Adaptive optical imaging with entangled photons

Science, Journal Year: 2024, Volume and Issue: 383(6687), P. 1142 - 1148

Published: March 7, 2024

Adaptive optics (AO) has revolutionized imaging in fields from astronomy to microscopy by correcting optical aberrations. In label-free microscopes, however, conventional AO faces limitations because of the absence a guide star and need select an optimization metric specific sample process. Here, we propose approach leveraging correlations between entangled photons directly correct point spread function. This star–free method is independent specimen modality. We demonstrate biological samples presence aberrations using bright-field setup operating with source spatially photon pairs. Our performs better than aberrations, particularly those involving substantial defocus. work improves for could play major role development quantum microscopes.

Language: Английский

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Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Sept. 5, 2024

Language: Английский

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Neural-field-assisted transport-of-intensity phase microscopy: partially coherent quantitative phase imaging under unknown defocus distance

Photonics Research, Journal Year: 2024, Volume and Issue: 12(7), P. 1494 - 1494

Published: May 9, 2024

The transport-of-intensity equation (TIE) enables quantitative phase imaging (QPI) under partially coherent illumination by measuring the through-focus intensities combined with a linearized inverse reconstruction algorithm. However, overcoming its sensitivity to settings remains challenging problem because of difficulty in tuning optical parameters system accurately and instability long-time measurements. To address these limitations, we propose experimentally validate solution called neural-field-assisted microscopy (NFTPM) introducing tunable defocus parameter into neural field. Without weak object approximation, NFTPM incorporates physical prior image formation constrain field learns continuous representation without need for training. Simulation experimental results HeLa cells demonstrate that can achieve accurate, QPI unknown distances, providing new possibilities extending applications live cell biology.

Language: Английский

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Adaptive multiscale microscope with fast zooming, extended working distance, and large field of view

Deleted Journal, Journal Year: 2024, Volume and Issue: 5(1), P. 1 - 1

Published: Jan. 1, 2024

The field-of-view (FOV), depth of field, and resolution conventional microscopes are constrained by each other; therefore, a zoom function is required. Traditional methods lose real-time performance have limited information throughput, severely limiting their application, especially in three-dimensional dynamic imaging large-amount or large-size sample scanning. Here, an adaptive multiscale (AMS) mechanism combining the benefits liquid lenses techniques proposed to realize functions fast zooming, wide working distance (WD) range large FOV on self-developed AMS microscope. design principles were revealed. Moreover, nonuniform-distortion-correction algorithm composite patching designed improve image quality. continuous tunable magnification microscope from 9× 18×, with corresponding diameters ranging 2.31 0.98 mm 161 287 line-pairs/mm, respectively. extended WD 0.8 response time 38 ms. Experiments demonstrated advantages pathological scanning, thick-sample imaging, microfluidic process monitoring, observation living microorganisms. first step towards technology expected be applied life sciences, medical diagnosis, industrial detection.

Language: Английский

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Roadmap on computational methods in optical imaging and holography [invited]

Applied Physics B, Journal Year: 2024, Volume and Issue: 130(9)

Published: Aug. 29, 2024

Computational methods have been established as cornerstones in optical imaging and holography recent years. Every year, the dependence of on computational is increasing significantly to extent that components are being completely efficiently replaced with at low cost. This roadmap reviews current scenario four major areas namely incoherent digital holography, quantitative phase imaging, through scattering layers, super-resolution imaging. In addition registering perspectives modern-day architects above research areas, also reports some latest studies topic. codes pseudocodes presented for a plug-and-play fashion readers not only read understand but practice algorithms their data. We believe this will be valuable tool analyzing trends predict prepare future holography.

Language: Английский

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High‐Speed Multi‐Modal Extended Depth‐of‐Field Microscopy with an Electrically Tunable Lens

Laser & Photonics Review, Journal Year: 2024, Volume and Issue: 18(4)

Published: Jan. 7, 2024

Abstract High spatial resolution imaging over the entire volume for intravital of biological specimens has long been vital. However, depth‐of‐field (DOF) and are intrinsically interdependent. Here, a new extended DOF (EDOF) microscopy technique based on partially coherent annular illumination is proposed, termed AI‐EDOF, by combining an electrically tunable lens with conventional microscope modified illumination, motion‐free, high real‐time all‐in‐focus thick volumes. EDOF obtained up to near incoherent diffraction limit (388 nm, 20/ 0.8NA) temporal 30 fps. Moreover, it demonstrated that coupled phase absorption components complex refractive index in optical tomography can be solved focus scanning. The Richardson‐Lucy deconvolution total variation regularization adopted deblurring suppressing noise under low light efficiency high‐speed exposure. To demonstrate capabilities proposed method, experiments conducted using fixed transgenic zebrafish larvae, Drosophila larvae dynamic Caenorhabditis elegans both transmissive fluorescent modalities, revealing approach prospective broad applications such as pharmacokinetics tumor immunology.

