The type IV pilus protein PilU functions as a PilT-dependent retraction ATPase DOI Creative Commons
David W. Adams, Jorge M. Pereira,

Candice Stoudmann

et al.

PLoS Genetics, Journal Year: 2019, Volume and Issue: 15(9), P. e1008393 - e1008393

Published: Sept. 16, 2019

Type IV pili are dynamic cell surface appendages found throughout the bacteria. The ability of these structures to undergo repetitive cycles extension and retraction underpins their crucial roles in adhesion, motility natural competence for transformation. In best-studied systems a dedicated ATPase PilT powers pilus retraction. Curiously, second presumed PilU is often encoded immediately downstream pilT. However, despite presence two potential ATPases, pilT deletions lead total loss function, raising question why fails take over. Here, using DNA-uptake mannose-sensitive haemagglutinin (MSHA) Vibrio cholerae as model systems, we show that inactivated variants, defective either ATP-binding or hydrolysis, have unexpected intermediate phenotypes PilU-dependent. addition demonstrating can function bona fide ATPase, go on make surprising discovery functions exclusively PilT-dependent manner identify naturally occurring pandemic V. variant renders essential function. Finally, Pseudomonas aeruginosa also providing evidence functional coupling between could be widespread mechanism optimal

Language: Английский

Type IV pili: dynamics, biophysics and functional consequences DOI
Lisa Craig, Katrina T. Forest, Berenike Maier

et al.

Nature Reviews Microbiology, Journal Year: 2019, Volume and Issue: 17(7), P. 429 - 440

Published: April 15, 2019

Language: Английский

Citations

402
Bacterial motility: machinery and mechanisms DOI
Navish Wadhwa, Howard C. Berg

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 20(3), P. 161 - 173

Published: Sept. 21, 2021

Language: Английский

Citations

325
Tree of motility – A proposed history of motility systems in the tree of life DOI Creative Commons
Makoto Miyata, Robert Robinson, Taro Q.P. Uyeda

et al.

Genes to Cells, Journal Year: 2020, Volume and Issue: 25(1), P. 6 - 21

Published: Jan. 1, 2020

Abstract Motility often plays a decisive role in the survival of species. Five systems motility have been studied depth: those propelled by bacterial flagella, eukaryotic actin polymerization and motor proteins myosin, kinesin dynein. However, many organisms exhibit surprisingly diverse motilities, advances genomics, molecular biology imaging showed that motilities inherently independent mechanisms. This makes defining breadth nontrivial, because novel may be driven unknown Here, we classify known based on unique classes movement‐producing protein architectures. Based this criterion, current total stands at 18 types. In perspective, discuss these modes relative to latest phylogenetic Tree Life propose history motility. During ~4 billion years since emergence life, arose Bacteria with flagella pili, Archaea archaella. Newer became possible Eukarya changes cell envelope. Presence or absence peptidoglycan layer, acquisition robust membrane dynamics, enlargement cells environmental opportunities likely provided context for (co)evolution types

Language: Английский

Citations

159
Quorum Sensing Enhances Direct Interspecies Electron Transfer in Anaerobic Methane Production DOI
Shunan Zhao,

Shaoqing Zhu,

Suo Liu

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(6), P. 2891 - 2901

Published: Feb. 3, 2024

Direct interspecies electron transfer (DIET) provides an innovative way to achieve efficient methanogenesis, and this study proposes a new approach upregulate the DIET pathway by enhancing quorum sensing (QS). Based on long-term reactor performance, QS enhancement achieved more vigorous methanogenesis with 98.7% COD removal efficiency. In control system, failure occurred at accumulated acetate of 7420 mg COD/L lowered pH 6.04, much lower 41.9% was observed. The significant in QS-enhancing system supported higher expression conductive pili c-Cyts cytochrome secretion-related genes, resulting 12.7- 10.3-fold improvements. Moreover, also improved energy production capability, increase F-type V/A-type ATPase 6.3- 4.2-fold, effect probably provided for nanowires secretion. From perspective community structure, increased abundance Methanosaeta Geobacter from 54.3 17.6% 63.0 33.8%, respectively. Furthermore, genes involved carbon dioxide reduction alcohol dehydrogenation 0.6- 7.1-fold, Taken together, indicates positive effects chemicals stimulate advances understanding environments such as anaerobic digesters sediments.

