Quorum sensing-mediated microbial interactions: Mechanisms, applications, challenges and perspectives

Microbiological Research, Journal Year: 2023, Volume and Issue: 273, P. 127414 - 127414

Published: May 20, 2023

Language: Английский

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Tools for engineering coordinated system behaviour in synthetic microbial consortia

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: July 5, 2018

Abstract Advancing synthetic biology to the multicellular level requires development of multiple cell-to-cell communication channels that propagate information with minimal signal interference. The quorum-sensing devices, cornerstone technology for building microbial communities coordinated system behaviour, has largely focused on cognate acyl-homoserine lactone (AHL)/transcription factor pairs, while use non-cognate pairs as a design feature received limited attention. Here, we demonstrate large library AHL-receiver all and chemical interactions quantified, develop software tool automatically selects orthogonal channels. We this approach identify up four in silico, experimentally simultaneous three co-culture. non-interfering is an enabling step facilitates consortia applications including distributed bio-computation, increased bioprocess efficiency, cell specialisation spatial organisation.

Language: Английский

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Expanding the chemical diversity through microorganisms co-culture: Current status and outlook

Biotechnology Advances, Journal Year: 2020, Volume and Issue: 40, P. 107521 - 107521

Published: Jan. 15, 2020

Language: Английский

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Automated design of synthetic microbial communities

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Jan. 28, 2021

Abstract Microbial species rarely exist in isolation. In naturally occurring microbial systems there is strong evidence for a positive relationship between diversity and productivity of communities. The pervasiveness these communities nature highlights possible advantages genetically engineered strains to cocultures as well. Building synthetic allows us create distributed that mitigate issues often found engineering monoculture, especially functional complexity increases. Here, we demonstrate methodology designing robust include competition nutrients, use quorum sensing control amensal bacteriocin interactions chemostat environment. We computationally explore all two- three- strain systems, using Bayesian methods perform model selection, identify the most candidates producing stable steady state Our findings highlight important interaction motifs provide stability, requirements selecting genetic parts further tuning community composition.

Language: Английский

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Synthetic Biology for Manipulating Quorum Sensing in Microbial Consortia

Trends in Microbiology, Journal Year: 2020, Volume and Issue: 28(8), P. 633 - 643

Published: April 24, 2020

Language: Английский

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Transcription-Factor-based Biosensor Engineering for Applications in Synthetic Biology

ACS Synthetic Biology, Journal Year: 2021, Volume and Issue: 10(5), P. 911 - 922

Published: April 25, 2021

Transcription-factor-based biosensors (TFBs) are often used for metabolite detection, adaptive evolution, and metabolic flux control. However, designing TFBs with superior performance applications in synthetic biology remains challenging. Specifically, natural do not meet real-time detection requirements owing to their slow response times inappropriate dynamic ranges, sensitivity, selectivity. Furthermore, optimizing complex regulation networks is time-consuming labor-intensive. This Review highlights TFB-based recent engineering strategies ranging from traditional trial-and-error approaches novel computer-model-based rational design approaches. The limitations of the these additionally reviewed.

Language: Английский

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De novo design of an intercellular signaling toolbox for multi-channel cell–cell communication and biological computation

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Aug. 24, 2020

Intercellular signaling is indispensable for single cells to form complex biological structures, such as biofilms, tissues and organs. The genetic tools available engineering intercellular signaling, however, are quite limited. Here we exploit the chemical diversity of small molecules de novo design a toolbox high-performance, multi-channel cell-cell communications computations. By biosynthetic pathway signal molecules, rational sensing promoters directed evolution transcription factors, obtain six channels in bacteria with orthogonality far exceeding conventional quorum systems successfully transfer some them into yeast human cells. For demonstration, they applied cell consortia generate bacterial colony-patterns using up four simultaneously implement distributed bio-computation containing seven different strains basic units. This paves way multicellularity including artificial ecosystems smart tissues.

Language: Английский

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Bioelectronic control of a microbial community using surface-assembled electrogenetic cells to route signals

Nature Nanotechnology, Journal Year: 2021, Volume and Issue: 16(6), P. 688 - 697

Published: March 29, 2021

Language: Английский

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Inducible cell-to-cell signaling for tunable dynamics in microbial communities

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: March 4, 2020

Abstract The last decade has seen bacteria at the forefront of biotechnological innovation, with applications including biomolecular computing, living therapeutics, microbiome engineering and microbial factories. These emerging are all united by need to precisely control complex dynamics in spatially extended environments, requiring tools that can bridge gap between intracellular population-level coordination. To address this need, we engineer an inducible quorum sensing system which enables precise tunability bacterial both population community level. As a proof-of-principle, demonstrate advantages when genetically equipped for cargo delivery. In addition, exploit absence cross-talk respect majority well-characterized systems inducibility multi-strain communities. More broadly, work highlights unexplored potential remotely which, coupled any gene interest, may facilitate translation circuit designs into applications.

Language: Английский

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Quorum Sensing Communication: Molecularly Connecting Cells, Their Neighbors, and Even Devices

Annual Review of Chemical and Biomolecular Engineering, Journal Year: 2020, Volume and Issue: 11(1), P. 447 - 468

Published: March 14, 2020

Quorum sensing (QS) is a molecular signaling modality that mediates molecular-based cell-cell communication. Prevalent in nature, QS networks provide bacteria with method to gather information from the environment and make decisions based on intel. With its ability autonomously facilitate both inter- intraspecies gene regulation, this process can be rewired enable actuated, but molecularly programmed, genetic control. On one hand, novel QS-based circuits endow cells smart functions used many fields of engineering, other, repurposed circuitry promotes communication aids development synthetic microbial consortia. Furthermore, engineered systems probe intervene interkingdom between their hosts. Lastly, demonstrated establish conversation abiotic materials, especially by taking advantage biological even electronically induced assembly processes; such QS-incorporated biohybrid devices offer innovative ways program cell behavior function.

Language: Английский

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