Experimental temperatures shape host microbiome diversity and composition

Global Change Biology, Journal Year: 2022, Volume and Issue: 29(1), P. 41 - 56

Published: Oct. 17, 2022

Global climate change has led to more extreme thermal events. Plants and animals harbour diverse microbial communities, which may be vital for their physiological performance help them survive stressful climatic conditions. The extent microbiome communities in response warming or cooling important predicting host under global change. Using a meta-analysis of 1377 microbiomes from 43 terrestrial aquatic species, we found decrease the amplicon sequence variant-level phylogenetic diversity alteration composition both experimental cooling. Microbiome beta dispersion was not affected by temperature changes. We showed that habitat factors than biological traits. In particular, organisms-especially marine habitats-experienced greater depletion cold conditions, compared hosts. Exposure involving sudden long static shift associated with loss, but this reduction attenuated prior-experimental lab acclimation when ramped regime (i.e., warming) used. Microbial differential abundance co-occurrence network analyses revealed several potential indicator bacterial classes hosts heated environments on different biome levels. Overall, our findings improve understanding impact changes animal plant structures across range habitats. next step is link these measures fitness, as well community functions, determine whether can buffer some species against thermally variable world.

Language: Английский

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Gut microbiota modulation enhances the immune capacity of lizards under climate warming

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Feb. 22, 2024

Abstract Background Host-microbial interactions are expected to affect species’ adaptability climate change but have rarely been explored in ectothermic animals. Some studies shown that short-term warming reduced gut microbial diversity could hamper host functional performance. Results However, our longitudinal experiments semi-natural conditions demonstrated decreased microbiota at 2 months, increased 13 and 27 months a desert lizard ( Eremias multiocellata ). Simultaneously, long-term significantly the antibacterial activity of serum, immune responses (higher expression intestinal immune-related genes), concentration short-chain fatty acids (thereby barrier immunity) lizard. Fecal transplant further revealed enhanced response lizards. More specifically, immunity is likely due higher relative abundance Bacteroides lizards, given bacteria fragilis regulated IFN-β increase lizards under climate. Conclusions Our study suggests can help ectotherms cope with by enhancing response, highlights importance on host-microbial their biological impacts. Graphical

Language: Английский

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Microbiome Stability Is Linked to Acropora Coral Thermotolerance in Northwestern Philippines

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(2)

Published: Jan. 31, 2025

Corals associate with a diverse community of prokaryotic symbionts that provide nutrition, antioxidants and other protective compounds to their host. However, the influence microbes on coral thermotolerance remains understudied. Here, we examined microbial communities associated colonies Acropora cf. tenuis exhibit high or low upon exposure 33°C (heated) relative 29°C (control). Using 16S rRNA sequencing, show structure all A. was similar each at control temperature. Thermotolerant colonies, however, had relatively greater abundance Endozoicomonas, Arcobacter, Bifidobacterium Lactobacillus. At elevated temperature, only thermosensitive showed distinct shift in microbiome, an increase Flavobacteriales, Rhodobacteraceae Vibrio, accompanying marked bleaching response. Functional prediction indicated thermotolerant corals were enriched for genes related metabolism, while microbiomes cell motility antibiotic compound synthesis. These differences may contribute variable performance under thermal stress. Identification taxa correlated provides insights into beneficial bacterial groups could be used microbiome engineering support reef health changing climate.

Language: Английский

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Symbiosis and host responses to heating

Trends in Ecology & Evolution, Journal Year: 2022, Volume and Issue: 37(7), P. 611 - 624

Published: April 29, 2022

Language: Английский

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How does heat stress affect sponge microbiomes? Structure and resilience of microbial communities of marine sponges from different habitats

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 9

Published: Jan. 19, 2023

Introduction Sponges are key components of marine benthic communities, providing many ecosystem functions and establishing close relationships with microorganisms, conforming the holobiont. These symbiotic microbiotas seem to be host species-specific highly diverse, playing roles in their sponge host. The effects elevated seawater temperature on sponges microbiota still poorly known, whether from polar areas more sensitive these impacts respect temperate tropical species is totally unknown. Methods We analyzed microbiomes different natural habitat after exposure heat stress aquaria by 16S rRNA amplicon sequencing (1) characterize covering a latitudinal gradient (polar, environments), (2) asses thermal microbial communities. Results Bacterial communities’ structure was also surrounding seawater. core microbiome maintained most stress, although they would recover normal conditions previous remains yet further investigated. observed increased abundances transient bacteria unknown origin exposed stress. Discussion Some may opportunistic that benefit stress-associated dysregulation occupying new niches According our results, Antarctic waters could resilient than sponges. Both composition changes produced quite species-specific, thus, depend species. Under global change scenario, will probably those suffering therefore dramatic for ecosystems since fundamental part them.