Language: Английский

Citations

33
Syntrophic growth with direct interspecies electron transfer between pili-free Geobacter species DOI Open Access
Xing Liu,

Shiyan Zhuo,

Christopher Rensing

et al.

The ISME Journal, Journal Year: 2018, Volume and Issue: 12(9), P. 2142 - 2151

Published: June 6, 2018

Language: Английский

Citations

117
The electrifying physiology of Geobacter bacteria, 30 years on DOI
Gemma Reguera,

Kazem Kashefi

Advances in microbial physiology/Advances in Microbial Physiology, Journal Year: 2019, Volume and Issue: unknown, P. 1 - 96

Published: Jan. 1, 2019

Language: Английский

Citations

117
DNA Uptake by Type IV Filaments DOI Creative Commons
Kurt H. Piepenbrink

Frontiers in Molecular Biosciences, Journal Year: 2019, Volume and Issue: 6

Published: Feb. 5, 2019

Bacterial uptake of DNA through type IV filaments is an essential component natural competence in numerous gram-positive and gram-negative species. Recent advances the field have broadened our understanding structures used to take up extracellular DNA. Here, we review seminal experiments literature describing binding by pili, pili flp Micrococcus luteus; collectively referred here as filaments. We compare current state on mechanisms for these three appendage systems describe mechanistic both DNA-binding DNA-uptake versatile molecular machines.

Language: Английский

Citations

113
Propulsive nanomachines: the convergent evolution of archaella, flagella and cilia DOI Open Access
Morgan Beeby, Josie L. Ferreira, Patrick Tripp

et al.

FEMS Microbiology Reviews, Journal Year: 2020, Volume and Issue: 44(3), P. 253 - 304

Published: March 6, 2020

ABSTRACT Echoing the repeated convergent evolution of flight and vision in large eukaryotes, propulsive swimming motility has evolved independently microbes each three domains life. Filamentous appendages – archaella Archaea, flagella Bacteria cilia Eukaryotes wave, whip or rotate to propel microbes, overcoming diffusion enabling colonization new environments. The implementations nanomachines are distinct, however: rotate, while beat wave; assemble at their tips, base; use ATP for motility, ion-motive force. These underlying differences reflect tinkering required evolve a molecular machine, which pre-existing machines appropriate contexts were iteratively co-opted functions whose origins reflected resultant mechanisms. Contemporary homologies suggest that from non-rotary pilus, appendage secretion system, passive sensory structure. Here, we review structure, assembly, mechanism distinct solutions as foundation better understand how times highlight principles evolution.

Language: Английский

Citations

83
The structure and mechanism of the bacterial type II secretion system DOI
Souvik Naskar, Michael Höhl, Matteo Tassinari

et al.

Molecular Microbiology, Journal Year: 2020, Volume and Issue: 115(3), P. 412 - 424

Published: Dec. 7, 2020

Abstract The type II secretion system (T2SS) is a multi‐protein complex used by many bacteria to move substrates across their cell membrane. Substrates released into the environment serve as local and long‐range effectors that promote nutrient acquisition, biofilm formation, pathogenicity. In both animals plants, T2SS increasingly recognized key driver of virulence. spans bacterial envelope extrudes through an outer membrane secretin channel using pseudopilus. An inner assembly platform cytoplasmic motor controls pseudopilus assembly. This microreview focuses on structure mechanism T2SS. Advances in cryo‐electron microscopy are enabling elaborate sub‐complexes be resolved. However, questions remain regarding extension retraction, how this coupled with choreography substrate moving system. part ancient IV filament superfamily may have been present within last universal common ancestor (LUCA). Overall, mechanistic principles underlie function implication for other closely related systems such tight adherence pilus systems.

Language: Английский

Citations

77
Type IV Pili: dynamic bacterial nanomachines DOI
Courtney K. Ellison, Gregory B. Whitfield, Yves V. Brun

et al.

FEMS Microbiology Reviews, Journal Year: 2021, Volume and Issue: 46(2)

Published: Nov. 8, 2021

Bacteria and archaea rely on appendages called type IV pili (T4P) to participate in diverse behaviors including surface sensing, biofilm formation, virulence, protein secretion motility across surfaces. T4P are broadly distributed fibers that dynamically extend retract, this dynamic activity is essential for their function broad processes. Despite the essentiality of dynamics function, little known about role these molecular mechanisms controlling them. Recent advances microscopy have yielded insight into functions recent structural work has expanded what inner workings motor. This review discusses progress understanding regulation, dynamics.

Language: Английский

Citations

66