Overview and countermeasures of cancer burden in China

Science China Life Sciences, Journal Year: 2023, Volume and Issue: 66(11), P. 2515 - 2526

Published: April 13, 2023

Language: Английский

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Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: July 1, 2024

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.

Language: Английский

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Mechanisms of immune checkpoint inhibitors: insights into the regulation of circular RNAS involved in cancer hallmarks

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 4, 2024

Abstract Current treatment strategies for cancer, especially advanced are limited and unsatisfactory. One of the most substantial advances in cancer therapy, last decades, was discovery a new layer immunotherapy approach, immune checkpoint inhibitors (ICIs), which can specifically activate cells by targeting checkpoints. Immune checkpoints type immunosuppressive molecules expressed on cells, regulate degree activation avoid autoimmune responses. ICIs, such as anti-PD-1/PD-L1 drugs, has shown inspiring efficacy broad applicability across various cancers. Unfortunately, not all patients benefit remarkably from overall response rates to ICIs remain relatively low types. Moreover, primary acquired resistance pose serious challenges clinical application immunotherapy. Thus, deeper understanding molecular biological properties regulatory mechanisms is urgently needed improve options fo r current therapies. Recently, circular RNAs (circRNAs) have attracted increasing attention, only due their involvement aspects hallmarks, but also impact shaping tumor microenvironment. In this review, we systematically summarize status existing roles circRNAs Meanwhile, aim settle issue an evidence-oriented manner that involved hallmarks effects

Language: Английский

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CircPPAP2B controls metastasis of clear cell renal cell carcinoma via HNRNPC-dependent alternative splicing and targeting the miR-182-5p/CYP1B1 axis

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Jan. 6, 2024

Abstract Background Renal cell carcinoma (RCC) is one of the most common malignant tumor worldwide. Metastasis a leading case cancer-related deaths RCC. Circular RNAs (circRNAs), class noncoding RNAs, have emerged as important regulators in cancer metastasis. However, functional effects and regulatory mechanisms circRNAs on RCC metastasis remain largely unknown. Methods High-throughput RNA sequencing techniques were performed to analyze expression profiles mRNAs highly poorly invasive clear renal (ccRCC) lines. Functional experiments unveil role circPPAP2B proliferation metastatic capabilities ccRCC cells. pulldown, Mass spectrometry analysis, methylation immunoprecipitation (MeRIP), (RIP), co-immunoprecipitation (CoIP), next-generation RNA-sequencing double luciferase employed clarify molecular by which promotes Results In this study, we describe newly identified circular called circPPAP2B, overexpressed cells, determined through advanced high-throughput techniques. Furthermore, observed elevated tissues, particularly found it be associated with poor prognosis. unveiled that actively stimulates Mechanistically, interacts HNRNPC m6A-dependent manner facilitate nuclear translocation. Subcellular relocalization was dependent upon nondegradable ubiquitination stabilization an HNRNPC/Vimentin/Importin α7 ternary complex. Moreover, modulates interaction between splicing factors, PTBP1 HNPNPK, regulates pre-mRNA alternative splicing. Finally, our studies demonstrate functions miRNA sponge directly bind miR-182-5p increase CYP1B1 ccRCC. Conclusions Collectively, study provides comprehensive evidence via HNRNPC-dependent miR-182-5p/CYP1B1 axis highlights potential therapeutic target for intervention.

Language: Английский

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A Novel Conductive Polypyrrole‐Chitosan Hydrogel Containing Human Endometrial Mesenchymal Stem Cell‐Derived Exosomes Facilitated Sustained Release for Cardiac Repair

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(10)

Published: Jan. 4, 2024

Abstract Myocardial infarction (MI) results in cardiomyocyte necrosis and conductive system damage, leading to sudden cardiac death heart failure. Studies have shown that biomaterials can restore conduction, but cannot facilitate tissue regeneration. This study aims add regenerative capabilities the biomaterial by incorporating human endometrial mesenchymal stem cell (hEMSC)‐derived exosomes (hEMSC‐Exo) into poly‐pyrrole‐chitosan (PPY‐CHI), yield an injectable hydrogel effectively treat MI. In vitro, PPY‐CHI/hEMSC‐Exo, compared untreated controls, PPY‐CHI, or hEMSC‐Exo alone, alleviates H 2 O ‐induced apoptosis promotes tubule formation, while vivo, PPY‐CHI/hEMSC‐Exo improves post‐MI functioning, along with counteracting against ventricular remodeling fibrosis. All these activities are facilitated via increased epidermal growth factor (EGF)/phosphoinositide 3‐kinase (PI3K)/AKT signaling. Furthermore, properties of able resynchronize electrical transmission alleviate arrythmia. Overall, synergistically combines PPY‐CHI improve promoting angiogenesis inhibiting apoptosis, as well resynchronizing ultimately enable more effective MI treatment. Therefore, a provides dual benefits terms maintaining conductivity, facilitating long‐term exosome release sustained application their beneficial effects.