Language: Английский

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Antibiotics reduce Pocillopora coral‐associated bacteria diversity, decrease holobiont oxygen consumption and activate immune gene expression

Molecular Ecology, Journal Year: 2023, Volume and Issue: 32(16), P. 4677 - 4694

Published: June 15, 2023

Abstract Corals are important models for understanding invertebrate host–microbe interactions; however, to fully discern mechanisms involved in these relationships, experimental approaches manipulating coral–bacteria associations needed. Coral‐associated bacteria affect holobiont health via nutrient cycling, metabolic exchanges and pathogen exclusion, yet it is not understood how bacterial community shifts physiology. In this study, a combination of antibiotics (ampicillin, streptomycin ciprofloxacin) was used disrupt the communities 14 colonies reef framework‐building corals Pocillopora meandrina P . verrucosa, originally collected from Panama hosting diverse algal symbionts (family Symbiodiniaceae ). photochemical efficiencies oxygen consumption (as proxies coral health) were measured throughout 5‐day exposure. Antibiotics altered composition reduced alpha beta diversity, several persisted, leading hypothesis that either resistant or occupy internal niches shielded antibiotics. While did efficiency, antibiotics‐treated had lower rates. RNAseq revealed increased expression immunity stress response genes at expense cellular maintenance metabolism functions. Together, results reveal antibiotic disruption corals' native negatively impacts by decreasing activating host without directly impairing photosynthesis, underscoring critical role coral‐associated health. They also provide baseline future experiments manipulate symbioses first reducing diversity complexity bacteria.

Language: Английский

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Global patterns in symbiont selection and transmission strategies in sponges

Frontiers in Ecology and Evolution, Journal Year: 2022, Volume and Issue: 10

Published: Oct. 26, 2022

Sponges host dense and diverse communities of microbes (known as the microbiome) beneficial for nutrition defense. Symbionts in turn receive shelter metabolites from sponge host, making their relationship both partners. Given that sponge-microbes associations are fundamental survival both, especially sponge, such is maintained through life even passed on to future generations. In many organisms, microbiome has profound effects development but influence reproductive developmental pathways sponges less understood. sponges, oocytes, sperm, embryos, larvae vertical transmission), using a variety methods include direct uptake mesohyl phagocytosis by oocytes indirect transmission oocyte nurse cells. Such can remain elements untouched, transfer offspring, or be digested make yolky nutrient reserves larvae. When how those decisions made fundamentally unanswered questions reproduction. Here we review diversity modes existent entire phylum Porifera detailed imaging electron microscopy, available metabarcoding data elements, macroevolutionary patterns associated phylogenetic constraints. Additionally, examine fidelity this possible reasons observed variability some stages. Our current understanding marine however, adult microbial community established combination horizontal (acquisition surrounding environment each new generation) processes, although extent which mode shapes still remains determined. We also assessed role filtration, cellular structures acquiring external microbes, immune system, ultimately stable prokaryotes sponges.

Language: Английский

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Shark Microbiome Analysis Demonstrates Unique Microbial Communities in Two Distinct Mediterranean Sea Shark Species

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 557 - 557

Published: March 11, 2024

Our knowledge regarding the role of microbiome in fish health has been steadily increasing last decade, especially for species commercial interest. Conversely, relatively few studies focus on microbiomes wild fish, apex predators like sharks, due to lower economic interest and greater difficulty obtaining samples. Studies investigating differences between diverse anatomical locations sharks are limited, majority available focused microbial diversity present shark teeth, with aim preventing infections bites these animals or evaluating presence certain pathogens healthy diseased specimens. Here, we investigated skin, mouth, gills, cloaca five individuals two phylogenetically distant (Prionace glauca Somniosus rostratus) obtain a better understanding animals, how they change throughout different body parts, much influenced determined by ecology evolutionary relationship host microbiome. To confirm taxonomy under study, barcoded specimens sequencing mtDNA COI from biopsy their skin. Microbial based 16S rRNA gene reveals that partially overlapping inhabit parts each species, while communities distinct species. results suggest sharks’ species-specific controlled This is first study comparatively analyzing Mediterranean Sea.

Language: Английский

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Elevated temperature promotes methane-dependent arsenate reduction in paddy soils

Soil Biology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109800 - 109800

Published: April 1, 2025

Language: Английский

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The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Sponge-associated microbes play fundamental roles in regulating their hosts' physiology, yet contribution to sexual reproduction has been largely overlooked. Most studies have concentrated on the proportion of microbiome transmitted from parents offspring, providing little evidence putative microbial role during gametogenesis sponges. Here, we use 16S rRNA gene analysis assess whether composition five gonochoristic sponge species differs between reproductive and non-reproductive individuals correlate these changes with gametogenic stages. In sponges mature oocytes, status did not influence either beta or alpha diversity. However, two studied species, Geodia macandrewii Petrosia ficiformis, which presented oocytes at previtellogenic stage, significant were detected individuals. These disparities primarily driven by differentially abundant taxa affiliated Nitrososphaeria archaeal class both species. We speculate that stages are more energetically demanding, leading due phagocytosis meet nutritional demands this period. Supporting our hypothesis, observed transcriptomic differences G. macandrewii, mainly associated immune system, indicating potential sponge's recognition system. Overall, provide new insights into possible microbiomes periods, potentially uncovering critical interactions support success. Our research explores fascinating relationship resident microbes, focusing specifically how might reproduction. Sponges marine animals known for complex beneficial partnerships various microbes. While previous looked passed parent study is among first examine communities change different By analyzing discovered occur premature suggesting may a crucial necessary nutrients early egg development. This work only enhances understanding biology but also opens up avenues studying success hosts environments.

Language: Английский

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