Nanoparticle–Biofilm Interactions: The Role of the EPS Matrix

Trends in Microbiology, Journal Year: 2019, Volume and Issue: 27(11), P. 915 - 926

Published: Aug. 13, 2019

Language: Английский

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Surface Charge Switchable Supramolecular Nanocarriers for Nitric Oxide Synergistic Photodynamic Eradication of Biofilms

ACS Nano, Journal Year: 2019, Volume and Issue: 14(1), P. 347 - 359

Published: Dec. 30, 2019

Biofilm has resulted in numerous obstinate clinical infections, posing severe threats to public health. It is urgent develop original antibacterial strategies for eradicating biofilms. Herein, we a surface charge switchable supramolecular nanocarrier exhibiting pH-responsive penetration into an acidic biofilm nitric oxide (NO) synergistic photodynamic eradication of the methicillin-resistant Staphylococcus aureus (MRSA) with negligible damage healthy tissues under laser irradiation. Originally, by integrating glutathione (GSH)-sensitive α-cyclodextrin (α-CD) conjugated prodrug (α-CD-NO) and chlorin e6 (Ce6) (α-CD-Ce6) pH-sensitive poly(ethylene glycol) (PEG) block polypeptide copolymer (PEG-(KLAKLAK)2-DA) via host–guest interaction, α-CD-Ce6-NO-DA was finely prepared. The shows complete reversal from negative at physiological pH (7.4) positive (5.5), promoting efficient biofilm. Once infiltrated biofilm, exhibits rapid NO release triggered overexpressed GSH which not only produces abundant killing bacteria but also reduces level improve therapy (PDT) efficiency. On other hand, can react reactive oxygen species (ROS) produce nitrogen (RNS), further improving PDT Due effective depletion GSH, GSH-sensitive greatly efficiency low photosensitizer dose intensity cause side effect tissues. Considering above advantages, strategy developed this work may offer great possibilities fight against infections.

Language: Английский

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Biomaterial-based antimicrobial therapies for the treatment of bacterial infections

Nature Reviews Materials, Journal Year: 2021, Volume and Issue: 7(1), P. 39 - 54

Published: Sept. 15, 2021

Language: Английский

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Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1614 - 1614

Published: June 19, 2023

Biofilm is complex and consists of bacterial colonies that reside in an exopolysaccharide matrix attaches to foreign surfaces a living organism. frequently leads nosocomial, chronic infections clinical settings. Since the bacteria biofilm have developed antibiotic resistance, using antibiotics alone treat brought on by ineffective. This review provides succinct summary theories behind composition of, formation drug-resistant attributed cutting-edge curative approaches counteract biofilm. The high frequency medical device-induced due warrants application innovative technologies manage complexities presented

Language: Английский

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Advances in Antimicrobial Microneedle Patches for Combating Infections

Advanced Materials, Journal Year: 2020, Volume and Issue: 32(33)

Published: June 29, 2020

Abstract Skin infections caused by bacteria, viruses and fungi are difficult to treat conventional topical administration because of poor drug penetration across the stratum corneum. This results in low bioavailability drugs infection site, as well lack prolonged release. Emerging antimicrobial transdermal ocular microneedle patches have become promising medical devices for delivery various antibacterial, antifungal, antiviral therapeutics. In present review, skin anatomy its barriers along with discussed. Potential strategies designing microneedles their targeted therapy outlined. Finally, biosensing associated personalized selective toxicity toward specific microbial species

Language: Английский

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Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: Oct. 29, 2020

Pathogenic microorganisms and their chronic pathogenicity are significant concerns in biomedical research. Biofilm-linked persistent infections not easy to treat due resident multidrug-resistant microbes. Low efficiency of various treatments in-vivo toxicity available antibiotics drives the researchers towards discovery many effective natural anti-biofilm agents. Natural extracts, product-based agents more efficient than chemically synthesized counterparts with lesser side effects. The present review primarily focuses on agents, i.e. phytochemicals, biosurfactants, antimicrobial peptides microbial enzymes along sources, mechanism action via interfering quorum-sensing pathways, disruption extracellular polymeric substance, adhesion inhibitory concentrations existing literature so far. This study provides a better understanding that particular molecule exhibit different mode actions biofilm activity against one pathogenic species. information can be exploited further improve therapeutic strategy by combination compounds from diverse sources.

