DNA nanotechnology

Nature Reviews Materials, Journal Year: 2017, Volume and Issue: 3(1)

Published: Nov. 8, 2017

Language: Английский

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A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo

Nature Biotechnology, Journal Year: 2018, Volume and Issue: 36(3), P. 258 - 264

Published: Feb. 12, 2018

Language: Английский

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DNA Origami: Scaffolds for Creating Higher Order Structures

Chemical Reviews, Journal Year: 2017, Volume and Issue: 117(20), P. 12584 - 12640

Published: June 12, 2017

DNA has become one of the most extensively used molecular building blocks for engineering self-assembling materials. origami is a technique that uses hundreds short oligonucleotides, called staple strands, to fold long single-stranded DNA, which scaffold strand, into various designer nanoscale architectures. dramatically improved complexity and scalability nanostructures. Due its high degree customization spatial addressability, provides versatile platform with engineer structures devices can sense, compute, actuate. These capabilities open up opportunities broad range applications in chemistry, biology, physics, material science, computer science have often required programmed control molecules atoms three-dimensional (3D) space. This review comprehensive survey recent developments structure, design, assembly, directed self-assembly, as well applications.

Language: Английский

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Super-resolution microscopy with DNA-PAINT

Nature Protocols, Journal Year: 2017, Volume and Issue: 12(6), P. 1198 - 1228

Published: May 18, 2017

Language: Английский

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Fluorescence nanoscopy in cell biology

Nature Reviews Molecular Cell Biology, Journal Year: 2017, Volume and Issue: 18(11), P. 685 - 701

Published: Sept. 6, 2017

Language: Английский

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Multiplex digital spatial profiling of proteins and RNA in fixed tissue

Nature Biotechnology, Journal Year: 2020, Volume and Issue: 38(5), P. 586 - 599

Published: May 1, 2020

Language: Английский

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Single-molecule localization microscopy

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: June 3, 2021

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited and can image biological structures at the molecular scale. In SMLM, individual fluorescent molecules are computationally localized from sequences localizations used to generate super-resolution or time course images, define trajectories. this Primer, we introduce basic principles SMLM before describing main experimental considerations when performing including labelling, sample preparation, hardware requirements acquisition in fixed live cells. We then explain how low-resolution processed reconstruct images and/or extract quantitative information, highlight selection discoveries enabled by closely related methods. discuss some limitations potential artefacts as well ways alleviate them. Finally, present an outlook on advanced promising new developments fast-evolving field SMLM. hope Primer will be useful reference for both newcomers practitioners

Language: Английский

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Principles and Applications of Nucleic Acid Strand Displacement Reactions

Chemical Reviews, Journal Year: 2019, Volume and Issue: 119(10), P. 6326 - 6369

Published: Feb. 4, 2019

Dynamic DNA nanotechnology, a subfield of is concerned with the study and application nucleic acid strand-displacement reactions. Strand-displacement reactions generally proceed by three-way or four-way branch migration initially were investigated for their relevance to genetic recombination. Through use toeholds, which are single-stranded segments an invader strand can bind initiate migration, rate displacement be varied more than 6 orders magnitude. In addition, toeholds enables construction enzyme-free reaction networks exhibiting complex dynamical behavior. A demonstration this was provided in year 2000, employed drive DNA-based nanomachine (Yurke, B.; et al. Nature 406, 605–608). Since then, toehold-mediated have been used ever increasing sophistication field dynamic nanotechnology has grown exponentially. Besides molecular machines, produced catalytic systems, all chemical oscillators most computers yet devised. Enzyme-free systems function as amplifiers such received considerable attention sensing detection applications chemistry medical diagnostics. combined other enzymatically driven processes also within living cells (Groves, Nat. Nanotechnol. 2015, 11, 287–294). principles applied synthetic biology enable artificial gene regulation computation bacteria. Given enormous progress over past years, now seems poised practical application.

Language: Английский

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DNA nanotechnology from the test tube to the cell

Nature Nanotechnology, Journal Year: 2015, Volume and Issue: 10(9), P. 748 - 760

Published: Sept. 1, 2015

Language: Английский

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Deep learning massively accelerates super-resolution localization microscopy

Nature Biotechnology, Journal Year: 2018, Volume and Issue: 36(5), P. 460 - 468

Published: April 16, 2018

Language: Английский

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