Gut,
Journal Year:
2011,
Volume and Issue:
60(9), P. 1214 - 1223
Published: May 14, 2011
Abstract
Neuropeptides
have
been
reported
to
regulate
progenitor
proliferation
and
neurogenesis
in
the
central
nervous
system.
However,
these
studies
typically
conducted
using
pharmacological
agents
ex
vivo
preparations,
in
evidence
for
their
developmental
function
is
generally
lacking.
Recent
scRNA-Seq
identified
multiple
neuropeptides
receptors
as
being
selectively
expressed
neurogenic
progenitors
of
embryonic
mouse
human
retina.
This
includes
Sstr2,
whose
ligand
somatostatin
transiently
by
immature
retinal
ganglion
cells.
By
analyzing
explants
treated
with
selective
ligands
that
target
receptors,
we
found
Sstr2-dependent
signaling
induces
a
dose-dependent
inhibition
photoreceptor
generation
while
increasing
relative
fraction
primary
These
effects
were
confirmed
analysis
abolished
Sstr2-deficient
retinas.
Although
no
changes
or
precursors
observed
retinas
vivo,
demonstrated
accelerated
differentiation
progenitors.
We
conclude
Sstr2
may
act
negatively
combination
other
cell-derived
secreted
factors
such
Shh,
although
dispensable
normal
development.
