enzyme–substrate interactions facilitate enhanced efficiency. In addition, nanoreactors exhibit excellent biocompatibility efficient anti-tumor activity, which hold significant promise biomedical applications within enzyme-based technologies.

Language: Английский

Peptide-Mediated Liquid-Liquid Phase Separation and Biomolecular Condensates DOI

Guangle Li,

Chengqian Yuan, Xuehai Yan

et al.

Soft Matter, Journal Year: 2025, Volume and Issue: 21(10), P. 1781 - 1812

Published: Jan. 1, 2025

Peptide-mediated liquid–liquid phase separation (LLPS) underpins the formation of dynamic biomolecular condensates, regulated by diverse molecular interactions, and highlights potential applications in drug delivery synthetic biology.

Language: Английский

Citations

0
3D-printed Multifunctional Bilayer Scaffold with Sustained Release of Apoptotic Extracellular Vesicles and Antibacterial Coacervates for Enhanced Wound Healing DOI
Linli Jiang, Jia Dong, Minwen Jiang

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: 318, P. 123196 - 123196

Published: Feb. 15, 2025

Language: Английский

Citations

0
RNA condensates as platforms for prebiotic chemistry DOI Creative Commons
Samuel Hauf, Ryô Nakamura, Barbara Cellini

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract The emergence of the first catalytically active biopolymers remains a scientific mystery. Some abiotic chemistries for formation precursor molecules to — such as nucleotides and amino acids are known. However, polymerization usually results in short polymers only few units length. This is too meaningful information storage or catalytic activity, limitation known Flory Length Problem. Additionally, biocatalysts presumably had low activity because they were generated by random polymerization. Therefore, enrichment substrates catalysts seems necessary significant rates catalysis occur. Could RNA phase separation be solution these challenges? Our experimental evidence demonstrates that at acidic pH, RNAs ( < 20 nt) readily phase-separate into condensed enriched with longer fragments, primarily through phosphate backbone protonation. These condensates stably compartmentalize DNA species without rapid flux genetic material, maintaining their identity over extended periods even absence membranes. In addition, concentrates ions critical folding along small organic molecules, phospholipids, peptides, ribozymes, large proteins. Beyond enriching diverse components, function microreactors dual capabilities. They physically enhance reaction concentrating reactants within confined space simultaneously act inherent directly facilitate chemical transformations. also support ribozyme enzymatic activity. Collectively, findings suggest may have played crucial role life’s origins providing spatial compartmentalization, biopolymer enrichment, particularly potentially species.

Language: Английский

Citations

0
Core-shell coacervates formed from DNA nanostars DOI Creative Commons

Karuna Skipper,

Shelley F. J. Wickham

Published: March 12, 2024

Phase-separating DNA coacervates have important potential as model protocells, uniquely combining tuneable material properties with programmable biomolecular interactions. However, the membrane-free nature of leads to instability and heterogeneity, limiting their ability mimic cell behaviour. Here, we develop multi-layered coacervate ‘droplets’ composed nanostars, where nanostars distinct sequences form both cytosolic core membrane-like shell. Nanostar were first explored understand how structural changes in geometry, valency, interaction strength affect phase-separation temperature, size, stability, permeability. We show that when pairs self-assemble same solution, order determines or shell destination, while proportion surfactant link two populations morphology. For 3 different demonstrate a range morphologies. Membrane-like systems, fully encloses core, if difference temperatures is greater than 7C 16-25% nanostars. Core-shell droplets more cell-like, well-defined stability over time, permeability controlled by properties. Furthermore, droplet size membrane thickness adjusting thermal annealing rate during assembly. These techniques provide diverse library suitable be used protocells predictable mono-dispersity, thickness, nanostar will open new avenues for programming dynamic cell-like behaviour simplified systems combine molecular circuits.

Language: Английский

Citations

3
Sustainable leather alternatives: High-performance and dyeable bio-based materials from fungal chitin and tannic acid DOI

Fengteng Zhang,

Gentao Fu,

Hai Liu

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 348, P. 122800 - 122800

Published: Sept. 24, 2024

Language: Английский

Citations

3
Benchmark of Coacervate Formation and Mechanism Exploration Using the Martini Force Field DOI

Rongrong Zou,

Yiwei Wang, Xiu Zhang

et al.

Journal of Chemical Theory and Computation, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Peptide-based coacervates are crucial for drug delivery due to their biocompatibility, versatility, high loading capacity, and cell penetration rates; however, stability mechanism phase behavior not fully understood. Additionally, although Martini is one of the most famous force fields capable describing coacervate formation with molecular details, a comprehensive benchmark its accuracy has been conducted. This research utilized 3.0 field machine learning algorithms explore representative peptide-based coacervates, including those composed polyaspartate (PAsp)/polyarginine (PArg), rmfp-1, sticker-and-spacer small molecules, HBpep molecules. We identified key driving forces such as Coulomb, cation–π, π–π interactions established three criteria determining in simulations. The results also indicate that while accurately captures trends, it tends underestimate Coulomb overestimate interactions. What more, our study on encapsulation derivative suggested loaded drugs were distributed surfaces clusters, awaiting experimental validation. employs simulation enhance understanding mechanisms benchmarking 3.0, thereby providing fundamental insights future investigations.

