Organic & Biomolecular Chemistry,
Journal Year:
2020,
Volume and Issue:
19(3), P. 587 - 595
Published: Nov. 23, 2020
The
hancockiamides
are
an
unusual
new
family
of
N-cinnamoylated
piperazines
from
the
Australian
soil
fungus
Aspergillus
hancockii.
Genomic
analyses
A.
hancockii
identified
a
biosynthetic
gene
cluster
(hkm)
12
genes,
including
two
single-module
nonribosomal
peptide
synthetase
(NRPS)
genes.
Heterologous
expression
hkm
in
nidulans
confirmed
its
role
biosynthesis
hancockiamides.
We
further
demonstrated
that
novel
cytochrome
P450,
Hkm5,
catalyses
methylenedioxy
bridge
formation,
and
PAL
hkm12
is
dispensable,
but
contributes
to
increased
production
cinnamoylated
In
vitro
enzymatic
assays
substrate
feeding
studies
NRPS
Hkm11
activates
transfers
trans-cinnamate
piperazine
scaffold
has
flexibility
accept
bioisosteric
thienyl
furyl
analogues.
This
first
reported
cinnamate-activating
fungal
NRPS.
Expression
truncated
lacking
acetyltransferase
led
seven
additional
congeners,
unexpected
2,5-dibenzylpiperazines.
These
pleiotropic
effects
highlight
plasticity
pathway
power
this
approach
for
accessing
natural
product
scaffolds.
Fungal Diversity,
Journal Year:
2019,
Volume and Issue:
97(1), P. 1 - 136
Published: July 1, 2019
Fungi
are
an
understudied,
biotechnologically
valuable
group
of
organisms.
Due
to
the
immense
range
habitats
that
fungi
inhabit,
and
consequent
need
compete
against
a
diverse
array
other
fungi,
bacteria,
animals,
have
developed
numerous
survival
mechanisms.
The
unique
attributes
thus
herald
great
promise
for
their
application
in
biotechnology
industry.
Moreover,
can
be
grown
with
relative
ease,
making
production
at
scale
viable.
search
fungal
biodiversity,
construction
living
collection,
both
incredible
economic
potential
locating
organisms
novel
industrial
uses
will
lead
products.
This
manuscript
reviews
fifty
ways
which
potentially
utilized
as
biotechnology.
We
provide
notes
examples
each
exploitation
give
from
our
own
work
notable
researchers.
also
flow
chart
used
convince
funding
bodies
importance
biotechnological
research
provided
world
penicillin,
lovastatin,
globally
significant
medicines,
they
remain
untapped
resource
enormous
potential.
Fungal Diversity,
Journal Year:
2023,
Volume and Issue:
121(1), P. 95 - 137
Published: July 1, 2023
Abstract
Fungi
provide
ecological
and
environmental
services
to
humans,
as
well
health
nutritional
benefits,
are
vital
numerous
industries.
Fermented
food
beverage
products
from
fungi
circulating
in
the
market,
generating
billions
of
USD.
However,
highest
potential
monetary
value
is
their
role
blue
carbon
trading
because
ability
sequester
large
amounts
soil.
There
no
conclusive
estimates
available
on
global
fungi,
primarily
there
limited
data
for
extrapolation.
This
study
outlines
contribution
economy
provides
a
first
attempt
at
quantifying
fungi.
Our
estimate
USD
54.57
trillion
starting
point
that
can
be
analysed
improved,
highlighting
significance
providing
an
appreciation
value.
paper
identifies
different
economically
valuable
provided
by
By
giving
all
important
fungal
products,
services,
industrial
applications
underscores
biodiversity
conservation.
Furthermore,
if
established,
they
will
considered
future
policies
effective
ecosystem
management.
Fortschritte der Chemie Organischer Naturstoffe/Fortschritte der Chemie organischer Naturstoffe/Progress in the chemistry of organic natural products,
Journal Year:
2023,
Volume and Issue:
unknown, P. 1 - 546
Fungal Diversity,
Journal Year:
2024,
Volume and Issue:
125(1), P. 1 - 71
Published: March 20, 2024
Abstract
The
field
of
mycology
has
grown
from
an
underappreciated
subset
botany,
to
a
valuable,
modern
scientific
discipline.
As
this
study
grown,
there
have
been
significant
contributions
science,
technology,
and
industry,
highlighting
the
value
fungi
in
era.
This
paper
looks
at
current
research,
along
with
existing
limitations,
suggests
future
areas
where
scientists
can
focus
their
efforts,
mycology.
We
show
how
become
important
emerging
diseases
medical
discuss
trends
potential
drug
novel
compound
discovery.
explore
phylogenomics,
its
potential,
outcomes
address
question
phylogenomics
be
applied
fungal
ecology.
In
addition,
functional
genomics
studies
are
discussed
importance
unravelling
intricate
mechanisms
underlying
behaviour,
interactions,
adaptations,
paving
way
for
comprehensive
understanding
biology.
look
research
building
materials,
they
used
as
carbon
sinks,
biocircular
economies.
numbers
always
great
interest
often
written
about
estimates
varied
greatly.
Thus,
we
needs
order
obtain
more
reliable
estimates.
aspects
machine
learning
(AI)
it
mycological
research.
Plant
pathogens
affecting
food
production
systems
on
global
scale,
such,
needed
area,
particularly
disease
detection.
latest
data
High
Throughput
Sequencing
if
still
gaining
new
knowledge
same
rate
before.
A
review
nanotechnology
is
provided
addressed.
