Misguidance and modulation of axonal regeneration by Stat3 and Rho/ROCK signaling in the transparent optic nerve DOI Creative Commons
Vincent Pernet,

Sandrine Joly,

Noémie Jordi

et al.

Cell Death and Disease, Journal Year: 2013, Volume and Issue: 4(7), P. e734 - e734

Published: July 18, 2013

The use of the visual system played a major role in elucidation molecular mechanisms controlling axonal regeneration injured CNS after trauma. In this model, CNTF was shown to be most potent known neurotrophic factor for optic nerve. To clarify downstream growth regulator Stat3, we analyzed and neuronal survival an nerve crush adult mice. infection retinal ganglion cells with adeno-associated virus serotype 2 (AAV2) containing wild-type (Stat3-wt) or constitutively active (Stat3-ca) Stat3 cDNA promoted Axonal whole-mounted nerves three dimensions (3D) tissue clearing. Surprisingly, AAV2.Stat3-ca stimulation, axons elongating beyond lesion site displayed very irregular courses, including frequent U-turns, suggesting massive directionality guidance problems. pharmacological blockade ROCK, key signaling component myelin-associated inhibitors, reduced U-turns potentiated AAV2.Stat3-ca-induced regeneration. Similar results were obtained sustained delivery axotomized retina. These show important activation program regeneration, they reveal that misguidance is limiting can affect long-distance target interaction trauma CNS. correction associated improved axon

Language: Английский

Astrocyte scar formation aids central nervous system axon regeneration DOI
Mark A. Anderson, Joshua E. Burda, Yilong Ren

et al.

Nature, Journal Year: 2016, Volume and Issue: 532(7598), P. 195 - 200

Published: March 29, 2016

Language: Английский

Citations

1581
Dynamics of Oligodendrocyte Generation and Myelination in the Human Brain DOI Creative Commons
Maggie S. Y. Yeung,

Sofia Zdunek,

Olaf Bergmann

et al.

Cell, Journal Year: 2014, Volume and Issue: 159(4), P. 766 - 774

Published: Nov. 1, 2014

Language: Английский

Citations

461
Assembly of a new growth cone after axotomy: the precursor to axon regeneration DOI
Frank Bradke, James W. Fawcett, Micha E. Spira

et al.

Nature reviews. Neuroscience, Journal Year: 2012, Volume and Issue: 13(3), P. 183 - 193

Published: Feb. 15, 2012

Language: Английский

Citations

433
Nogo limits neural plasticity and recovery from injury DOI
Martin E. Schwab, Stephen M. Strittmatter

Current Opinion in Neurobiology, Journal Year: 2014, Volume and Issue: 27, P. 53 - 60

Published: March 17, 2014

Language: Английский

Citations

345
Rat models of spinal cord injury: from pathology to potential therapies DOI Creative Commons
Jacob Kjell, Lar̀s Olson

Disease Models & Mechanisms, Journal Year: 2016, Volume and Issue: 9(10), P. 1125 - 1137

Published: Oct. 1, 2016

ABSTRACT A long-standing goal of spinal cord injury research is to develop effective repair strategies for the clinic. Rat models provide an important mammalian model in which evaluate treatment and understand pathological basis injuries. These have facilitated development robust tests assessing recovery locomotor sensory functions. also allowed us how neuronal circuitry changes following could be promoted by enhancing spontaneous regenerative mechanisms counteracting intrinsic inhibitory factors. studies revealed possible routes rescuing cells acute stage injury. Spatiotemporal functional these highlight therapeutic potential manipulating inflammation, scarring myelination. In addition, replacement therapies injury, including grafts bridges, stem primarily from rat studies. Here, we discuss advantages disadvantages experimental summarize knowledge gained models. We emerging understanding different forms their pathology degree has inspired numerous strategies, some led clinical trials.

Language: Английский

Citations

343
Central Nervous System Regenerative Failure: Role of Oligodendrocytes, Astrocytes, and Microglia DOI Open Access
Jerry Silver, Martin E. Schwab, Phillip G. Popovich

et al.

Cold Spring Harbor Perspectives in Biology, Journal Year: 2014, Volume and Issue: 7(3), P. a020602 - a020602

Published: Dec. 4, 2014

Jerry Silver1, Martin E. Schwab2 and Phillip G. Popovich3 1Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio 44140 2Brain Research Institute, University Zurich Department Health Sciences Technology, ETH Zurich, 8057 Switzerland 3Center for Brain Spinal Cord Repair, State Columbus, 43210 Correspondence: phillip.popovich{at}osumc.edu

Language: Английский

Citations

310
Axonal transcription factors signal retrogradely in lesioned peripheral nerve DOI

Keren Ben‐Yaakov,

Shachar Y. Dagan,

Yael Segal-Ruder

et al.

The EMBO Journal, Journal Year: 2012, Volume and Issue: 31(6), P. 1350 - 1363

Published: Jan. 13, 2012

Language: Английский

Citations

271
Getting neural circuits into shape with semaphorins DOI
R. Jeroen Pasterkamp

Nature reviews. Neuroscience, Journal Year: 2012, Volume and Issue: 13(9), P. 605 - 618

Published: Aug. 16, 2012

Language: Английский

Citations

261
A human brain microphysiological system derived from induced pluripotent stem cells to study neurological diseases and toxicity DOI Creative Commons
David Pamies

ALTEX, Journal Year: 2016, Volume and Issue: unknown, P. 362 - 376

Published: Nov. 24, 2016

Human in vitro models of brain neurophysiology are needed to investigate molecular and cellular mechanisms associated with neurological disorders neurotoxicity. We have developed a reproducible iPSC-derived human 3D microphysiological system (BMPS), comprised differentiated mature neurons glial cells (astrocytes oligodendrocytes) that reproduce neuronal-glial interactions connectivity. BMPS over eight weeks show the critical elements neuronal function: synaptogenesis neuron-to-neuron (e.g., spontaneous electric field potentials) myelination), which mimic microenvironment central nervous system, rarely seen before. The shows 40% overall myelination after 8 differentiation. Myelin was observed by immunohistochemistry confirmed confocal microscopy reconstruction electron microscopy. These findings particular relevance since myelin is crucial for proper function development. ability assess oligodendroglial this model provide an excellent tool future studies such as multiple sclerosis other demyelinating diseases. provides suitable reliable neuron-neuroglia well pathogenic neurotoxicology.

Language: Английский

Citations

217
Progression in translational research on spinal cord injury based on microenvironment imbalance DOI Creative Commons

Baoyou Fan,

Zhijian Wei,

Shiqing Feng

et al.

Bone Research, Journal Year: 2022, Volume and Issue: 10(1)

Published: April 8, 2022

Spinal cord injury (SCI) leads to loss of motor and sensory function below the level imposes a considerable burden on patients, families, society. Repair injured spinal has been recognized as global medical challenge for many years. Significant progress made in research pathological mechanism injury. In particular, with development gene regulation, cell sequencing, tracing technologies, in-depth explorations SCI microenvironment have become more feasible. However, translational studies related repair not yielded significant results. This review summarizes latest two aspects pathology: intraneuronal imbalance regenerative imbalance. We also strategies based imbalance, including medications, transplantation, exosomes, tissue engineering, reprogramming, rehabilitation. The current state future directions are discussed. combined, precise, multitemporal strategy repairing is potential direction.

Language: Английский

Citations

138