The epithelial-mesenchymal transition and the cytoskeleton in bioengineered systems

Cell Communication and Signaling, Journal Year: 2021, Volume and Issue: 19(1)

Published: March 10, 2021

Abstract The epithelial-mesenchymal transition (EMT) is intrinsically linked to alterations of the intracellular cytoskeleton and extracellular matrix. After EMT, cells acquire an elongated morphology with front/back polarity, which can be attributed actin-driven protrusion formation as well gain vimentin expression. Consequently, deform remodel surrounding matrix in order facilitate local invasion. In this review, we highlight recent bioengineering approaches elucidate EMT functional changes cytoskeleton. First, review transitions between multicellular clusters dispersed individuals on planar surfaces, often exhibit coordinated behaviors driven by leader EMT. Second, consider role vimentin, probed at subcellular length scales within confined spaces. Third, discuss topographical patterning via a contact guidance like mechanism. Finally, address how disorganize disseminate 3D These new technologies enable controlled physical microenvironments higher-resolution spatiotemporal measurements single cell level. closing, future directions for field outstanding questions regarding human cancer progression.

Language: Английский

---

Tissue mechanics in stem cell fate, development, and cancer

Developmental Cell, Journal Year: 2021, Volume and Issue: 56(13), P. 1833 - 1847

Published: June 8, 2021

Language: Английский

---

Force spectroscopy of single cells using atomic force microscopy

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: Sept. 23, 2021

Language: Английский

---

Biomolecular actuators for genetically selective acoustic manipulation of cells

Science Advances, Journal Year: 2023, Volume and Issue: 9(8)

Published: Feb. 22, 2023

The ability to physically manipulate specific cells is critical for the fields of biomedicine, synthetic biology, and living materials. Ultrasound has with high spatiotemporal precision via acoustic radiation force (ARF). However, because most have similar properties, this capability disconnected from cellular genetic programs. Here, we show that gas vesicles (GVs)-a unique class gas-filled protein nanostructures-can serve as genetically encodable actuators selective manipulation. Because their lower density higher compressibility relative water, GVs experience strong ARF opposite polarity other When expressed inside cells, invert cells' contrast amplify magnitude ARF, allowing be selectively manipulated sound waves based on genotype. provide a direct link between gene expression acoustomechanical actuation, opening paradigm control in broad range contexts.

Language: Английский

---

Adherens junctions as molecular regulators of emergent tissue mechanics

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 25(4), P. 252 - 269

Published: Dec. 13, 2023

Language: Английский

---