ACS Photonics,
Journal Year:
2023,
Volume and Issue:
10(8), P. 2461 - 2466
Published: May 22, 2023
Optical
diffraction
tomography
(ODT)
is
a
powerful
tool
for
the
study
of
unlabeled
biological
cells
thanks
to
its
unique
capability
measuring
three-dimensional
(3D)
refractive
index
(RI)
distribution
samples
quantitatively
and
noninvasively.
In
conventional
transmission
ODT,
however,
certain
spatial
frequency
components
along
optical
axis
cannot
be
measured
due
limited
angular
coverage
incident
beam,
resulting
in
poor
axial
resolution
several
times
worse
than
lateral
one.
this
Letter
we
propose
new
type
ODT
method,
termed
opposite
illumination
Fourier
ptychographic
(OI-FPDT),
which
produces
almost
isotropic
by
combining
transmissive
angle-scanning
reflective
wavelength-scanning.
Without
resorting
interferometric
detection,
OI-FPDT
requires
an
intensity-only
measurement,
forward
backward
scattered
intensity
images
are
synthesized
space
recover
3D
RI
based
on
iterative
reconstruction
algorithm.
To
best
our
knowledge,
first
time
that
near-isotropic
(∼
274
nm)
result
obtained
non-interferometric
sample
motion-free
manner.
Results
simulated
cell
phantom,
tailor-made
fiberglass,
onion
epidermal
confirm
validity
proposed
method.
PhotoniX,
Journal Year:
2022,
Volume and Issue:
3(1)
Published: Oct. 21, 2022
Abstract
Quantitative
phase
imaging
(QPI)
has
emerged
as
a
valuable
tool
for
biomedical
research
thanks
to
its
unique
capabilities
quantifying
optical
thickness
variation
of
living
cells
and
tissues.
Among
many
QPI
methods,
Fourier
ptychographic
microscopy
(FPM)
allows
long-term
label-free
observation
quantitative
analysis
large
cell
populations
without
compromising
spatial
temporal
resolution.
However,
high
spatio-temporal
resolution
over
long-time
scale
(from
hours
days)
remains
critical
challenge:
optically
inhomogeneous
structure
biological
specimens
well
mechanical
perturbations
thermal
fluctuations
the
microscope
body
all
result
in
time-varying
aberration
focus
drifts,
significantly
degrading
performance
study.
Moreover,
aberrations
are
sample-
environment-dependent,
cannot
be
compensated
by
fixed
design,
thus
necessitating
rapid
dynamic
correction
process.
Here,
we
report
an
adaptive
method
based
on
annular
illumination
FPM.
In
this
method,
matched
configuration
(i.e.,
numerical
aperture
(NA)
strictly
equals
objective
NA),
which
is
key
recovering
low-frequency
information,
further
utilized
accurate
characterization.
By
using
only
6
low-resolution
images
captured
with
different
angles
matching
NA
10x,
0.4
objective,
recover
high-resolution
(synthetic
0.8)
characterize
real
time,
restoring
optimum
system
adaptively.
Applying
our
live-cell
imaging,
achieve
diffraction-limited
(full-pitch
$$655\,nm$$
655nm
at
wavelength
$$525\,nm$$
525
)
across
wide
field
view
(
$$1.77\,mm^2$$
1.772
extended
period
time.
Results in Physics,
Journal Year:
2023,
Volume and Issue:
55, P. 107183 - 107183
Published: Nov. 12, 2023
Optical
solitons
are
special
waves
that
maintain
their
shape
while
traveling.
Hence,
solitary
optical
have
great
significance
when
it
comes
to
representing
pulse
propagation
through
nonlinear
partial
differential
equations.
In
our
research,
we
uncovered
unique
solutions
for
the
formation
of
wave
patterns
in
equation
Heisenberg
ferromagnetic
spin
chain
(HFSC).
This
aptly
describes
behavior
electromagnetic
contemporary
magnetism.
By
employing
innovative
techniques
based
on
logarithmic
transformations
and
analytical
methods,
obtained
various
solution
forms
expressed
a
concise
manner
using
elementary
functions.
