ODEP-based Robotic System for Manipulation and In-Flow Analysis of Primary Cells DOI Creative Commons
Joanna Filippi, Paola Casti, Valentina Lacconi

et al.

Cyborg and Bionic Systems, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 1, 2025

The presence of cellular defects multifactorial nature can be hard to characterize accurately and early due the complex interplay genetic, environmental, lifestyle factors. With this study, by bridging optically-induced dielectrophoresis (ODEP), microfluidics, live-cell imaging, machine learning, we provide ground for devising a robotic micromanipulation analysis system single-cell phenotyping. Cells under influence nonuniform electric fields generated via ODEP recorded measured. induced responses obtained time-variant stimulation reflect cells' chemical, morphological, structural characteristics in an automated, flexible, label-free manner. By complementing electrokinetic fingerprint cell centroid motion with data on dynamics electro-deformation orientation, show that subtle differences at level elucidated. Specifically, here, demonstrate, first time, ability combined ODEP-based automatic platform discriminate between primary endometrial stromal cells from fertile patients disrupted receptivity/selectivity equilibrium. When multiple were considered patient level, performance achieved average accuracy 98%. Single-cell micro-operation systems may find more general application clinical diagnosis management pathological alterations level.

Language: Английский

Revolutionizing the structural design and determination of covalent–organic frameworks: principles, methods, and techniques DOI
Yikuan Liu, Xiaona Liu, An Su

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 53(1), P. 502 - 544

Published: Dec. 15, 2023

Covalent organic frameworks (COFs) represent an important class of crystalline porous materials with designable structures and functions. The interconnected monomers, featuring pre-designed symmetries connectivities, dictate the COFs, endowing them high thermal chemical stability, large surface area, tunable micropores. Furthermore, by utilizing pre-functionalization or post-synthetic functionalization strategies, COFs can acquire multifunctionalities, leading to their versatile applications in gas separation/storage, catalysis, optoelectronic devices. Our review provides a comprehensive account latest advancements principles, methods, techniques for structural design determination COFs. These cutting-edge approaches enable rational precise elucidation COF structures, addressing fundamental physicochemical challenges associated host-guest interactions, topological transformations, network interpenetration, defect-mediated catalysis.

Language: Английский

Citations

47
Multivalent 9-O-Acetylated-sialic acid glycoclusters as potent inhibitors for SARS-CoV-2 infection DOI Creative Commons

Simon J. L. Petitjean,

Wenzhang Chen,

Melanie Koehler

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: May 10, 2022

Abstract The recent emergence of highly transmissible SARS-CoV-2 variants illustrates the urgent need to better understand molecular details virus binding its host cell and develop anti-viral strategies. While many studies focused on role angiotensin-converting enzyme 2 receptor in infection, others suggest important attachment factors such as glycans. Here, we use atomic force microscopy study these early events with focus sialic acids (SA). We show that binds specifically 9- O -acetylated-SA a moderate affinity, supporting an factor during landing surfaces. For therapeutic purposes based this finding, have designed novel blocking molecules various topologies carrying controlled number SA residues, enhancing affinity through multivalent effect. Inhibition assays AcSA-derived glycoclusters are potent inhibitors infectivity, offering new perspectives treatment infection.

Language: Английский

Citations

54
Study on microscopic physical and chemical properties of biomass materials by AFM DOI Creative Commons
Zhichao Lou, Yao Zhang, Yanjun Li

et al.

Journal of Materials Research and Technology, Journal Year: 2023, Volume and Issue: 24, P. 10005 - 10026

Published: May 1, 2023

Biomass is created by living things like plants, animals, and microbes. It has the characteristics of abundant reserves, renewable, biodegradable environmentally friendly. Research on biomass materials grown almost exponentially over last few decades. Despite current characterization techniques, it still difficult to simultaneously characterize materials' morphology mechanics at nanoscale level. Atomic force microscopy (AFM) offers a special benefit get beyond this restriction. AFM provides high-resolution images materials. Moreover, contact between item enables be detected without harming sample while sensing real-time mechanical topographic properties surface material. The ability probes interact with in mild environments (including normal temperature, pressure or biomimetic liquids) great significance for studying microscopic This paper reviews basic principles AFM, application progress field research micromechanical materials, adhesion, intermolecular interactions, etc.), conjunction FTIR RAMAN. In conclusion, unique method not only us grasp structure more comprehensively, but also essential insights understand interfacial interactions molecular level, which useful exploring contributing crucial practical material properties, processing bionic design.

Language: Английский

Citations

41
Towards glycan foldamers and programmable assemblies DOI

Surusch Djalali,

Nishu Yadav,

Martina Delbianco

et al.

Nature Reviews Materials, Journal Year: 2024, Volume and Issue: 9(3), P. 190 - 201

Published: Feb. 5, 2024

Language: Английский

Citations

16
Single-Molecule Investigation of the Binding Interface Stability of SARS-CoV-2 Variants with ACE2 DOI Creative Commons
Ankita Ray,

Tran Thi Minh Thu,

Rita dos Santos Natividade

et al.

