Molecular Therapy — Nucleic Acids,
Journal Year:
2020,
Volume and Issue:
21, P. 792 - 803
Published: July 15, 2020
Emerging
knowledge
indicates
that
non-coding
RNAs,
including
microRNAs
(miRNAs)
and
long-noncoding
RNAs
(lncRNAs),
have
a
pivotal
role
in
bone
development
the
pathogenesis
of
bone-related
disorders.
Most
recently,
miRNAs
started
to
be
regarded
as
potential
biomarkers
or
targets
for
various
sets
diseases,
while
lncRNAs
gained
attention
new
layer
gene
expression
control
acting
through
versatile
interactions,
also
with
miRNAs.
The
rapid
RNA
sequencing
techniques
based
on
next-generation
(NGS)
gives
us
better
insight
into
molecular
pathways
regulated
by
miRNA-lncRNA
network.
In
this
review,
we
summarize
current
related
function
regulators
genes
are
crucial
proper
metabolism
homeostasis.
We
characterized
important
their
signatures,
relationship
bone.
Analysis
biological
lncRNAs,
well
network,
will
pave
way
understanding
think
may
lead
innovative
diagnostic
tools
therapeutic
approaches
International Journal of Dairy Technology,
Journal Year:
2020,
Volume and Issue:
73(4), P. 706 - 717
Published: March 3, 2020
The
current
study
aimed
to
investigate
the
anti‐osteoporotic
effects
of
a
Lactobacillus
plantarum
B719‐fermented
milk
product
(FMP‐B719).
Higher
proliferation
and
mineralisation
were
observed
in
FMP‐B719‐treated
MC3T3‐E1
mouse
pre‐osteoblastic
cells
than
those
L.
casei
ATCC
393‐FMP‐treated
cells.
Moreover,
FMP‐B719
normalised
serum
alkaline
phosphatase
(9.37
±
1.92
U/L)
phosphorus
(8.28
0.1
mg/dL)
rat
model
ovariectomy‐induced
postmenopausal
osteoporosis.
Furthermore,
normalized
bone
volume
proximal
femur
increased
strength
that
was
decreased
ovariectomized
rats.
Hence,
results
this
suggest
could
serve
as
potential
candidate
for
treatment
prevention
International Journal of Molecular Sciences,
Journal Year:
2019,
Volume and Issue:
20(24), P. 6229 - 6229
Published: Dec. 10, 2019
Osteoporosis,
a
disease
characterized
by
both
loss
of
bone
mass
and
structural
deterioration
bone,
is
the
most
common
reason
for
broken
among
elderly.
It
known
that
attenuated
differentiation
ability
osteogenic
cells
has
been
regarded
as
one
greatest
contributors
to
age-related
formation
reduction.
However,
effects
current
therapies
are
still
unsatisfactory.
In
this
study
we
identify
novel
long
noncoding
RNA
AK045490
which
correlated
with
enriched
in
skeletal
tissues
mice.
vitro
analysis
bone-derived
mesenchymal
stem
(BMSCs)
showed
inhibited
osteoblast
differentiation.
vivo
inhibition
its
small
interfering
rescued
ovariectomized
osteoporosis
mice
model.
Mechanistically,
nuclear
translocation
β-catenin
downregulated
expression
TCF1,
LEF1,
Runx2.
The
results
suggest
Lnc-AK045490
suppresses
β-catenin/TCF1/Runx2
signaling
inhibits
formation,
providing
mechanism
potential
drug
target
osteoporosis.
Stem Cell Research & Therapy,
Journal Year:
2022,
Volume and Issue:
13(1)
Published: Sept. 29, 2022
Dental
follicles
are
necessary
for
tooth
eruption,
surround
the
enamel
organ
and
dental
papilla,
regulate
both
formation
resorption
of
alveolar
bone.
follicle
progenitor
cells
(DFPCs),
which
stem
found
in
follicles,
differentiate
into
different
kinds
that
eruption.
Runt-related
transcription
factor
2
(Runx2)
is
a
essential
osteoblasts
osteoclasts
differentiation,
as
well
bone
remodeling.
Mutation
Runx2
causing
cleidocranial
dysplasia
negatively
affects
osteogenesis
osteoclastic
ability
resulting
eruption
difficulties.
Among
variety
molecules,
Nel-like
molecule
type
1
(Nell-1)
plays
an
important
role
neural
crest-derived
tissues
strongly
expressed
follicles.
Nell-1
was
originally
identified
pathologically
fused
fusing
sutures
patients
with
unilateral
coronal
synostosis,
it
indispensable
roles
remodeling,
including
osteoblast
regeneration,
craniofacial
skeleton
development,
differentiation
many
cells.
proven
to
directly
target
gene
its
expression.
These
studies
suggested
Runx2/Nell-1
axis
may
play
process
by
affecting
DFPCs.
Studies
on
short
long
regulatory
noncoding
RNAs
have
revealed
complexity
RNA-mediated
regulation
expression
at
posttranscriptional
level.
This
ceRNA
network
participates
complex
way.
However,
non-study
indicated
potential
connection
between
Nell-1,
further
researches
still
needed.
Frontiers in Cell and Developmental Biology,
Journal Year:
2022,
Volume and Issue:
10
Published: Dec. 23, 2022
Retinoblastoma
(RB)
is
a
rare
aggressive
intraocular
malignancy
of
childhood
that
has
the
potential
to
affect
vision,
and
can
even
be
fatal
in
some
children.
While
tumor
controlled
efficiently
at
early
stages,
metastatic
tumors
lead
high
mortality.
Non-coding
RNAs
(ncRNAs)
are
implicated
number
physiological
cellular
process,
including
differentiation,
proliferation,
migration,
invasion,
The
deregulation
ncRNAs
correlated
with
several
diseases,
particularly
cancer.
categorized
into
two
main
groups
based
on
their
length,
i.e.
short
long
ncRNAs.