Arbuscular
Mycorrhizal
Fungi
addressed
acknowledged.
Fungal
databases
becoming
important,
therefore
provide
major
databases.
Edible
medicinal
huge
medicines,
especially
Asia
prospects
discussed.
Lifestyle
changes
(e.g.,
endophytes,
pathogens,
and/or
saprobes)
also
extremely
trend
special
issue
Diversity.
Chemical Reviews,
Journal Year:
2021,
Volume and Issue:
121(4), P. 2020 - 2108
Published: Jan. 19, 2021
This
review
focuses
on
key
components
of
respiratory
and
photosynthetic
energy-transduction
systems:
the
cytochrome
bc1
b6f
(Cytbc1/b6f)
membranous
multisubunit
homodimeric
complexes.
These
remarkable
molecular
machines
catalyze
electron
transfer
from
quinones
to
water-soluble
carriers
(such
as
cytochromes
c
or
plastocyanin),
coupling
flow
proton
translocation
across
energy-transducing
membrane
contributing
generation
a
transmembrane
electrochemical
potential
gradient,
which
powers
cellular
metabolism
in
majority
living
organisms.
Cytsbc1/b6f
share
many
similarities
but
also
have
significant
differences.
While
decades
research
provided
extensive
knowledge
these
enzymes,
several
important
aspects
their
mechanisms
remain
be
elucidated.
We
summarize
broad
range
structural,
mechanistic,
physiological
required
for
function
Cytbc1/b6f,
combining
textbook
fundamentals
with
new
intriguing
concepts
that
emerged
more
recent
studies.
The
discussion
covers
is
not
limited
(i)
energy-conserving
bifurcation
pathway
energy-wasting
superoxide
at
quinol
oxidation
site,
(ii)
mechanism
by
semiquinone
stabilized
quinone
reduction
(iii)
interactions
substrates
specific
inhibitors,
(iv)
intermonomer
role
dimeric
complex,
(v)
higher
levels
organization
regulation
involve
Cytsbc1/b6f.
In
addressing
topics,
we
point
out
existing
uncertainties
controversies,
which,
suggested,
will
drive
further
this
field.
Angewandte Chemie International Edition,
Journal Year:
2020,
Volume and Issue:
59(14), P. 5816 - 5822
Published: Jan. 13, 2020
The
sorbicillinoids
are
a
class
of
biologically
active
and
structurally
diverse
fungal
polyketides
arising
from
sorbicillin.
Through
co-expression
sorA,
sorB,
sorC,
sorD
Trichoderma
reesei
QM6a,
the
biosynthetic
pathway
to
epoxysorbicillinol
dimeric
sorbicillinoids,
which
resemble
Diels-Alder-like
Michael-addition-like
products,
was
reconstituted
in
Aspergillus
oryzae
NSAR1.
Expression
feeding
experiments
demonstrated
crucial
requirement
flavin-dependent
monooxygenase
SorD
for
formation
hybrid
vivo.
In
contrast
prior
reports,
catalyses
neither
oxidation
2',3'-dihydrosorbicillin
sorbicillin
nor
sorbicillinol
oxosorbicillinol.
This
is
first
report
that
both
intermolecular
Diels-Alder
Michael
dimerization
reactions,
as
well
epoxidation
catalysed
vivo
by
SorD.
Journal of the American Chemical Society,
Journal Year:
2019,
Volume and Issue:
141(39), P. 15519 - 15523
Published: Sept. 19, 2019
To
develop
the
versatile
methodology
for
genome
mining
of
mushroom
metabolites,
we
examined
production
bioactive
diterpenes
erinacines
using
genomic
DNA
sequences.
In
this
report,
initially
identified
high
expression
loci
(hot
spots)
in
Aspergillus
oryzae
by
sequencing
DNAs
from
highly
yielding
transformants
which
were
obtained
our
previous
biosynthetic
studies.
Genome
editing
knock-in
all
erinacine
genes
directly
to
hot
spot
showed
that
A.
correctly
spliced
more
than
90%
introns
gene
Then,
reconstituted
cluster
two
rounds
cDNAs
and
newly
developed
repeatable
genetic
engineering
plasmid
recycling.
At
100%
transformation
rate,
a
transformant
successfully
produced
Q
its
intermediates.
study,
elucidated
pathway
involving
functionally
unique
hydroxylation
supported
dehydrogenase
EriH
xylose-specific
glycosylation
introducing
plant
supplying
UDP-xylose.
Our
recycling
allowed
us
avoid
time-consuming
screening
process
use
unlimited
introduction
due
marker-free
editing.
Natural Product Reports,
Journal Year:
2021,
Volume and Issue:
38(4), P. 702 - 722
Published: Jan. 1, 2021
This
article
comprehensively
reviews
basidiomycete
enzymes
and
their
genes
involved
in
natural
product
biosynthesis
primarily
focuses
on
typical
pathways
enzymes,
along
with
the
methods
applied
to
investigate
mushroom
metabolism.
Natural Product Reports,
Journal Year:
2022,
Volume and Issue:
40(1), P. 89 - 127
Published: Sept. 20, 2022
Covering:
2010
to
2022Heterologous
expression
of
natural
product
biosynthetic
gene
clusters
(BGCs)
has
become
a
widely
used
tool
for
genome
mining
cryptic
pathways,
bottom-up
investigation
enzymes,
and
engineered
biosynthesis
new
variants.
In
the
field
fungal
products,
heterologous
complete
pathway
was
first
demonstrated
in
tenellin
