Plant SWEET Family of Sugar Transporters: Structure, Evolution and Biological Functions

Biomolecules, Journal Year: 2022, Volume and Issue: 12(2), P. 205 - 205

Published: Jan. 25, 2022

The SWEET (sugars will eventually be exported transporter) family was identified as a new class of sugar transporters that function bidirectional uniporters/facilitators and facilitate the diffusion sugars across cell membranes along concentration gradient. SWEETs are found widely in plants play central roles many biochemical processes, including phloem loading for long-distance transport, pollen nutrition, nectar secretion, seed filling, fruit development, plant-pathogen interactions responses to abiotic stress. This review focuses on advances plant SWEETs, details about their discovery, characteristics protein structure, evolution physiological functions. In addition, we discuss applications breeding. provides more in-depth comprehensive information help elucidate molecular basis plants.

Language: Английский

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Key transcription factors regulate fruit ripening and metabolite accumulation in tomato

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(3), P. 2256 - 2273

Published: April 2, 2024

Abstract Fruit ripening is a complex process involving dynamic changes to metabolites and controlled by multiple factors, including transcription factors (TFs). Several TFs are reportedly essential regulators of tomato (Solanum lycopersicum) fruit ripening. To evaluate the effects specific on metabolite accumulation during ripening, we combined CRISPR/Cas9-mediated mutagenesis with metabolome transcriptome analyses explore regulatory mechanisms. Specifically, generated various genetically engineered lines that differed regarding contents colors. The transcript profiles indicated selected have distinct functions control contents, especially carotenoids sugars. Moreover, mutation ELONGATED HYPOCOTYL5 (HY5) increased fructose glucose approximately 20% (relative wild-type levels). Our in vitro assay showed HY5 can bind directly G-box cis-element Sugars Will Eventually be Exported Transporter (SWEET12c) promoter activate expression, thereby modulating sugar transport. findings provide insights into mechanisms regulating metabolic networks, providing theoretical basis for breeding horticultural crops produce diverse flavors

Language: Английский

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The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 192(3), P. 1858 - 1876

Published: March 13, 2023

Abstract Sugars are fundamental to plant developmental processes. For fruits, the accumulation and proportion of sugars play crucial roles in development quality attractiveness. In citrus (Citrus reticulata Blanco.), we found that difference sweetness between mature fruits “Gongchuan” its bud sport “Youliang” is related hexose contents. Expression a SuS (sucrose synthase) gene CitSUS5 SWEET (sugars will eventually be exported transporter) CitSWEET6, characterized by transcriptome analysis at different stages these 2 varieties, revealed higher expression levels fruit. The CitSWEET6 were investigated enzyme activity transient assays. promoted cleavage sucrose hexoses, was identified as fructose transporter. Further investigation transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) contributes metabolism transportation positively regulating CitSWEET6. role fruit sugar homologous overexpression, -VIGS, -RNAi. modulates mediating expression, molecular mechanism explained differences composition

Language: Английский

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Abscisic acid and regulation of the sugar transporter gene MdSWEET9b promote apple sugar accumulation

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 192(3), P. 2081 - 2101

Published: Feb. 23, 2023

Abstract Enhancing fruit sugar contents, especially for high-flavonoid apples with a sour taste, is one of the main goals horticultural crop breeders. This study analyzed accumulation and underlying mechanisms in F2 progenies hybridization between high-sugar apple (Malus × domestica) variety “Gala” germplasm “CSR6R6”. We revealed that MdSWEET9b (sugars will eventually be exported transporter) helps mediate fruits. Functional characterization yeast mutants lacking transport as well overexpressing CRISPR/Cas9 knockdown calli could sucrose specifically, ultimately promoting normal growth total contents. Moreover, MdWRKY9 bound to promoter regulated its activity, which responded abscisic acid (ABA) signaling. Furthermore, interacted MdbZIP23 (basic leucine zipper) MdbZIP46, key ABA signal transducers, at protein DNA levels enhance regulatory effect on expression, thereby influencing accumulation. Based contents lines differing effects treatments fruits calli, we factors responsible diversity content. The results this have clarified how influences accumulation, while also further elucidating ABA-signaling network work provides basis future explorations crosstalk hormone metabolism pathways.

Language: Английский

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The genomic route to tomato breeding: Past, present, and future

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(4), P. 2500 - 2514

Published: April 30, 2024

Over the past 10,000 years, tomato species have undergone both unintentional and intentional selection to enhance their favorable traits for human consumption manufacturing. These processes significantly influenced genomes of played a critical role in improving varieties. In this review, we summarize recent advances genome sequencing, explore impact human-driven selection, recapitulate key genes associated with important agronomic breeding. We provide several examples genomics-guided breeding highlight potential resources facilitating improvement. Furthermore, elaborate progress strategies through design present how such efforts can help future enhancements align demands sustainability evolving societies.

Language: Английский

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Identification of the SWEET gene family and functional characterization of PsSWEET1a and PsSWEET17b in the regulation of sugar accumulation in ‘Fengtang’ plum (Prunus salicina Lindl.)