We
tested
correctness
these
by
substituting
them
directly
into
main
equation.
These
recent
discoveries
provide
novel
perspectives
complex
realm
as
depicted
this
model.
Moreover,
since
model
finds
applications
fiber
optic
communications,
fluid
dynamics,
other
fields,
implications
broad.
The
utilized
methods
stand
out
being
simple,
reliable,
capable
creating
fresh
equations
mathematical
physics.
research
findings
presented
here
showcase
effectiveness
applied
reliably
studying
phenomena
HFSC
problems
utilizing
tools,
scholars
opportunity
expand
enhance
understanding
frameworks
underlying
real-world
problems.
Results in Physics,
Journal Year:
2023,
Volume and Issue:
54, P. 107046 - 107046
Published: Oct. 6, 2023
This
study
focuses
on
investigating
the
optical
soliton
solutions
for
perturbed
nonlinear
Schrödinger
equation
involving
Kerr
nonlinearity.
It
should
be
noted
that
non-integrable
nature
of
becomes
apparent
when
law
nonlinearity
is
absent.
absence
integrability
significantly
obstructs
finding
exact
solutions.
Our
research
utilizes
an
innovative
analytical
technique,
modified
generalized
exponential
rational
function
method,
to
derive
new
The
we
obtained
are
quite
versatile
and
practical
real-world
applications.
They
expressed
in
terms
elementary
functions
such
as
exponentials,
trigonometric
functions,
hyperbolic
functions.
simplicity
allows
easy
comprehension
straightforward
utilization
various
scenarios.
We
present
a
variety
visualizations
graphs
enhance
understanding
physical
behaviors
exhibited
by
graphical
outcomes
give
insights
into
evolution
dynamics
under
conditions.
shows
technique's
efficacy
producing
equation.
explore
possibility
expanding
method
discover
alternative
partial
differential
equations
arise
within
theory
optics.
approach
offers
systematic
framework
obtaining
broad
class
wave
equations.
employed
methodologies
hold
also
potential
advance
field
contribute
development
tackling
these
challenging
Abstract
In
1948,
Dennis
Gabor
proposed
the
concept
of
holography,
providing
a
pioneering
solution
to
quantitative
description
optical
wavefront.
After
75
years
development,
holographic
imaging
has
become
powerful
tool
for
wavefront
measurement
and
phase
imaging.
The
emergence
this
technology
given
fresh
energy
physics,
biology,
materials
science.
Digital
holography
(DH)
possesses
advantages
wide-field,
non-contact,
precise,
dynamic
capability
complex-waves.
DH
unique
capabilities
propagation
fields
by
measuring
light
scattering
with
information.
It
offers
visualization
refractive
index
thickness
distribution
weak
absorption
samples,
which
plays
vital
role
in
pathophysiology
various
diseases
characterization
materials.
provides
possibility
bridge
gap
between
disciplines.
is
described
complex
amplitude.
complex-value
complex-domain
reconstructed
from
intensity-value
camera
real-domain.
Here,
we
regard
process
recording
reconstruction
as
transformation
real-domain,
discuss
mathematics
physical
principles
reconstruction.
We
review
underlying
principles,
technical
approaches,
breadth
applications.
conclude
emerging
challenges
opportunities
based
on
combining
other
methodologies
that
expand
scope
utility
even
further.
multidisciplinary
nature
brings
application
experts
together
label-free
cell
analytical
chemistry,
clinical
sciences,
sensing,
semiconductor
production.
Geocarto International,
Journal Year:
2024,
Volume and Issue:
39(1)
Published: Jan. 1, 2024
The
present
research
is
conducted
in
the
southern
region
of
Khyber
Pakhtunkhwa,
Pakistan,
to
identify
groundwater
potential
zones
(GWPZ).
