CD73 alleviates GSDMD‐mediated microglia pyroptosis in spinal cord injury through PI3K/AKT/Foxo1 signaling DOI Creative Commons
Shun Xu, Jin Wang, Junjie Zhong

et al.

Clinical and Translational Medicine, Journal Year: 2020, Volume and Issue: 11(1)

Published: Dec. 31, 2020

Abstract Background Neuroinflammation‐induced secondary injury is an important cause of sustained progression spinal cord injury. Inflammatory programmed cell death pyroptosis executed by the pore‐forming protein gasdermin D (GSDMD) essential step neuroinflammation. However, it unclear whether CD73, a widely accepted immunosuppressive molecule, can inhibit via mediating GSDMD. Methods C57BL/6J CD73 deficient mice and wild‐type mice, Lipopolysaccharide (LPS)‐induced primary microglia BV2 cells were respectively used to illustrate effect on in vivo vitro. A combination molecular histological methods was performed assess explore mechanism both Results We have shown evidence for suppresses activation NLRP3 inflammasome complexes reduce maturation GSDMD, leading decreased microglia. Further analysis reveals that adenosine‐A 2B adenosine receptor‐PI3K‐AKT‐Foxo1 cascade possible regulation. Importantly, we determine inhibits expression GSDMD at transcriptional level through Foxo1. What's more, confirm accumulation HIF‐1α promotes overexpression after (SCI), increased turn upregulates HIF‐1α, eventually forming positive feedback regulatory loop. Conclusion Our data reveal novel function pyroptosis, suggesting unique therapeutic opportunity mitigating disease process SCI.

Language: Английский

Pyroptosis: mechanisms and diseases DOI Creative Commons

Pian Yu,

Xu Zhang, Nian Liu

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)

Published: March 29, 2021

Abstract Currently, pyroptosis has received more and attention because of its association with innate immunity disease. The research scope expanded the discovery gasdermin family. A great deal evidence shows that can affect development tumors. relationship between tumors is diverse in different tissues genetic backgrounds. In this review, we provide basic knowledge pyroptosis, explain tumors, focus on significance tumor treatment. addition, further summarize possibility as a potential treatment strategy describe side effects radiotherapy chemotherapy caused by pyroptosis. brief, double-edged sword for rational use dual effect will help us explore formation ideas patients to develop new drugs based

Language: Английский

Citations

1568
Nickel: Human Health and Environmental Toxicology DOI Open Access
Giuseppe Genchi, Alessia Carocci, Graziantonio Lauria

et al.

International Journal of Environmental Research and Public Health, Journal Year: 2020, Volume and Issue: 17(3), P. 679 - 679

Published: Jan. 21, 2020

Nickel is a transition element extensively distributed in the environment, air, water, and soil. It may derive from natural sources anthropogenic activity. Although nickel ubiquitous its functional role as trace for animals human beings has not been yet recognized. Environmental pollution be due to industry, use of liquid solid fuels, well municipal industrial waste. contact can cause variety side effects on health, such allergy, cardiovascular kidney diseases, lung fibrosis, nasal cancer. molecular mechanisms nickel-induced toxicity are clear, mitochondrial dysfunctions oxidative stress thought have primary crucial this metal. Recently, researchers, trying characterize capability induce cancer, found out that epigenetic alterations induced by exposure perturb genome. The purpose review describe chemical features toxicity. Furthermore, attention focused strategies remove phytoremediation phytomining.

Language: Английский

Citations

1155
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) DOI Open Access
Andrea Cossarizza, Hyun‐Dong Chang, Andreas Radbruch

et al.

European Journal of Immunology, Journal Year: 2019, Volume and Issue: 49(10), P. 1457 - 1973

Published: Oct. 1, 2019

These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed. Furthermore, tips, tricks pitfalls avoid, written peer-reviewed by leading experts field, making this an essential research companion.

Language: Английский

Citations

876
Regulated cell death (RCD) in cancer: key pathways and targeted therapies DOI Creative Commons
Peng Fu,

Minru Liao,

Rui Qin

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: Aug. 13, 2022

Regulated cell death (RCD), also well-known as programmed (PCD), refers to the form of that can be regulated by a variety biomacromolecules, which is distinctive from accidental (ACD). Accumulating evidence has revealed RCD subroutines are key features tumorigenesis, may ultimately lead establishment different potential therapeutic strategies. Hitherto, targeting with pharmacological small-molecule compounds been emerging promising avenue, rapidly progressed in many types human cancers. Thus, this review, we focus on summarizing not only apoptotic and autophagy-dependent signaling pathways, but crucial pathways other subroutines, including necroptosis, pyroptosis, ferroptosis, parthanatos, entosis, NETosis lysosome-dependent (LCD) cancer. Moreover, further discuss current situation several improve cancer treatment, such single-target, dual or multiple-target compounds, drug combinations, some new strategies would together shed light future directions attack vulnerabilities drugs for purposes.

Language: Английский

Citations

487
The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer DOI Creative Commons
Mingxia Jiang, Ling Qi, Lisha Li

et al.