Language: Английский

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Spatially‐Coded Fourier Ptychography: Flexible and Detachable Coded Thin Films for Quantitative Phase Imaging with Uniform Phase Transfer Characteristics

Advanced Optical Materials, Journal Year: 2024, Volume and Issue: 12(15)

Published: Feb. 7, 2024

Abstract Fourier ptychography (FP) is an enabling imaging technique that produces high‐resolution complex‐valued images with extended field coverages. However, when FP a phase object any specific spatial frequency, the captured contain only constant values, rendering recovery of corresponding linear ramp impossible. This challenge not unique to but also affects other common microscopy techniques — rather counterintuitive outcome given their widespread use in imaging. The underlying issue originates from non‐uniform transfer characteristic inherent microscope systems, which impedes conversion wavefields into discernible intensity variations. To address this challenge, spatially‐coded (scFP) presented for true quantitative In scFP, flexible and detachable coded thin film attached atop image sensor regular setup. modulation ensures uniform response across entire synthetic bandwidth. It improves reconstruction quality, corrects refractive index underestimation issues prevalent conventional FP, adds new dimension measurement diversity domain. development scFP expected catalyze research directions applications imaging, emphasizing need accuracy frequency response.

Language: Английский

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Microsphere-assisted super-resolution polarized light microscopy for polarization-sensitive materials

Optics Letters, Journal Year: 2025, Volume and Issue: 50(3), P. 908 - 908

Published: Jan. 2, 2025

Polarization sensitivity is a fundamental phenomenon observed in nature, and its application vital for advancing scientific discoveries. Here, we present microsphere-assisted polarized light microscopy method that enables high-quality, label-free super-resolution imaging of polarization-sensitive materials. We investigated the performance this using various samples, including colloidal crystals grating structures. Both simulations experiments were performed to explore underlying physical mechanisms driving enhancement quality. The results work have potential values fields imaging.

Language: Английский

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Adaptive Optics‐Assisted Long‐Term 3D Fluorescence and Intensity Diffraction Tomography for High Spatiotemporal Resolution Cellular Imaging

Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Abstract Intensity diffraction tomography (IDT) and fluorescence dual‐modality imaging facilitate a comprehensive analysis of biological components their interactions. However, long‐term is typically compromised by environmental thermal fluctuations mechanical disturbances from the microscope, leading to time‐varying aberrations focus drift that degrades performance accuracy colocalization. To counter these issues, an adaptive optics‐assisted 3D method (AO‐FIDT) developed. The utilizes innovative iterative ptychographic approach, paired with annular matched illumination conditions, precisely reconstruct IDT results characterize in real‐time. Furthermore, feedback on real‐time point spread function system provided synchronously correct results. efficacy precision AO‐FIDT are confirmed through long‐term, high‐resolution HeLa cells. scrutinizing morphological characteristics subcellular organelles live COS‐7 cells, including progressive sphericity mitochondria under phototoxicity, monitoring continuous changes mitochondrial dynamics throughout cell division process, broad applicability analyzing organelle structure demonstrated.

Language: Английский

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Wavelength-scanning pixel-super-resolved lens-free on-chip quantitative phase microscopy with a color image sensor

APL Photonics, Journal Year: 2024, Volume and Issue: 9(1)

Published: Jan. 1, 2024

We report a wavelength-scanning-based lens-free on-chip microscope using color CMOS sensor and matching modified phase retrieval algorithm for pixel super-resolution. Compared to traditional monochrome industrial cameras, sensors favored by the consumer electronics industry have smaller sizes, higher performance, lower costs. However, filtering array (CFA) introduces inherent modulation holograms acquired under quasi-monochromatic illumination, which complicates data processing in microscopy. Without physically removing CFA positioned on chip, we demonstrate quantitative imaging (QPI) with lateral half-width resolution of 615 nm over wide field-of-view 51.88 mm2 exploiting green-channel from Bayer-masked holograms. The resulting spatial bandwidth product is 137.2 megapixels, 10 times that conventional optical microscope. rationale only information each sampling point not lost during propagation but rather distributed all pixels image. Therefore, missing other channels can be recovered sufficient differences among raw images captured at different wavelengths. scheme sensors, this method requires acquisition several more guarantee convergence algorithm. Experimental results show achieve high-quality QPI thus demonstrating applicability cost-effective field holographic

Language: Английский

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