Language: Английский

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CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis

Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)

Published: June 6, 2022

Multiple lines of evidence have demonstrated that circular RNAs (circRNAs) play oncogenic or tumor-suppressive roles in various human cancers. Nevertheless, the biological functions circRNAs small cell lung cancer (SCLC) are still elusive.CircVAPA (annotated as hsa_circ_0006990) was identified by mining circRNA profiling dataset six paired SCLC tissues and RNA-seq data serum samples from 36 patients 118 healthy controls. The circVAPA expression level evaluated using quantitative real-time PCR cells tissues. Cell viability, colony formation, cycle apoptosis analysis assays vivo tumorigenesis were used to reveal circVAPA. underlying mechanism investigated Western blot, RNA pulldown, immunoprecipitation, dual-luciferase reporter assay rescue experiments.We revealed circVAPA, derived exons 2-4 vesicle-associated membrane protein-associated protein A (VAPA) gene, exhibited higher levels lines, clinical tissues, than controls, facilitated progression vitro vivo. Mechanistically, activated phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway modulating miR-377-3p miR-494-3p/insulin-like growth factor 1 receptor (IGF1R) axis accelerate progression. Furthermore, depletion markedly enhanced inhibitory effects BMS-536924, an IGF1R inhibitor cellular xenograft mouse models.CircVAPA promotes via miR-494-3p/IGF1R/AKT axis. We hope develop protocols combinations inhibition BMS-536924 addition for treating with upregulation.

Language: Английский

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Circular RNA circPOLR2A promotes clear cell renal cell carcinoma progression by facilitating the UBE3C-induced ubiquitination of PEBP1 and, thereby, activating the ERK signaling pathway

Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)

Published: July 15, 2022

Increasing evidence has demonstrated that circular RNAs (circRNAs) are implicated in cancer progression. However, the aberrant expression and biological functions of circRNAs clear cell renal carcinoma (cRCC) remain largely elusive.Differentially expressed cRCC were filtered via bioinformatics analysis. Aberrant circPOLR2A was validated tissues lines qRT-PCR. Sanger sequencing used to identify backsplicing site circPOLR2A. In vitro vivo functional experiments performed evaluate role malignancy. RNA pull-down, mass spectrometry, RIP, FISH immunofluorescence assays validate circPOLR2A-interacting proteins. Ubiquitination modification interaction between proteins detected Co-IP western blotting. The m6A by meRIP assay.Bioinformatics analysis revealed highly metastatic tissues. CircPOLR2A associated with tumor size TNM stage patients. accelerated proliferation, migration, invasion angiogenesis, while inhibiting apoptosis. Further mechanistic research suggested could interact UBE3C PEBP1 proteins, act as a specific ubiquitin E3 ligase for protein. UBE3C/circPOLR2A/PEBP1 protein-RNA ternary complex enhanced UBE3C-mediated ubiquitination degradation protein which inactivate ERK signaling pathway. Rescue downstream target Furthermore, confirmed, reader YTHDF2 regulate expression.Our study modulated protein, further activated pathway during progression metastasis. reader, YTHDF2, regulated cRCC. Hence, be potential diagnosis treatment

Language: Английский

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Targeting PI3K/Akt signaling in prostate cancer therapy

Journal of Cell Communication and Signaling, Journal Year: 2022, Volume and Issue: 17(3), P. 423 - 443

Published: Nov. 11, 2022

Language: Английский

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Extensive review on breast cancer its etiology, progression, prognostic markers, and treatment

Medical Oncology, Journal Year: 2023, Volume and Issue: 40(8)

Published: July 14, 2023

Language: Английский

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CircHERC1 promotes non-small cell lung cancer cell progression by sequestering FOXO1 in the cytoplasm and regulating the miR-142-3p-HMGB1 axis

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Nov. 6, 2023

Noncoding RNAs such as circular (circRNAs) are abundant in the human body and influence occurrence development of various diseases. Non-small cell lung cancer (NSCLC) is one most common malignant cancers. Information on functions mechanism circRNAs limited; thus, topic needs more exploration. The purpose this study was to identify aberrantly expressed cancer, unravel their roles NSCLC progression, provide new targets for diagnosis therapy.High-throughput sequencing used analyze differential circRNA expression patients with cancer. qRT‒PCR determine level circHERC1 tissues plasma samples. Gain- loss-of-function experiments were implemented observe impacts growth, invasion, metastasis cells vitro vivo. Mechanistically, dual luciferase reporter assays, fluorescence situ hybridization (FISH), RNA immunoprecipitation (RIP) pull-down performed confirm underlying mechanisms circHERC1. Nucleocytoplasmic localization FOXO1 determined by nucleocytoplasmic isolation immunofluorescence. interaction verified pull-down, western blot assays. proliferation migration vivo subcutaneous tail vein injection nude mice.CircHERC1 significantly upregulated cells, ectopic strikingly facilitated proliferation, invasion metastasis, inhibited apoptosis However, knockdown exerted opposite effects. CircHERC1 mainly distributed cytoplasm. Further mechanistic research indicated that acted a competing endogenous miR-142-3p relieve repressive effect its target HMGB1, activating MAPK/ERK NF-κB pathways promoting invasion. More importantly, we found could bind sequester it cytoplasm, adjusting feedback AKT pathway. accumulation cytosol nuclear exclusion promoted apoptosis. promotes tumor function may serve potential prognostic biomarker therapeutic NSCLC.CircHERC1 NSCLC. Our findings indicate facilitates regulating miR-142-3p/HMGB1 axis pathways. In addition, can promote inhibit sequestering cytoplasm regulate activity BIM transcription.

Language: Английский

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