Biomedical Optics Express,
Journal Year:
2024,
Volume and Issue:
15(10), P. 5932 - 5932
Published: Sept. 5, 2024
Optical
diffraction
tomography
(ODT)
enables
label-free
and
morphological
3D
imaging
of
biological
samples
using
refractive-index
(RI)
contrast.
To
accomplish
this,
ODT
systems
typically
capture
multiple
angular-specific
scattering
measurements,
which
are
used
to
computationally
reconstruct
a
sample's
RI.
Standard
employ
scanning
mirrors
generate
angular
illuminations.
However,
limited
illuminating
the
sample
from
only
one
angle
at
time.
Furthermore,
when
operated
high
speeds,
these
may
exhibit
mechanical
instabilities
that
compromise
image
quality
measurement
speed.
Recently,
newer
have
been
introduced
utilize
digital-micromirror
devices
(DMD),
spatial
light
modulators
(SLMs),
or
LED
arrays
achieve
switchable
angle-scanning
with
no
physically-scanning
components.
associate
power
inefficiencies
and/or
spurious
orders
can
also
limit
performance.
In
this
work,
we
developed
novel
non-interferometric
system
utilizes
fully
module
for
composed
DMD
microlens
array
(MLA).
Compared
other
systems,
each
illumination
be
generated
independently
every
(i.e.,
orders)
while
optimizing
efficiency
based
on
required
density
angles.
We
validate
quantitative
capability
calibration
microspheres.
demonstrate
its
multiple-scattering
by
an
early-stage
zebrafish
embryo.
Photonics Research,
Journal Year:
2024,
Volume and Issue:
12(7), P. 1494 - 1494
Published: May 9, 2024
The
transport-of-intensity
equation
(TIE)
enables
quantitative
phase
imaging
(QPI)
under
partially
coherent
illumination
by
measuring
the
through-focus
intensities
combined
with
a
linearized
inverse
reconstruction
algorithm.
However,
overcoming
its
sensitivity
to
settings
remains
challenging
problem
because
of
difficulty
in
tuning
optical
parameters
system
accurately
and
instability
long-time
measurements.
To
address
these
limitations,
we
propose
experimentally
validate
solution
called
neural-field-assisted
microscopy
(NFTPM)
introducing
tunable
defocus
parameter
into
neural
field.
Without
weak
object
approximation,
NFTPM
incorporates
physical
prior
image
formation
constrain
field
learns
continuous
representation
without
need
for
training.
Simulation
experimental
results
HeLa
cells
demonstrate
that
can
achieve
accurate,
QPI
unknown
distances,
providing
new
possibilities
extending
applications
live
cell
biology.
Applied Physics B,
Journal Year:
2024,
Volume and Issue:
130(9)
Published: Aug. 29, 2024
Computational
methods
have
been
established
as
cornerstones
in
optical
imaging
and
holography
recent
years.
Every
year,
the
dependence
of
on
computational
is
increasing
significantly
to
extent
that
components
are
being
completely
efficiently
replaced
with
at
low
cost.
This
roadmap
reviews
current
scenario
four
major
areas
namely
incoherent
digital
holography,
quantitative
phase
imaging,
through
scattering
layers,
super-resolution
imaging.
In
addition
registering
perspectives
modern-day
architects
above
research
areas,
also
reports
some
latest
studies
topic.
codes
pseudocodes
presented
for
a
plug-and-play
fashion
readers
not
only
read
understand
but
practice
algorithms
their
data.
We
believe
this
will
be
valuable
tool
analyzing
trends
predict
prepare
future
holography.
Abstract
High
spatial
resolution
imaging
over
the
entire
volume
for
intravital
of
biological
specimens
has
long
been
vital.
However,
depth‐of‐field
(DOF)
and
are
intrinsically
interdependent.
Here,
a
new
extended
DOF
(EDOF)
microscopy
technique
based
on
partially
coherent
annular
illumination
is
proposed,
termed
AI‐EDOF,
by
combining
an
electrically
tunable
lens
with
conventional
microscope
modified
illumination,
motion‐free,
high
real‐time
all‐in‐focus
thick
volumes.
