bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2020,
Volume and Issue:
unknown
Published: May 10, 2020
ABSTRACT
Coronavirus
disease
2019
(COVID19)
is
a
respiratory
infection
caused
by
severe
acute
syndrome
coronavirus
2
(SARS-CoV-2)
originating
in
Wuhan,
China
2019.
The
notably
elderly
and
those
with
underlying
chronic
conditions.
A
molecular
mechanism
that
explains
why
the
are
vulnerable
children
resistant
largely
unknown.
Here
we
show
loading
cells
cholesterol
from
blood
serum
using
transport
protein
apolipoprotein
E
(apoE)
enhances
entry
of
pseudotyped
SARS-CoV-2
infectivity
virion.
Super
resolution
imaging
point
high
shows
almost
twice
total
number
endocytic
points.
Cholesterol
concomitantly
traffics
angiotensinogen
converting
enzyme
(ACE2)
to
site
where
presumably
docks
efficiently
exploit
into
cell.
Furthermore,
producing
virus,
optimally
positions
furin
for
priming
SARS-CoV-2,
more
infectious
virion
improved
binding
ACE2
receptor.
In
vivo,
age
fat
diet
induces
up
40%
trafficking
sites
lung
tissue
mice.
We
propose
component
COVID19
severity
based
on
level
sensitivity
cholesterol.
Molecules
reduce
or
disrupt
localization
viral
points
producer
cells,
may
obese
patients.
Science,
Journal Year:
2018,
Volume and Issue:
361(6405), P. 880 - 887
Published: Aug. 30, 2018
Super-resolution
microscopy
has
overcome
a
long-held
resolution
barrier-the
diffraction
limit-in
light
and
enabled
visualization
of
previously
invisible
molecular
details
in
biological
systems.
Since
their
conception,
super-resolution
imaging
methods
have
continually
evolved
can
now
be
used
to
image
cellular
structures
three
dimensions,
multiple
colors,
living
systems
with
nanometer-scale
resolution.
These
been
applied
answer
questions
involving
the
organization,
interaction,
stoichiometry,
dynamics
individual
building
blocks
integration
into
functional
machineries
cells
tissues.
In
this
Review,
we
provide
an
overview
methods,
state-of-the-art
capabilities,
constantly
expanding
applications
biology,
focus
on
latter.
We
will
also
describe
current
technical
challenges
future
advances
anticipated
imaging.
Journal of the American Chemical Society,
Journal Year:
2018,
Volume and Issue:
141(7), P. 2770 - 2781
Published: Dec. 14, 2018
Super-resolution
fluorescence
microscopy
is
a
powerful
tool
to
visualize
biomolecules
and
cellular
structures
at
the
nanometer
scale.
Employing
these
techniques
in
living
cells
has
opened
up
possibility
study
dynamic
processes
with
unprecedented
spatial
temporal
resolution.
Different
physical
approaches
super-resolution
have
been
introduced
over
last
years.
A
bottleneck
apply
for
live-cell
imaging
become
availability
of
appropriate
fluorescent
probes
that
can
be
specifically
attached
biomolecules.
In
this
Perspective,
we
discuss
role
small-molecule
challenges
need
overcome
their
generation.
Recent
trends
development
labeling
strategies
are
reviewed
together
required
chemical
spectroscopic
properties
probes.
Finally,
selected
examples
use
given.
Antioxidants,
Journal Year:
2018,
Volume and Issue:
7(1), P. 13 - 13
Published: Jan. 16, 2018
Mitochondria
are
organelles
with
a
highly
dynamic
ultrastructure
maintained
by
delicate
equilibrium
between
its
fission
and
fusion
rates.
Understanding
the
factors
influencing
this
balance
is
important
as
perturbations
to
mitochondrial
dynamics
can
result
in
pathological
states.
As
terminal
site
of
nutrient
oxidation
for
cell,
powerhouses
harness
energy
form
ATP
process
driven
electron
transport
chain.
Contemporaneously,
electrons
translocated
within
chain
undergo
spontaneous
side
reactions
oxygen,
giving
rise
superoxide
variety
other
downstream
reactive
oxygen
species
(ROS).
Mitochondrially-derived
ROS
mediate
redox
signaling
or,
excess,
cause
cell
injury
even
death.
Recent
evidence
suggests
that
tightly
coupled
generation
depending
on
physiological
status
cell.
Yet,
mechanism
which
changes
shape
modulate
function
homeostasis
less
clear.
Aberrant
morphology
may
lead
enhanced
formation,
which,
turn,
deteriorate
health
further
exacerbate
oxidative
stress
self-perpetuating
vicious
cycle.
Here,
we
review
latest
findings
intricate
relationship
production,
focusing
mainly
role
malignant
disease.
Advanced Materials,
Journal Year:
2019,
Volume and Issue:
31(32)
Published: May 30, 2019
Abstract
What
to
measure
?
is
a
key
question
in
nanoscience,
and
it
not
straightforward
address
as
different
physicochemical
properties
define
nanoparticle
sample.
Most
prominent
among
these
are
size,
shape,
surface
charge,
porosity.
Today
researchers
have
an
unprecedented
variety
of
measurement
techniques
at
their
disposal
assign
precise
numerical
values
those
parameters.
However,
methods
based
on
physical
principles
probe
aspects,
only
the
particles
themselves,
but
also
preparation
history
environment
time
measurement.
Understanding
connections
can
be
great
value
for
interpreting
characterization
results
ultimately
controlling
structure–function
relationship.
Here,
current
that
enable
fundamental
presented
practical
advantages
disadvantages
discussed.
Some
recommendations
how
parameters
nanoparticles
should
investigated
fully
characterize
environments
according
intended
use
proposed.
The
intention
improve
comparability
performance
ensure
successful
transfer
scientific
knowledge
industrial
real‐world
applications.
