Clinica Chimica Acta, Journal Year: 2023, Volume and Issue: 543, P. 117305 - 117305
Published: March 1, 2023
Language: Английский
Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)
Published: Nov. 24, 2021
Circular RNAs (circRNAs), covalently closed noncoding RNAs, are widely expressed in eukaryotes and viruses. They can function by regulating target gene expression, linear RNA transcription protein generation. The phosphoinositide 3-kinase (PI3K)/AKT signaling pathway plays key roles many biological cellular processes, such as cell proliferation, growth, invasion, migration, angiogenesis. It also a pivotal role cancer progression. Emerging data suggest that the circRNA/PI3K/AKT axis modulates expression of cancer-associated genes thus regulates tumor Aberrant regulation circRNAs is significantly associated with clinicopathological characteristics an important functions. In this review, we summarized functions PI3K-AKT-related vitro vivo assessed their associations characteristics. We further discussed diagnosis, prognostication, treatment cancers.
Language: Английский
Citations
146
Journal of Cancer, Journal Year: 2024, Volume and Issue: 15(7), P. 1916 - 1928
Published: Jan. 1, 2024
Background: Accumulating evidence indicates that non-coding RNAs (ncRNA), including long (lncRNAs) and circular (circRNAs), can function as competitive endogenous (ceRNAs) by binding to microRNAs (miRNAs) regulating host gene expression at the transcriptional or post-transcriptional level.Dysregulation in ceRNA network regulation has been implicated occurrence development of cancer.However, lncRNA/circRNA-miRNA-mRNA regulatory is still lacking nasopharyngeal carcinoma (NPC).Methods: Differentially expressed genes (DEGs) were obtained from our previous sequencing data Gene Expression Omnibus (GEO).Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes pathway (KEGG) used explore biological functions these common DEGs.Through a series bioinformatic analyses, was established.In additional, external GSE102349 test prognostic value hub mRNAs through Kaplan-Meier method.Results: We successfully constructed NPC, consisting 16 lncRNAs, 6 miRNAs, 3 circRNAs 10 found three (TOP2A, ZWINT, TTK) significantly associated with overall survival time (OS) patients. Conclusion:The revealed this study may help comprehensively elucidate mechanisms driving provide novel candidate biomarkers for evaluating prognosis NPC.
Language: Английский
Citations
18
Clinical and Translational Medicine, Journal Year: 2021, Volume and Issue: 11(7)
Published: July 1, 2021
Language: Английский
Citations
82
Journal of Experimental & Clinical Cancer Research, Journal Year: 2021, Volume and Issue: 40(1)
Published: Feb. 17, 2021
Long non-coding RNAs (lncRNAs) are widely involved in human cancers' progression by regulating tumor cells' various malignant behaviors. MAPKAPK5-AS1 has been recognized as an oncogene colorectal cancer. However, the biological role of hepatocellular carcinoma (HCC) not explored.Quantitative real-time PCR was performed to detect level HCC tissues and cell lines. The effects on growth metastasis were assessed via vitro experiments, including MTT, colony formation, EdU, flow cytometry, transwell assays, nude mice models. western blotting analysis carried out determine epithelial-mesenchymal transition (EMT) markers AKT signaling. interaction between MAPKAPK5-AS1, miR-154-5p, PLAGL2 explored luciferase reporter assay RNA immunoprecipitation. regulatory effect HIF-1α evaluated chromatin immunoprecipitation.MAPKAPK5-AS1 expression significantly elevated HCC, its overexpression associated with clinical features reduced survival. Functionally, knockdown repressed proliferation, mobility, EMT cells induced apoptosis. Ectopic contributed proliferation invasion vitro. Furthermore, silencing suppressed, while enhanced lung vivo. Mechanistically, upregulated PLAG1 like zinc finger 2 (PLAGL2) acting endogenous competing (ceRNA) sponge thereby activating EGFR/AKT Importantly, rescue experiments demonstrated that miR-154-5p/PLAGL2 axis mediated function cells. Interestingly, we found hypoxia-inducible factor 1α (HIF-1α), a transcript factor, could directly bind promoter activate transcription. regulated through form hypoxia-mediated MAPKAPK5-AS1/PLAGL2/HIF-1α signaling loop HCC.Our results reveal suggest be potential novel therapeutic target HCC.
Language: Английский
Citations
68
Biomedicine & Pharmacotherapy, Journal Year: 2021, Volume and Issue: 146, P. 112532 - 112532
Published: Dec. 11, 2021
Brain tumors are responsible for high mortality and morbidity worldwide. The brain tumor treatment depends on identification of molecular pathways involved in progression malignancy. Enhancer zeste homolog 2 (EZH2) has obtained much attention recent years field cancer therapy due to its aberrant expression capacity modulating genes by binding their promoter affecting methylation status. present review focuses EZH2 signaling including glioma, glioblastoma, astrocytoma, ependymomas, medulloblastoma rhabdoid tumors. mainly participates increasing proliferation invasion cells. However, medulloblastoma, demonstrates tumor-suppressor activity. Furthermore, can regulate response chemotherapy radiotherapy. Various function as upstream mediators lncRNAs miRNAs. Owing enzymatic activity, bind target induce affects expression. be considered an independent prognostic factor that upregulation provides undesirable prognosis. Both anti-tumor agents gene therapies such siRNA have been developed targeting therapy.
