Hancockiamides: phenylpropanoid piperazines from Aspergillus hancockii are biosynthesised by a versatile dual single-module NRPS pathway DOI
Hang Li, Alastair E. Lacey,

Si Shu

et al.

Organic & Biomolecular Chemistry, Journal Year: 2020, Volume and Issue: 19(3), P. 587 - 595

Published: Nov. 23, 2020

The hancockiamides are an unusual new family of N-cinnamoylated piperazines from the Australian soil fungus Aspergillus hancockii. Genomic analyses A. hancockii identified a biosynthetic gene cluster (hkm) 12 genes, including two single-module nonribosomal peptide synthetase (NRPS) genes. Heterologous expression hkm in nidulans confirmed its role biosynthesis hancockiamides. We further demonstrated that novel cytochrome P450, Hkm5, catalyses methylenedioxy bridge formation, and PAL hkm12 is dispensable, but contributes to increased production cinnamoylated In vitro enzymatic assays substrate feeding studies NRPS Hkm11 activates transfers trans-cinnamate piperazine scaffold has flexibility accept bioisosteric thienyl furyl analogues. This first reported cinnamate-activating fungal NRPS. Expression truncated lacking acetyltransferase led seven additional congeners, unexpected 2,5-dibenzylpiperazines. These pleiotropic effects highlight plasticity pathway power this approach for accessing natural product scaffolds.

Language: Английский

The amazing potential of fungi: 50 ways we can exploit fungi industrially DOI Creative Commons
Kevin D. Hyde, Jianchu Xu, Sylvie Rapior

et al.

Fungal Diversity, Journal Year: 2019, Volume and Issue: 97(1), P. 1 - 136

Published: July 1, 2019

Fungi are an understudied, biotechnologically valuable group of organisms. Due to the immense range habitats that fungi inhabit, and consequent need compete against a diverse array other fungi, bacteria, animals, have developed numerous survival mechanisms. The unique attributes thus herald great promise for their application in biotechnology industry. Moreover, can be grown with relative ease, making production at scale viable. search fungal biodiversity, construction living collection, both incredible economic potential locating organisms novel industrial uses will lead products. This manuscript reviews fifty ways which potentially utilized as biotechnology. We provide notes examples each exploitation give from our own work notable researchers. also flow chart used convince funding bodies importance biotechnological research provided world penicillin, lovastatin, globally significant medicines, they remain untapped resource enormous potential.

Language: Английский

Citations

672

The contribution of fungi to the global economy DOI Creative Commons
Allen Grace T. Niego, Christopher Lambert, Peter E. Mortimer

et al.

Fungal Diversity, Journal Year: 2023, Volume and Issue: 121(1), P. 95 - 137

Published: July 1, 2023

Abstract Fungi provide ecological and environmental services to humans, as well health nutritional benefits, are vital numerous industries. Fermented food beverage products from fungi circulating in the market, generating billions of USD. However, highest potential monetary value is their role blue carbon trading because ability sequester large amounts soil. There no conclusive estimates available on global fungi, primarily there limited data for extrapolation. This study outlines contribution economy provides a first attempt at quantifying fungi. Our estimate USD 54.57 trillion starting point that can be analysed improved, highlighting significance providing an appreciation value. paper identifies different economically valuable provided by By giving all important fungal products, services, industrial applications underscores biodiversity conservation. Furthermore, if established, they will considered future policies effective ecosystem management.

Language: Английский

Citations

100

Naturally Occurring Organohalogen Compounds—A Comprehensive Review DOI
Gordon W. Gribble

Fortschritte der Chemie Organischer Naturstoffe/Fortschritte der Chemie organischer Naturstoffe/Progress in the chemistry of organic natural products, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 546

Published: Jan. 1, 2023

Language: Английский

Citations

50

Current trends, limitations and future research in the fungi? DOI Creative Commons
Kevin D. Hyde, Petr Baldrián, Yanpeng Chen

et al.

Fungal Diversity, Journal Year: 2024, Volume and Issue: 125(1), P. 1 - 71

Published: March 20, 2024

Abstract The field of mycology has grown from an underappreciated subset botany, to a valuable, modern scientific discipline. As this study grown, there have been significant contributions science, technology, and industry, highlighting the value fungi in era. This paper looks at current research, along with existing limitations, suggests future areas where scientists can focus their efforts, mycology. We show how become important emerging diseases medical discuss trends potential drug novel compound discovery. explore phylogenomics, its potential, outcomes address question phylogenomics be applied fungal ecology. In addition, functional genomics studies are discussed importance unravelling intricate mechanisms underlying behaviour, interactions, adaptations, paving way for comprehensive understanding biology. look research building materials, they used as carbon sinks, biocircular economies. numbers always great interest often written about estimates varied greatly. Thus, we needs order obtain more reliable estimates. aspects machine learning (AI) it mycological research. Plant pathogens affecting food production systems on global scale, such, needed area, particularly disease detection. latest data High Throughput Sequencing if still gaining new knowledge same rate before. A review nanotechnology is provided addressed. Arbuscular Mycorrhizal Fungi addressed acknowledged. Fungal databases becoming important, therefore provide major databases. Edible medicinal huge medicines, especially Asia prospects discussed. Lifestyle changes (e.g., endophytes, pathogens, and/or saprobes) also extremely trend special issue Diversity.