EDOF
obtained
up
to
near
incoherent
diffraction
limit
(388
nm,
20/
0.8NA)
temporal
30
fps.
Moreover,
it
demonstrated
that
coupled
phase
absorption
components
complex
refractive
index
in
optical
tomography
can
be
solved
focus
scanning.
The
Richardson‐Lucy
deconvolution
total
variation
regularization
adopted
deblurring
suppressing
noise
under
low
light
efficiency
high‐speed
exposure.
To
demonstrate
capabilities
proposed
method,
experiments
conducted
using
fixed
transgenic
zebrafish
larvae,
Drosophila
larvae
dynamic
Caenorhabditis
elegans
both
transmissive
fluorescent
modalities,
revealing
approach
prospective
broad
applications
such
as
pharmacokinetics
tumor
immunology.
Advanced Optical Materials,
Journal Year:
2024,
Volume and Issue:
12(15)
Published: Feb. 7, 2024
Abstract
Fourier
ptychography
(FP)
is
an
enabling
imaging
technique
that
produces
high‐resolution
complex‐valued
images
with
extended
field
coverages.
However,
when
FP
a
phase
object
any
specific
spatial
frequency,
the
captured
contain
only
constant
values,
rendering
recovery
of
corresponding
linear
ramp
impossible.
This
challenge
not
unique
to
but
also
affects
other
common
microscopy
techniques
—
rather
counterintuitive
outcome
given
their
widespread
use
in
imaging.
The
underlying
issue
originates
from
non‐uniform
transfer
characteristic
inherent
microscope
systems,
which
impedes
conversion
wavefields
into
discernible
intensity
variations.
To
address
this
challenge,
spatially‐coded
(scFP)
presented
for
true
quantitative
In
scFP,
flexible
and
detachable
coded
thin
film
attached
atop
image
sensor
regular
setup.
modulation
ensures
uniform
response
across
entire
synthetic
bandwidth.
It
improves
reconstruction
quality,
corrects
refractive
index
underestimation
issues
prevalent
conventional
FP,
adds
new
dimension
measurement
diversity
domain.
development
scFP
expected
catalyze
research
directions
applications
imaging,
emphasizing
need
accuracy
frequency
response.
Abstract
Intensity
diffraction
tomography
(IDT)
and
fluorescence
dual‐modality
imaging
facilitate
a
comprehensive
analysis
of
biological
components
their
interactions.
However,
long‐term
is
typically
compromised
by
environmental
thermal
fluctuations
mechanical
disturbances
from
the
microscope,
leading
to
time‐varying
aberrations
focus
drift
that
degrades
performance
accuracy
colocalization.
To
counter
these
issues,
an
adaptive
optics‐assisted
3D
method
(AO‐FIDT)
developed.
The
utilizes
innovative
iterative
ptychographic
approach,
paired
with
annular
matched
illumination
conditions,
precisely
reconstruct
IDT
results
characterize
in
real‐time.
Furthermore,
feedback
on
real‐time
point
spread
function
system
provided
synchronously
correct
results.
efficacy
precision
AO‐FIDT
are
confirmed
through
long‐term,
high‐resolution
HeLa
cells.
scrutinizing
morphological
characteristics
subcellular
organelles
live
COS‐7
cells,
including
progressive
sphericity
mitochondria
under
phototoxicity,
monitoring
continuous
changes
mitochondrial
dynamics
throughout
cell
division
process,
broad
applicability
analyzing
organelle
structure
demonstrated.
Cells,
Journal Year:
2024,
Volume and Issue:
13(4), P. 324 - 324
Published: Feb. 10, 2024
Fourier
ptychographic
microscopy
(FPM)
emerged
as
a
prominent
imaging
technique
in
2013,
attracting
significant
interest
due
to
its
remarkable
features
such
precise
phase
retrieval,
expansive
field
of
view
(FOV),
and
superior
resolution.
Over
the
past
decade,
FPM
has
become
an
essential
tool
microscopy,
with
applications
metrology,
scientific
research,
biomedicine,
inspection.
This
achievement
arises
from
ability
effectively
address
persistent
challenge
achieving
trade-off
between
FOV
resolution
systems.
It
wide
range
applications,
including
label-free
imaging,
drug
screening,
digital
pathology.