Language: Английский

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Prevention of biofilm formation by quorum quenching

Applied Microbiology and Biotechnology, Journal Year: 2020, Volume and Issue: 104(5), P. 1871 - 1881

Published: Jan. 11, 2020

Abstract Quorum sensing (QS) is a mechanism that enables microbial communication. It based on the constant secretion of signaling molecules to environment. The main role QS regulation vital processes in cell such as virulence factor production or biofilm formation. Due still growing bacterial resistance antibiotics have been overused, it necessary search for alternative antimicrobial therapies. One them quorum quenching (QQ) disrupts QQ-driving can decrease even completely inhibit factors (including formation). There are few QQ strategies comprise use structural analogues receptor autoinductors (AI). They may be found nature designed and synthesized via chemical engineering. Many characterized enzymes with ability degrade molecules. also impede cellular cascades. different techniques used testing QS/QQ, including chromatography-mass spectroscopy, bioluminescence, chemiluminescence, fluorescence, electrochemistry, colorimetry. all enable qualitative quantitative measurements QS/QQ This article gathers information about mechanisms QQ, their effect Basic methods study well medical biotechnological applications described. Basis research described application.

Language: Английский

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A review of cinnamaldehyde and its derivatives as antibacterial agents

Fitoterapia, Journal Year: 2019, Volume and Issue: 139, P. 104405 - 104405

Published: Nov. 1, 2019

Language: Английский

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The oralome and its dysbiosis: New insights into oral microbiome-host interactions

Computational and Structural Biotechnology Journal, Journal Year: 2021, Volume and Issue: 19, P. 1335 - 1360

Published: Jan. 1, 2021

The oralome is the summary of dynamic interactions orchestrated between ecological community oral microorganisms (comprised up to approximately 1000 species bacteria, fungi, viruses, archaea and protozoa - microbiome) that live in cavity host. These form a complex ecosystem thrive environment symbiotic relationship with human However, microbial composition significantly affected by interspecies host-microbial interactions, which turn, can impact health disease status In this review, we discuss inter-species take place examine how these change from healthy (eubiotic) (dysbiotic) states. We further dysbiotic signatures associated periodontitis caries their sequalae, (e.g., tooth/bone loss pulpitis), systemic diseases diseases, such as infective endocarditis, atherosclerosis, diabetes, Alzheimer's head neck/oral cancer. then current computational techniques assess microbiome changes. Lastly, novel for modulation may help prevention treatment, including standard hygiene methods, prebiotics, probiotics, use nano-sized drug delivery systems (nano-DDS), extracellular polymeric matrix (EPM) disruption, host response modulators.

Language: Английский

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Dextran-Coated Iron Oxide Nanoparticles as Biomimetic Catalysts for Localized and pH-Activated Biofilm Disruption

ACS Nano, Journal Year: 2019, Volume and Issue: 13(5), P. 4960 - 4971

Published: Jan. 14, 2019

Biofilms are surface-attached bacterial communities embedded within an extracellular matrix that create localized and protected microenvironments. Acidogenic oral biofilms can demineralize the enamel-apatite on teeth, causing dental caries (tooth decay). Current antimicrobials have low efficacy do not target protective acidic pH biofilm. Recently, catalytic nanoparticles were shown to disrupt but lacked a stabilizing coating required for clinical applications. Here, we report dextran-coated iron oxide termed nanozymes (Dex-NZM) display strong (peroxidase-like) activity at values, with high specificity, prevent severe without impacting surrounding tissues in vivo. Nanoparticle formulations synthesized dextran coatings (molecular weights from 1.5 40 kDa used), their performance bioactivity assessed. We found 10 provided maximal activity, biofilm uptake, antibiofilm properties. Mechanistic studies indicated cores source of whereas nanoparticle surface stability blocking catalysis. Dextran-coating facilitated NZM incorporation into exopolysaccharides (EPS) structure binding biofilms, which activated hydrogen peroxide (H2O2) killing EPS-matrix breakdown. Surprisingly, enhanced selectivity toward while avoiding gingival cells. Furthermore, Dex-NZM/H2O2 treatment significantly reduced onset severity lesions (vs control or either Dex-NZM H2O2 alone) adverse effects microbiota diversity Therefore, potential as alternative tooth decay possibly other biofilm-associated diseases.

Language: Английский

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