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 1, 2025

'Fengtang' plum is a cultivar known for its significant sugar accumulation. Although various studies have been conducted on metabolism, the specific molecular mechanisms underlying high accumulation in remain largely unexplored. Here, we present role of Sugars Will Eventually be Exported Transporters (SWEETs) family regulating fruits. In this study, 18 PsSWEET genes were identified based homology with Arabidopsis and Pfam database (ID: PF03083). Alignment multiple sequences revealed that seven alpha-helical transmembrane regions (7-TMs) are conserved family. Phylogenetic analysis demonstrated SWEET members could categorized into four clades. Nine predicted motifs within plum. The unevenly distributed across five chromosomes, synteny three pairs fragmented duplication events. PsSWEET1a PsSWEET17b pivotal fruit development. Subcellular localization analyses indicated localized to nucleus cytoplasm, while associated vacuolar membrane. Gene function was further validated through transient silencing overexpression fruits, which significantly impacted their soluble content. Heterologous expression tomato resulted an increase content due modulation accumulation-related enzyme activities. PsSWEET17b, regulate fruit, successfully identified. This study provides comprehensive insight gene plum, offering novel perspectives regulation laying critical foundation enhancement quality.

Language: Английский

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Starch and sugars as determinants of postharvest shelf life and quality: some new and surprising roles

Current Opinion in Biotechnology, Journal Year: 2022, Volume and Issue: 78, P. 102844 - 102844

Published: Nov. 19, 2022

Starch and sugars account for most of the dry weight horticultural crops in many species, are known determinants quality. However, we posit that these carbohydrates often have less-obvious roles plant tissues with direct implications postharvest quality produce shelf life. The latter has not been given as much attention, but recent interest reducing scale waste loss, highlight how dynamic changes spatial-temporal accumulation carbohydrates, can influence myriads biological processes affecting attributes. Versatile roles, some surprising, play determining high value to consumers, highlighted, gene targets biotechnological improvement specified.

Language: Английский

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Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits

BMC Plant Biology, Journal Year: 2022, Volume and Issue: 22(1)

Published: June 22, 2022

Abstract Background Sugar content is an important indicator of fruit quality. Except for a few wild tomato species that accumulate sucrose in the fruits, most cultivated hexose. Although several studies have focused on sucrose-accumulating tomato, accumulation mechanism still unclear. Results Here, two homozygous inbred cherry lines (‘TB0023’ and ‘TB0278’, which accumulated hexose, respectively) were selected to analyze sugar mechanism. Carbohydrate analysis, cytological observation, gene expression enzyme activity analysis proteomics methods used this study. The results indicated glucose fructose absolutely dominant soluble hexose-accumulating fruit, while certain proportion hexose main forms fruit. phloem unloading pathway switched from symplastic apoplastic during development, probably had mixed involving apoplastic. High acid invertase (AI), phosphate synthase (SPS), (SS) transporters LeSUT1, SlSWEET2a SlSWEET12c factors LeSUT2, SPS, SS, SlSWEET1b, SlSWEET5b, SlSWEET11b, SlSWEET7a, SlSWEET14 responsible solute tomato. Conclusions This study provides detailed evidence elucidation perspective cell structure, physiology molecular biology, providing theoretical basis improvement quality aiding utilization genetic resources.

Language: Английский

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Metabolomic and transcriptomic analyses reveal the mechanism of sweet-acidic taste formation during pineapple fruit development

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 8, 2022

Pineapple (Ananas comosus L.) is one of the most valuable subtropical fruit crop in world. The sweet-acidic taste pineapple fruits a major contributor to characteristic quality, but its formation mechanism remains elusive. Here, targeted metabolomic and transcriptomic analyses were performed during developmental stages two cultivars ("Comte de Paris" "MD-2") gain global view metabolism transport pathways involved sugar organic acid accumulation. Assessment levels different components development revealed that predominant mature both was sucrose citric acid, respectively. Weighted gene coexpression network analysis metabolic phenotypes expression profiling enabled identification 21 genes associated with accumulation 19 coordinated interaction correlated irreversible hydrolysis, resynthesis, could be responsible for fruit. In addition, might controlled by pyruvate-to-acetyl-CoA-to-citrate pathway, gamma-aminobutyric tonoplast proton pumps pineapple. These results provide deep insights into regulation sweetness acidity

Language: Английский

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Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (Punica granatum)

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(5), P. 2471 - 2471

Published: Feb. 23, 2022

Members of the sugars will eventually be exported transporter (SWEET) family regulate transport different through cell membrane and control distribution inside outside cell. The SWEET gene also plays important roles in plant growth development physiological processes. So far, there are no reports on pomegranate. Meanwhile, pomegranate is rich sugar, three published genome sequences provide resources for study family. 20 PgSWEETs from known Arabidopsis grape SWEETs were divided into four clades (Ⅰ, Ⅱ, Ⅲ Ⅳ) according to phylogenetic relationships. same clade share similar structures, predicting their biological functions. RNA-Seq data suggested that PgSWEET genes have a tissue-specific expression pattern. Foliar application tripotassium phosphate significantly increased total soluble sugar content fruits leaves affected levels PgSWEETs. hormone regulator assay both buds bisexual functional male flowers. Among them, we selected PgSWEET17a as candidate role fructose leaves. 798 bp CDS sequence was cloned, which encodes 265 amino acids. subcellular localization showed it localized membrane, indicating its involvement transport. Transient results tobacco with up-regulation PgSWEET17a, while sucrose glucose contents down-regulated. integration tree, structure genome-wide trait Our findings suggest exogenous treatments could alter patterns. These reference further verification metabolism pathway regulation

Language: Английский

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