In
this
comprehensive
review,
we
present
concise
overview
fundamental
principles
compare
it
similar
techniques.
addition,
study
on
colorization
restored
photographs
enhancing
speed
FPM.
Subsequently,
showcase
several
utilizing
previously
described
technologies,
specific
focus
pathology,
three-dimensional
imaging.
We
thoroughly
examine
benefits
challenges
associated
integrating
deep
learning
To
summarize,
express
our
own
viewpoints
technological
progress
explore
prospective
avenues
for
future
developments.
ACS Photonics,
Journal Year:
2023,
Volume and Issue:
10(8), P. 2461 - 2466
Published: May 22, 2023
Optical
diffraction
tomography
(ODT)
is
a
powerful
tool
for
the
study
of
unlabeled
biological
cells
thanks
to
its
unique
capability
measuring
three-dimensional
(3D)
refractive
index
(RI)
distribution
samples
quantitatively
and
noninvasively.
In
conventional
transmission
ODT,
however,
certain
spatial
frequency
components
along
optical
axis
cannot
be
measured
due
limited
angular
coverage
incident
beam,
resulting
in
poor
axial
resolution
several
times
worse
than
lateral
one.
this
Letter
we
propose
new
type
ODT
method,
termed
opposite
illumination
Fourier
ptychographic
(OI-FPDT),
which
produces
almost
isotropic
by
combining
transmissive
angle-scanning
reflective
wavelength-scanning.
Without
resorting
interferometric
detection,
OI-FPDT
requires
an
intensity-only
measurement,
forward
backward
scattered
intensity
images
are
synthesized
space
recover
3D
RI
based
on
iterative
reconstruction
algorithm.
To
best
our
knowledge,
first
time
that
near-isotropic
(∼
274
nm)
result
obtained
non-interferometric
sample
motion-free
manner.
Results
simulated
cell
phantom,
tailor-made
fiberglass,
onion
epidermal
confirm
validity
proposed
method.
Advanced Photonics,
Journal Year:
2024,
Volume and Issue:
6(02)
Published: March 4, 2024
We
report
tensorial
tomographic
Fourier
ptychography
(T2oFu),
a
nonscanning
label-free
microscopy
method
for
simultaneous
imaging
of
quantitative
phase
and
anisotropic
specimen
information
in
3D.
Built
upon
ptychography,
technique,
T2oFu
additionally
highlights
the
vectorial
nature
light.
The
setup
consists
standard
microscope
equipped
with
an
LED
matrix,
polarization
generator,
polarization-sensitive
camera.
Permittivity
tensors
samples
are
computationally
recovered
from
polarized
intensity
measurements
across
three
dimensions.
demonstrate
T2oFu's
efficiency
through
volumetric
reconstructions
refractive
index,
birefringence,
orientation
various
validation
samples,
as
well
tissue
muscle
fibers
diseased
heart
tissue.
Our
healthy
reveal
their
3D
fine-filament
structures
consistent
orientations.
Additionally,
we
sample
that
carries
important
detecting
cardiac
amyloidosis.
Abstract
A
novel
high‐speed,
high‐resolution
3D
microscopy
technique
named
BP‐TIDT
is
presented
that
quantifies
the
refractive
index
(RI)
distribution
of
label‐free,
transparent
samples.
This
method
combines
a
bi‐plane
detection
scheme
(BP)
with
transport
intensity
diffraction
tomography
(TIDT),
effectively
circumventing
need
for
matched
illumination
conditions
under
high
numerical
aperture
(NA)
objectives,
which
enables
15
fps
volume
rates
and
326
nm
lateral
resolution.
The
effectiveness
accuracy
proposed
approach
are
validated
through
imaging
polystyrene
microspheres
HepG2
cells.
Moreover,
wide‐ranging
applicability
demonstrated
by
investigating
subcellular
organelle
motion,
including
mitochondria
lipid
droplets,
as
well
macroscopic
apoptosis
process
in
living
COS‐7
To
best
current
knowledge,
this
first
time
spatial‐temporal
resolution
dynamic
ODT
results
obtained
non‐interferometric
motion‐free
manner,
highlighting
potential
advancing
research
on
cellular
processes.