Language: Английский

Citations

0
Biomineralization‐Inspired Membranization Toward Structural Enhancement of Coacervate Community DOI Creative Commons
Chunyu Zhao, Xiaoliang Wang,

Lianning Li

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

Abstract The design and assembly of protocell models that can mimic the features functions life present a significant research challenge with potential for far‐reaching impact. Inspired by natural phenomenon microbe‐induced mineralization, way is developed to induce spontaneous formation mineralized membrane on surface coacervate droplets utilizing Fe 3+ ions. In particular, effect ions microstructure at molecular level dissected combining theoretical experimental approaches. reversible process be regulated redox reactions involving 2+ /Fe within coacervate. not only enhances stability prevents aggregation coalescence, but also allows adjacent together. membranized assemblages retain inherent properties biomolecule sequestration enzyme catalysis, demonstrate excellent resistance high temperatures pressures as well good over 30 days. This study will offer new platform coacervate‐based life‐like biomimetic systems, enhance understanding interactions underlying various biological phenomena level.

Language: Английский

Citations

0
Tumor Microenvironment-Responsive Lipid Peroxidation Amplifier: Harnessing Ferroptosis Resistance to Devastate the Ferroptosis Defense System DOI

Youhui Zeng,

Jinfeng Yang, Zhen Gu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Amplification of lipid peroxidation with tumor specificity represents a new avenue to boost ferroptosis-mediated anticancer therapeutics but remains challenging. Herein, we proposed metal-phenolic-network (MPN)-coated nanohybrid as microenvironment-responsive amplifier, consisting reactive oxygen species generator MPN, glutathione (GSH) scavenger GSH-P, and peroxidase 4 (GPX4) mRNA gene silencing sequence. The protective MPN shell this amplifier can be specifically disintegrated by acidic adenosine triphosphate (ATP)-rich microenvironments induce oxidative stress through the dual disruption redox homeostasis (Fenton-catalytic accumulation GSH depletion). Furthermore, stress-induced upregulation ferroptosis resistance-related apurinic/apyrimidinic endonuclease 1 (APE1) is further ingeniously employed an amplification element prompt release (AP) site-embedded GPX4 sequence which downregulate level. Based on tandem depletion substrate GPX4, defense system GPX4/GSH heavily devastated enable for effectively improving efficiency. We expect strategy expanded other important regulatory proteins provide mechanism study therapy.

Language: Английский

Citations

0
Synthesis of Mesoporous Catechin Nanoparticles as Biocompatible Drug-Free Antibacterial Mesoformulation DOI
Runfeng Lin, Gaoyang Li, Qianfeng He

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(39), P. 26983 - 26993

Published: Sept. 18, 2024

While polyphenolic substances stand as excellent antibacterial agents, their antimicrobial properties rely on the auxiliary support of micro-/nanostructures. Despite offering a novel avenue for enhancing polymer performance, controllable fabrication mesoporous polymeric nanomaterials encounters significant challenges due to intricate intermolecular forces. In this article, catechin nanoparticles have been successfully fabricated using balanced multivariate interaction approach. The harmonization water-ethanol ratio and ionic strength effectively balances forces hydrogen bonding π-π stacking, facilitating controlled assembly mesostructures. exhibit uniform spherical structure (∼100 nm), open mesopores with diameter ∼15 nm, high surface area ∼106 m

Language: Английский

Citations

3
Fluidic Membrane-Bound Protocells Enabling Versatile Assembly of Functional Nanomaterials for Biomedical Applications DOI
Baihao You, Chia‐Hung Chen

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 30, 2024

The development of membrane-bound protocells, which process cascade biochemical reactions in distinct microcompartments, marks a significant advancement soft systems. However, many synthesized protocells with cell membrane-like structures are prone to rupturing biological environments and challenging functionalize, limiting their biomedical applications. In this study, we explore the liquid-liquid phase separation tannic acid (TA) polyethylene glycol (PEG) form coacervate droplets. Upon introducing polyvinylpyrrolidone (PVP) molecules, dense hydrogen bonding network spontaneously forms at surfaces droplets, resulting robust fluidic (FMPs). These can be flexibly postfunctionalized incorporate functional nanomaterials via electrostatic attraction, enabling design for To demonstrate this, nanozymes (Pt/CeO

Language: Английский

Citations

2