Biomimetic peptide self-assembly for functional materials

Nature Reviews Chemistry, Journal Year: 2020, Volume and Issue: 4(11), P. 615 - 634

Published: Sept. 15, 2020

Language: Английский

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Peptide-based coacervates as biomimetic protocells

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 50(6), P. 3690 - 3705

Published: Jan. 1, 2021

This tutorial review describes molecular design principles for peptides and peptide derivatives undergoing phase separation highlights the potential of resulting coacervate protocells.

Language: Английский

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Tunable multiphase dynamics of arginine and lysine liquid condensates

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Sept. 15, 2020

Liquid phase separation into two or more coexisting phases has emerged as a new paradigm for understanding subcellular organization, prebiotic life, and the origins of disease. The design principles underlying biomolecular have potential to drive development novel liquid-based organelles therapeutics, however, an how individual molecules contribute emergent material properties, approaches directly manipulate dynamics are lacking. Here, using microrheology, we demonstrate that droplets poly-arginine coassembled with mono/polynucleotides approximately 100 fold greater viscosity than comparable lysine droplets, both which can be finer tuned by polymer length. We find these amino acid-level differences formation immiscible tunable kinetics further exploited trigger controlled release droplet components. Together, this work provides mechanism leveraging sequence-level components in order regulate multiphase coexistence.

Language: Английский

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Coacervates as models of membraneless organelles

Current Opinion in Colloid & Interface Science, Journal Year: 2020, Volume and Issue: 52, P. 101416 - 101416

Published: Dec. 30, 2020

Coacervates are condensed liquid-like droplets, usually formed with oppositely charged polymeric molecules. They have been studied extensively in colloid and interface science for their remarkable material properties. The liquid–liquid phase separation underlying coacervate formation also plays an important role the of various membraneless organelles (MLOs) that found many living cells. Therefore, there is increasing interest to use well-characterized coacervates as vitro models mimic specific aspects MLOs. Here, we review five – physical chemical properties, hierarchical organization, uptake selectivity, dynamics, maturation particular discuss how useful better understand these

Language: Английский

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Sequence-encoded and composition-dependent protein-RNA interactions control multiphasic condensate morphologies

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Feb. 8, 2021

Multivalent protein-protein and protein-RNA interactions are the drivers of biological phase separation. Biomolecular condensates typically contain a dense network multiple proteins RNAs, their competing molecular play key roles in regulating condensate composition structure. Employing ternary system comprising prion-like polypeptide (PLP), arginine-rich (RRP), RNA, we show that competition between PLP RNA for single shared partner, RRP, leads to RNA-induced demixing PLP-RRP into stable coexisting phases-homotypic heterotypic RRP-RNA condensates. The morphology these biphasic (non-engulfing/ partial engulfing/ complete engulfing) is determined by RNA-to-RRP stoichiometry hierarchy intermolecular interactions, providing glimpse broad range multiphasic patterns accessible Our findings provide minimal set physical rules govern spatial organization multicomponent biomolecular

Language: Английский

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Liquid–Liquid Phase Separation by Intrinsically Disordered Protein Regions of Viruses: Roles in Viral Life Cycle and Control of Virus–Host Interactions

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(23), P. 9045 - 9045

Published: Nov. 28, 2020

Intrinsically disordered proteins (IDPs) are unable to adopt a unique 3D structure under physiological conditions and thus exist as highly dynamic conformational ensembles. IDPs ubiquitous widely spread in the protein realm. In last decade, compelling experimental evidence has been gathered, pointing ability of intrinsically regions (IDRs) undergo liquid–liquid phase separation (LLPS), phenomenon driving formation membrane-less organelles (MLOs). These biological condensates play critical role spatio-temporal organization cell, where they exert multitude key functions, ranging from transcriptional regulation silencing control signal transduction networks. After introducing LLPS, we herein survey available data on LLPS by IDPs/IDRs viral origin discuss their functional implications. We distinguish associated with replication trafficking components, LLPS-mediated interference viruses host cell functions. emerging plant virus interfere MLOs propose that bacteriophages can bacterial well. conclude discussing how could be targeted treat separation-associated diseases, including infections.

Language: Английский

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Polyelectrolyte Complex Coacervates: Recent Developments and New Frontiers

Annual Review of Condensed Matter Physics, Journal Year: 2020, Volume and Issue: 12(1), P. 155 - 176

Published: Nov. 24, 2020

Polyelectrolyte complex coacervates represent a wide class of materials with applications ranging from coatings and adhesives to pharmaceutical technologies. They also underpin multiple biological processes, which are only now beginning be deciphered. The means by molecular-scale architecture propagates into macroscopic structure, thermodynamics, dynamics in is central concern physics, chemistry, biology, science. How does polyion charge sequence dictate thermodynamic behavior? one tailor rheology or interfacial tension using macromolecular architecture? What emergent functionality polymer has consequences? Recent developments coacervate science shed light on many these issues raise exciting new challenges for the close integration theory, simulations, experiment.

Language: Английский

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ATP‐Responsive and ATP‐Fueled Self‐Assembling Systems and Materials

Advanced Materials, Journal Year: 2020, Volume and Issue: 32(42)

Published: Sept. 2, 2020

Adenosine triphosphate (ATP) is a central metabolite that plays an indispensable role in various cellular processes, from energy supply to cell-to-cell signaling. Nature has developed sophisticated strategies use the stored ATP for many metabolic and non-equilibrium sense bind biological The variations concentrations one organelle another, extracellular intracellular environments, normal cells cancer are motivation designing ATP-triggered ATP-fueled systems materials, because they show great potential applications by using as trigger or chemical fuel. Over last decade, been emerging attractive co-assembling component man-made stimuli-responsive well fuel-driven active materials. Herein, current advances concepts self-assemblies materials discussed, shedding light on highlighting future developments. By bringing together of different domains, supramolecular chemistry DNA nanoscience, equilibrium self-assembly, fundamental sciences applications, aim cross-fertilize approaches with ultimate bring synthetic ATP-dependent closer living systems.

Language: Английский

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Surface Electrostatics Govern the Emulsion Stability of Biomolecular Condensates

Nano Letters, Journal Year: 2022, Volume and Issue: 22(2), P. 612 - 621

Published: Jan. 10, 2022

Liquid–liquid phase separation underlies the formation of biological condensates. Physically, such systems are microemulsions that in general have a propensity to fuse and coalesce; however, many condensates persist as independent droplets test tube inside cells. This stability is crucial for their function, but physicochemical mechanisms control emulsion remain poorly understood. Here, by combining single-condensate zeta potential measurements, optical microscopy, tweezer experiments, multiscale molecular modeling, we investigate how nanoscale forces sustain impact against fusion. By comparing peptide–RNA (PR25:PolyU) proteinaceous (FUS) condensates, show higher condensate surface charge correlates with lower fusion propensity. Moreover, measurements single potentials reveal can constitute classically stable emulsions. Taken together, these results highlight role passive stabilization protecting biomolecular coalescence.

Language: Английский

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Engineering synthetic biomolecular condensates

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(7), P. 466 - 480

Published: April 17, 2023

Language: Английский

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