Language: Английский
Citations
45
Cancer Science, Journal Year: 2022, Volume and Issue: 113(8), P. 2681 - 2692
Published: May 31, 2022
The discovery of long noncoding RNAs (lncRNAs) has improved the understanding development and progression in various cancer subtypes. However, role lncRNAs temozolomide (TMZ) resistance glioblastoma multiforme (GBM) remains largely undefined. In this present study, differential expression was identified between U87 TMZ-resistant (TR) cells. lncRNA XLOC013218 (XLOC) drastically upregulated TR cells associated with poor prognosis glioma. Overexpression XLOC markedly increased TMZ resistance, promoted proliferation, inhibited apoptosis vitro vivo. addition, RNA-seq analysis gain-of-function or loss-of-function studies revealed that PIK3R2 potential target XLOC. Mechanistically, recruited specificity protein 1 (Sp1) transcription factor binding Sp1 to promoters PIK3R2, which elevated both mRNA levels. Finally, PIK3R2-mediated activation PI3K/AKT signaling pathway cell but apoptosis. conclusion, these data highlight vital XLOC/Sp1/PIK3R2/PI3K/AKT axis GBM resistance.
Language: Английский
Citations
33
Cancers, Journal Year: 2023, Volume and Issue: 15(13), P. 3298 - 3298
Published: June 22, 2023
Accumulating evidence supports that both long non-coding and micro RNAs (lncRNAs miRNAs) are implicated in glioma tumorigenesis progression. Poor outcome of gliomas has been linked to late-stage diagnosis mostly ineffectiveness conventional treatment due low knowledge about the early stage gliomas, which not possible observe with diagnostic approaches. The past few years witnessed a revolutionary advance biotechnology neuroscience understanding tumor-related molecules, including involved angiogenesis progression cells thus used as prognostic biomarkers well novel therapeutic targets. emerging research on lncRNAs miRNAs highlights their crucial role progression, offering new insights into disease. These hold significant potential targets, paving way for innovative approaches against glioma. This review encompasses comprehensive discussion gene regulation is responsible promotion or inhibition collects existing links between these key cancer-related molecules.
Language: Английский
Citations
19
Investigative Ophthalmology & Visual Science, Journal Year: 2023, Volume and Issue: 64(13), P. 29 - 29
Published: Oct. 18, 2023
Human corneal endothelial cells (hCECs) have been considered unable to regenerate in vivo, resulting decompensation after significant loss of hCECs. adipose-derived mesenchymal stem cell (ASC)-derived exosomes can tissues and organs. In this study, we investigated whether ASC-derived could protect CECs.We performed viability cell-cycle analyses evaluate the effect on regeneration capacity cultured Transforming growth factor-β (TGF-β) hydrogen peroxide (H2O2) were used induce biological stress CECs. The CECs was vivo. introduced into rat using electroporation, corneas injured cryoinjury. Next-generation sequencing analysis compare differentially expressed microRNAs (miRNAs) between hCEC-derived exosomes.ASC-derived induced CEC proliferation suppressed TGF-β- or H2O2-induced oxidative senescence. hCECs against endothelial-mesenchymal transition mitophagy. an vivo promoted wound healing protected endothelium cryoinjury-induced damage. revealed miRNAs for exosomes. They are involved lysine degradation, adherens junction, TGF-β signaling pathway, p53 Hippo forkhead box O (FoxO) regulation actin cytoskeleton, RNA degradation based Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis.ASC-derived by inducing a shift cycle suppressing senescence autophagy.
Language: Английский
Citations
18
Molecular Psychiatry, Journal Year: 2023, Volume and Issue: 29(4), P. 874 - 890
Published: Dec. 25, 2023
Long noncoding RNAs (lncRNAs) are sequences of 200 nucleotides or more that transcribed from a large portion the mammalian genome. While hypothesized to have variety biological roles, many lncRNAs remain largely functionally uncharacterized due unique challenges associated with their investigation. For example, some overlap other genomic loci, expressed in cell-type-specific manner, and/or differentially processed at post-transcriptional level. The CNS contains vast diversity lncRNAs, and highly abundant brain. However, interrogating lncRNA function models CNS, particularly vivo, can be complex challenging. Here we review breadth methods used investigate merits, understanding they provide respect neurodevelopment pathophysiology. We discuss remaining field recommendations assay based on current methods.
Language: Английский
Citations
16
Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(8), P. 5418 - 5440
Published: Jan. 9, 2024
Language: Английский
Citations
7