Cell Death Discovery, Journal Year: 2020, Volume and Issue: 6(1)

Published: Oct. 28, 2020

Abstract Apoptosis has long been recognized as a mechanism that kills the cancer cells by cytotoxic drugs. In recent years, studies have proved pyroptosis can also shrink tumors and inhibit proliferation. Both apoptosis are caspase-dependent programmed cell death pathways. Cysteinyl aspartate specific proteinase-3 (Caspase-3) is common key protein in pathways, when activated, expression level of tumor suppressor gene Gasdermin E (GSDME) determines death. When GSDME highly expressed, active caspase-3 cuts it releases N-terminal domain to punch holes membrane, resulting swelling, rupture, low, will lead classical death, which apoptosis. More interestingly, researchers found be located upstream caspase-3, connecting extrinsic, intrinsic apoptotic Then, promoting activation, forming self-amplifying feed-forward loop. GSDME-mediated correlated with side effects chemotherapy anti-tumor immunity. This article mainly reviews caspase-3/GSDME signal pathway switch between cancer, provide new strategies targets for treatment.

Language: Английский

Citations

471
The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling DOI Creative Commons
Guozhi Bi, Min Su, Nan Li

et al.

Cell, Journal Year: 2021, Volume and Issue: 184(13), P. 3528 - 3541.e12

Published: May 12, 2021

Language: Английский

Citations

434
Nanocatalysts‐Augmented and Photothermal‐Enhanced Tumor‐Specific Sequential Nanocatalytic Therapy in Both NIR‐I and NIR‐II Biowindows DOI
Wei Feng, Xiuguo Han,

Rong-Yan Wang

et al.

Advanced Materials, Journal Year: 2018, Volume and Issue: 31(5)

Published: Dec. 9, 2018

Abstract The tumor microenvironment (TME) has been increasingly recognized as a crucial contributor to tumorigenesis. Based on the unique TME for achieving tumor‐specific therapy, here novel concept of photothermal‐enhanced sequential nanocatalytic therapy in both NIR‐I and NIR‐II biowindows is proposed, which innovatively changes condition Fenton reaction production highly efficient hydroxyl radicals (•OH) consequently suppressing growth. Evidence suggests that glucose plays vital role powering cancer progression. Encouraged by oxidation gluconic acid H 2 O oxidase (GOD), an Fe 3 4 /GOD‐functionalized polypyrrole (PPy)‐based composite nanocatalyst constructed achieve diagnostic imaging‐guided, photothermal‐enhanced, TME‐specific therapy. consumption intratumoral GOD leads situ elevation level, integrated component then catalyzes into toxic •OH efficiently induce cancer‐cell death. Importantly, high photothermal‐conversion efficiency (66.4% biowindow) PPy elevates local temperature substaintially accelerate improve disproportionation degree enhancing nanocatalytic‐therapeutic efficacy, successfully achieves remarkable synergistic anticancer outcome with minimal side effects.

Language: Английский

Citations

405
Ferroptosis occurs through an osmotic mechanism and propagates independently of cell rupture DOI
Michelle Riegman,

Liran Sagie,

Chen Galed

et al.

Nature Cell Biology, Journal Year: 2020, Volume and Issue: 22(9), P. 1042 - 1048

Published: Aug. 31, 2020

Language: Английский

Citations

357
A novel defined pyroptosis-related gene signature for predicting the prognosis of ovarian cancer DOI Creative Commons

Ying Ye,

Qinjin Dai, Hongbo Qi

et al.

Cell Death Discovery, Journal Year: 2021, Volume and Issue: 7(1)

Published: April 7, 2021

Abstract Ovarian cancer (OC) is a highly malignant gynaecological tumour that has very poor prognosis. Pyroptosis been demonstrated in recent years to be an inflammatory form of programmed cell death. However, the expression pyroptosis-related genes OC and their correlations with prognosis remain unclear. In this study, we identified 31 pyroptosis regulators were differentially expressed between normal ovarian tissues. Based on these (DEGs), all cases could divided into two subtypes. The prognostic value each gene for survival was evaluated construct multigene signature using Cancer Genome Atlas (TCGA) cohort. By applying least absolute shrinkage selection operator (LASSO) Cox regression method, 7-gene built classified patients TCGA cohort low- or high-risk group. low-risk group showed significantly higher possibilities than those ( P < 0.001). Utilizing median risk score from cohort, Gene Expression Omnibus (GEO) subgroups, had increased overall (OS) time = 0.014). Combined clinical characteristics, found independent factor predicting OS patients. ontology (GO) Kyoto Encylopedia Genes Genomes (KEGG) analyses indicated immune-related enriched immune status decreased conclusion, play important roles immunity can used predict OCs.

Language: Английский

Citations

353
Neuronal Cell Death Mechanisms in Major Neurodegenerative Diseases DOI Open Access
Hao Ran,

Hui-Yun Chang,

Tzu‐Kang Sang

et al.

International Journal of Molecular Sciences, Journal Year: 2018, Volume and Issue: 19(10), P. 3082 - 3082

Published: Oct. 9, 2018

Neuronal cell death in the central nervous system has always been a challenging process to decipher. In normal physiological conditions, neuronal is restricted adult brain, even aged individuals. However, pathological conditions of various neurodegenerative diseases, and shrinkage specific region brain represent fundamental feature across different diseases. this review, we will briefly go through general pathways describe evidence for context individual common discussing our current understanding by connecting with renowned pathogenic proteins, including Tau, amyloid-beta, alpha-synuclein, huntingtin TDP-43.

Language: Английский

Citations

347