Moreover,
ncRNA
been
demonstrated
play
role
pathogenesis
development
RB.
Several
ncRNAs,
such
as
miR-491-3p,
miR-613,and
SUSD2
have
found
act
suppressor
genes
RB,
but
other
circ-E2F3,
NEAT1,
TUG1
promoter
genes.
Understanding
regulatory
mechanisms
provide
new
opportunities
for
RB
therapy.
In
present
review,
we
discuss
functional
roles
most
important
interaction
responsible
initiation
progression,
possible
future
clinical
applications
diagnostic
prognostic
tools
or
therapeutic
targets.
Current Osteoporosis Reports,
Journal Year:
2023,
Volume and Issue:
21(3), P. 289 - 302
Published: April 29, 2023
Circular
RNAs
(circRNAs)
are
RNA
transcripts
derived
from
fragments
of
pre-messenger
through
a
back-splicing
process.
An
advantage
that
rises
their
circular
covalently
closed
conformation
is
high
stability,
when
compared
with
linear
counterparts.
The
current
review
focuses
on
the
emerging
roles
circRNAs
in
osteoporosis,
including
osteogenic
differentiation
and
osteoclastogenesis.
Their
potential
as
osteoporosis
biomarkers
will
also
be
discussed.
Journal of Molecular Endocrinology,
Journal Year:
2018,
Volume and Issue:
61(4), P. 231 - 239
Published: Oct. 11, 2018
Human
corneal
fibroblasts
(HCFs)
are
implicated
in
neovascularization
(CRNV).
The
mechanisms
underlying
the
inflammatory
response
HCFs
and
development
of
CRNV
were
explored
this
study.
Alkali
burns
applied
to
corneas
rats
establish
a
model.
expression
long
noncoding
RNA
(lncRNA)
nuclear
enriched
abundant
transcript
1
(NEAT1)
mRNA
protein
levels
factor
kappa
B
(NF-κB)-
activating
(NKAP)
examined
by
quantitative
real-time
(qRT-PCR)
Western
blot
methods,
respectively.
Lipopolysaccharide
(LPS)
is
used
stimulate
for
response.
level
inflammation
factors
HCF
supernatant
was
detected
using
an
enzyme-linked
immunosorbent
assay
(ELISA).
Binding
interactions
between
NEAT1
miRNA
1246
(miR-1246)
determined
immunoprecipitation
(RIP)
pull-down
assays
HCFs.
Compared
with
control
group
(
n
=
6),
upregulated
rat
model
6).
LPS.
Downregulation
suppressed
secretion
tumor
necrosis
factor-α
(TNF-α)
interleukin-6
(IL-6).
could
bind
interact
miR-1246.
LPS
regulated
NKAP
NF-κB
signaling
via
NEAT1/miR-1246
pathway.
vivo
inhibited
progression
lncRNA
induced
factors,
mediated
NF-κB,
targeting
miR-1246,
thereby
promoting
progression.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(6), P. 5965 - 5965
Published: March 22, 2023
Osteoporosis,
a
common
systematic
bone
homeostasis
disorder
related
disease,
still
urgently
needs
innovative
treatment
methods.
Several
natural
small
molecules
were
found
to
be
effective
therapeutics
in
osteoporosis.
In
the
present
study,
quercetin
was
screened
out
from
library
of
molecular
compounds
by
dual
luciferase
reporter
system.
Quercetin
upregulate
Wnt/β-catenin
while
inhibiting
NF-κB
signaling
activities,
and
thereby
rescuing
osteoporosis-induced
tumor
necrosis
factor
alpha
(TNFα)
impaired
BMSCs
osteogenesis.
Furthermore,
putative
functional
lncRNA,
Malat1,
shown
key
mediator
regulated
activities
TNFα-impaired
osteogenesis,
as
mentioned
above.
an
ovariectomy
(OVX)-induced
osteoporosis
mouse
model,
administration
could
significantly
rescue
OVX-induced
loss
structure
deterioration.
Serum
levels
Malat1
also
obviously
rescued
OVX
model
after
treatment.
conclusion,
our
study
demonstrated
that
osteogenesis
vitro
vivo,
Malat1-dependent
manner,
suggesting
may
serve
therapeutic
candidate
for
Frontiers in Nanotechnology,
Journal Year:
2025,
Volume and Issue:
7
Published: March 19, 2025
Ribonucleic
acid-lipid
nanoparticle
(RNA-LNP)
therapeutics,
a
powerful
nanomedicine
platform,
have
already
demonstrated
their
efficacy
in
diverse
applications.
Their
improved
stability
and
are
exemplified
by
successful
rapid
launch
of
mRNA
vaccines,
as
well
marketed
siRNA
drug
product.
Beyond
infectious
diseases,
RNA-LNPs
show
promise
addressing
unmet
needs
women’s
health,
for
instance,
gynecologic
cancers
(e.g.,
ovarian,
cervical)
novel
treatments
conditions
such
osteoporosis,
endometriosis,
congenital
disorders.
However,
important
challenges
persist,
including
off-target
effects,
immunogenicity,
potential
risks
ethical
issues
application
pregnant
or
lactating
women.
This
review
summarizes
current
key
preclinical
clinical
progress,
discusses
targeting
strategies
LNPs
active
passive
delivery),
presents
knowledge
on
RNA-LNP
safety
non-pregnant
women
neonates
vulnerable
populations.
As
technologies
evolve
–
with
relevant
animal
models,
next-generation
RNA
platforms
lipid
chemistries
they
can
hold
significant
transforming
care
health
through
safer,
effective,
personalized,
innovative
curative
interventions.