ACS Nanoscience Au, Journal Year: 2024, Volume and Issue: 4(2), P. 136 - 145

Published: March 8, 2024

The SARS-CoV-2 pandemic spurred numerous research endeavors to comprehend the virus and mitigate its global severity. Understanding binding interface between human receptors is pivotal these efforts paramount curbing infection transmission. Here we employ atomic force microscopy steered molecular dynamics simulation explore receptor domain (RBD) variants angiotensin-converting enzyme 2 (ACE2), examining impact of mutations at key residues upon affinity. Our results show that Omicron Delta possess strengthened affinity in comparison Mu variant. Further, using sera from individuals either vaccinated or with acquired immunity following strain infection, assess variant RBD/ACE2 complex formation. Single-molecule spectroscopy analysis suggests vaccination before may provide stronger protection across variants. These underscore need monitor antigenic changes order continue developing innovative effective abrogation strategies.

Language: Английский

Citations

9
Probing Single-Cell Adhesion Kinetics and Nanomechanical Force with Surface Plasmon Resonance Imaging DOI
Dehong Yang, Xiaoyin Liu, Jinbiao Ma

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Single cell adhesion plays a significant role in numerous physiological and pathological processes. Real-time imaging quantification of single kinetics corresponding cell–substrate mechanical interaction forces are crucial for elucidating the cellular mechanisms involved tissue formation, immune responses, cancer metastasis. Here, we present development plasmonic-based nanomechanical sensing system (PNMSi) real-time measurement associated with plasmonic tracking monitoring interactions accompanying nanoscale fluctuations. Both slow binding dynamic processes were tracked analyzed thermodynamic model to determine kinetic parameters quantity forces. To demonstrate capabilities PNMSi platform, examined across four different surface modifications, obvious alterations observed, influenced by charges interfacial hydrophilicity. Additionally, investigated changes cells during cytoskeleton modification, revealing cross-linking-induced changes. Furthermore, application capability system, profiling primary tumor metastatic was explored, observed interaction. The platform facilitates high-throughput high sensitivity serves as promising identifying biomarkers metastasis screening potential therapeutic agents.

Language: Английский

Citations

1
Atomic force microscopy combined with microfluidics for label-free sorting and automated nanomechanics of circulating tumor cells in liquid biopsy DOI

Xiaoqun Qi,

Sen Lin, Mi Li

et al.

Nanoscale, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This work combines deep learning image recognition-assisted atomic force microscopy with contraction–expansion microfluidics to achieve label-free sorting and automated nanomechanics of circulating tumor cells for cancer liquid biopsy.

Language: Английский

Citations

1
Single-Molecule Biophysics: Probing Biological Processes at the Nanoscale DOI
Afam Uzorka

Biophysical Reviews and Letters, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 17

Published: March 29, 2025

Single-molecule biophysics has become a ground-breaking field that enables scientists to precisely study biological processes at the nanoscale. This review examines most current developments and uses of single-molecule methods for studying biomolecular interactions, DNA mechanics, protein folding, cellular dynamics, such as fluorescence microscopy force spectroscopy. The behavior functions individual biomolecules within intricate settings are better understood by researchers through biomolecules. Additionally, machines, development molecular motors, creation synthetic have all been made possible intriguing applications in biology. incorporation into these fields creates new opportunities comprehending basic utilizing engineering wide range biotechnology medical applications. More fundamental secrets life will be revealed this subject develops, opening door discoveries across scientific fields.

Language: Английский

Citations

1
Tumor Biomechanics Alters Metastatic Dissemination of Triple Negative Breast Cancer via Rewiring Fatty Acid Metabolism DOI Creative Commons
Elysse C. Filipe,

Sipiththa Velayuthar,

Ashleigh M. Philp

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(23)

Published: April 11, 2024

Abstract In recent decades, the role of tumor biomechanics on cancer cell behavior at primary site has been increasingly appreciated. However, effect latter stages metastatic cascade, such as seeding secondary sites and outgrowth remains underappreciated. This work sought to address this in context triple negative breast (TNBC), a type known aggressively disseminate all disease progression. Using mechanically tuneable model systems, mimicking range stiffness's typically found within tumors, it is that, contrary expectations, cells exposed softer microenvironments are more able colonize tissues. It shown that heightened survival driven by enhanced metabolism fatty acids TNBC microenvironments. demonstrated uncoupling cellular mechanosensing through integrin β1 blocking antibody effectively causes stiff primed behave like their soft counterparts, both vitro vivo. first show may be contributing changes outcome imprinting greater metabolic flexibility conferring discrete advantages.

Language: Английский

Citations

8
Factors influencing initial bacterial adhesion to antifouling surfaces studied by single-cell force spectroscopy DOI Creative Commons

Tal Duanis‐Assaf,

Meital Reches

iScience, Journal Year: 2024, Volume and Issue: 27(2), P. 108803 - 108803

Published: Jan. 6, 2024

Biofilm formation, a major concern for healthcare systems, is initiated when bacteria adhere to surfaces. Escherichia coli adhesion mediated by appendages, including type-1 fimbriae and curli amyloid fibers. Antifouling surfaces prevent the of combat biofilm formation. Here, we used single-cell force-spectroscopy study interaction between E. glass or two antifouling surfaces: tripeptide DOPA-Phe(4F)-Phe(4F)-OMe poly(ethylene glycol) polymer-brush. Our results indicate that both antifoulants significantly deter initial adhesion. By using mutant strains expressing no amyloids, studied mechanism. suggest different via separate mechanisms. Finally, show some much better than others, illustrating how variability bacterial cultures affects emphasize additional at level can enhance our understanding adhesion, thus leading novel technologies.

Language: Английский

Citations

7