Language: Английский

Citations

34

Biological and chemical diversity go hand in hand: Basidiomycota as source of new pharmaceuticals and agrochemicals DOI
Birthe Sandargo, Clara Chepkirui, Tian Cheng

et al.

Biotechnology Advances, Journal Year: 2019, Volume and Issue: 37(6), P. 107344 - 107344

Published: Feb. 10, 2019

Language: Английский

Citations

123

Catalytic Reactions and Energy Conservation in the Cytochrome bc1 and b6f Complexes of Energy-Transducing Membranes DOI Creative Commons
Marcin Sarewicz, Sebastian Pintscher, Rafał Pietras

et al.

Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(4), P. 2020 - 2108

Published: Jan. 19, 2021

This review focuses on key components of respiratory and photosynthetic energy-transduction systems: the cytochrome bc1 b6f (Cytbc1/b6f) membranous multisubunit homodimeric complexes. These remarkable molecular machines catalyze electron transfer from quinones to water-soluble carriers (such as cytochromes c or plastocyanin), coupling flow proton translocation across energy-transducing membrane contributing generation a transmembrane electrochemical potential gradient, which powers cellular metabolism in majority living organisms. Cytsbc1/b6f share many similarities but also have significant differences. While decades research provided extensive knowledge these enzymes, several important aspects their mechanisms remain be elucidated. We summarize broad range structural, mechanistic, physiological required for function Cytbc1/b6f, combining textbook fundamentals with new intriguing concepts that emerged more recent studies. The discussion covers is not limited (i) energy-conserving bifurcation pathway energy-wasting superoxide at quinol oxidation site, (ii) mechanism by semiquinone stabilized quinone reduction (iii) interactions substrates specific inhibitors, (iv) intermonomer role dimeric complex, (v) higher levels organization regulation involve Cytsbc1/b6f. In addressing topics, we point out existing uncertainties controversies, which, suggested, will drive further this field.

Language: Английский

Citations

95

Diels–Alder Reactions During the Biosynthesis of Sorbicillinoids DOI Creative Commons
Lukas Kahlert,

Eman F. Bassiony,

Russell J. Cox

et al.

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 59(14), P. 5816 - 5822

Published: Jan. 13, 2020

The sorbicillinoids are a class of biologically active and structurally diverse fungal polyketides arising from sorbicillin. Through co-expression sorA, sorB, sorC, sorD Trichoderma reesei QM6a, the biosynthetic pathway to epoxysorbicillinol dimeric sorbicillinoids, which resemble Diels-Alder-like Michael-addition-like products, was reconstituted in Aspergillus oryzae NSAR1. Expression feeding experiments demonstrated crucial requirement flavin-dependent monooxygenase SorD for formation hybrid vivo. In contrast prior reports, catalyses neither oxidation 2',3'-dihydrosorbicillin sorbicillin nor sorbicillinol oxosorbicillinol. This is first report that both intermolecular Diels-Alder Michael dimerization reactions, as well epoxidation catalysed vivo by SorD.

Language: Английский

Citations

82

Efficient Reconstitution of Basidiomycota Diterpene Erinacine Gene Cluster in Ascomycota Host Aspergillus oryzae Based on Genomic DNA Sequences DOI
Chengwei Liu, Atsushi Minami, Taro Ozaki

et al.

Journal of the American Chemical Society, Journal Year: 2019, Volume and Issue: 141(39), P. 15519 - 15523

Published: Sept. 19, 2019

To develop the versatile methodology for genome mining of mushroom metabolites, we examined production bioactive diterpenes erinacines using genomic DNA sequences. In this report, initially identified high expression loci (hot spots) in Aspergillus oryzae by sequencing DNAs from highly yielding transformants which were obtained our previous biosynthetic studies. Genome editing knock-in all erinacine genes directly to hot spot showed that A. correctly spliced more than 90% introns gene Then, reconstituted cluster two rounds cDNAs and newly developed repeatable genetic engineering plasmid recycling. At 100% transformation rate, a transformant successfully produced Q its intermediates. study, elucidated pathway involving functionally unique hydroxylation supported dehydrogenase EriH xylose-specific glycosylation introducing plant supplying UDP-xylose. Our recycling allowed us avoid time-consuming screening process use unlimited introduction due marker-free editing.

Language: Английский

Citations

79

Mind the mushroom: natural product biosynthetic genes and enzymes of Basidiomycota DOI
Markus Gressler,

Nikolai A. Löhr,

Tim Schäfer

et al.

Natural Product Reports, Journal Year: 2021, Volume and Issue: 38(4), P. 702 - 722

Published: Jan. 1, 2021

This article comprehensively reviews basidiomycete enzymes and their genes involved in natural product biosynthesis primarily focuses on typical pathways enzymes, along with the methods applied to investigate mushroom metabolism.

Language: Английский

Citations

79

Deciphering chemical logic of fungal natural product biosynthesis through heterologous expression and genome mining DOI
Chen‐Yu Chiang, M. Ohashi, Yi Tang

et al.

Natural Product Reports, Journal Year: 2022, Volume and Issue: 40(1), P. 89 - 127

Published: Sept. 20, 2022

Covering: 2010 to 2022Heterologous expression of natural product biosynthetic gene clusters (BGCs) has become a widely used tool for genome mining cryptic pathways, bottom-up investigation enzymes, and engineered biosynthesis new variants. In the field fungal products, heterologous complete pathway was first demonstrated in tenellin

Language: Английский

Citations

48