We
used
three
models
including
Weight
Evidence
(WOE),
Frequency
Ratio
(FR),
and
Information
Value
(IV)
with
twelve
parameters
(elevation,
slope,
aspect,
curvature,
drainage
network,
LULC,
precipitation,
geology,
Lineament,
NDVI,
road,
soil
texture,
that
have
been
prepared
integrated
into
ArcGIS
10.8.
reliability
applied
models'
results
was
validated
using
Area
Under
Receiver
Operating
Characteristics
(AUROC).
GWPZ
were
reclassified
five
classes,
i.e.
very
low,
medium,
high,
high
zone.
area
occupied
by
mentioned
classes
WOE
are
low
(10.14%),
(19.58%),
medium
(26.75%),
(27.10%),
(16.40%),
while
FR
(20.93%),
(32.38%),
(18.92%),
(13.13%),
(14.61%)
IV
(14.41%),
(17.17%),
(29.01%),
(25.85%),
High
(13.53%).
Success
Rate
Curve
WOE,
FR,
0.86,
0.91,
0.87,
Predicted
values
0.89,
0.93,
0.90,
respectively.
revealed
all
statistical
performed
well
delineate
GWPZ.
However,
use
technique
strongly
encouraged
evaluate
GWPZ,
its
findings
especially
useful
for
managing
resources
urban
planning.
Our
approaches
assessing
mapping
can
be
any
similar
scenarios
recommended
as
a
helpful
tool
policymakers
manage
groundwater.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Jan. 2, 2024
Optical
tomography
has
emerged
as
a
non-invasive
imaging
method,
providing
three-dimensional
insights
into
subcellular
structures
and
thereby
enabling
deeper
understanding
of
cellular
functions,
interactions,
processes.
Conventional
optical
methods
are
constrained
by
limited
illumination
scanning
range,
leading
to
anisotropic
resolution
incomplete
structures.
To
overcome
this
problem,
we
employ
compact
multi-core
fibre-optic
cell
rotator
system
that
facilitates
precise
manipulation
cells
within
microfluidic
chip,
achieving
full-angle
projection
with
isotropic
resolution.
Moreover,
demonstrate
an
AI-driven
tomographic
reconstruction
workflow,
which
can
be
paradigm
shift
from
conventional
computational
methods,
often
demanding
manual
processing,
fully
autonomous
process.
The
performance
the
proposed
rotation
approach
is
validated
through
phantoms
HL60
human
cancer
cells.
versatility
learning-based
workflow
paves
way
for
its
broad
application
across
diverse
modalities,
including
but
not
flow
cytometry
acoustic
tomography.
Therefore,
propel
advancements
in
biology,
aiding
inception
pioneering
therapeutics,
augmenting
early-stage
diagnostics.
Optica,
Journal Year:
2022,
Volume and Issue:
9(12), P. 1362 - 1362
Published: Oct. 26, 2022
Optical
diffraction
tomography
(ODT)
is
a
promising
label-free
three-dimensional
(3D)
microscopic
method
capable
of
measuring
the
3D
refractive
index
(RI)
distribution
optically
transparent
samples
(e.g.,
unlabeled
biological
cells).
In
recent
years,
non-interferometric
ODT
techniques
have
received
increasing
attention
for
their
system
simplicity,
speckle-free
imaging
quality,
and
compatibility
with
existing
microscopes.
However,
methods
implementing
measurements
in
high
numerical
aperture
(NA)
microscopy
systems
are
often
plagued
by
low-frequency
missing
problems—a
consequence
violating
matched
illumination
condition.
Here,
we
present
transport-of-intensity
Fourier
ptychographic
(TI-FPDT)
to
address
this
challenging
issue
combining
angular
diversity
additional
“transport
intensity”
measurements.
TI-FPDT
exploits
defocused
phase
contrast
circumvent
stringent
requirement
on
NA
imposed
It
effectively
overcomes
reconstruction
quality
deterioration
RI
underestimation
problems
conventional
FPDT,
as
demonstrated
high-resolution
tomographic
various
(including
microspheres,
USAF
targets,
HeLa
cells,
C2C12
Due
its
simplicity
effectiveness,
anticipated
open
new
possibilities
biomedical
applications.