3D Printed Spectroelectrochemical Platform for Redox‐Based Bioelectronics DOI Creative Commons
Chen‐Yu Chen, Eunkyoung Kim, Fauziah Rahma Zakaria

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Redox provides unique opportunities for interconverting molecular/biological information into electronic signals. Here, the fabrication of a 3D‐printed multiwell device that can be interfaced existing laboratory instruments (e.g., well‐plate readers and microscopes) to enable advanced redox‐based spectral electrochemical capabilities is reported. In first application, mediated probing used as soft sensing method biomanufacturing: it shown signal metrics discern intact mAbs from partially reduced mAb variants (fragmentation), these near‐real‐time electrical measurements correlate off‐line chemical analysis. second operando spectroelectrochemical are characterize redox‐active catechol‐based hydrogel film: electron transfer into/from film correlates molecular switching film's redox state with absorbance increasing upon oxidation fluorescence reduction. final example, synthetic biofilm containing redox‐responsive E. coli electro‐assembled: gene expression induced under reducing conditions (via reductive H 2 O generation) or oxidative phenolic redox‐signaling molecule). Overall, this work demonstrates 3D printing allows bespoke devices accelerate understanding phenomena in biology detection/characterization activities technology.

Language: Английский

Real-time bioelectronic sensing of environmental contaminants DOI
Joshua T. Atkinson, Lin Su, Xu Zhang

et al.

Nature, Journal Year: 2022, Volume and Issue: 611(7936), P. 548 - 553

Published: Nov. 2, 2022

Language: Английский

Citations

137
Engineered Living Materials For Sustainability DOI
Bolin An, Yan‐Yi Wang, Yuan‐Yuan Huang

et al.

Chemical Reviews, Journal Year: 2022, Volume and Issue: 123(5), P. 2349 - 2419

Published: Dec. 13, 2022

Recent advances in synthetic biology and materials science have given rise to a new form of materials, namely engineered living (ELMs), which are composed matter or cell communities embedded self-regenerating matrices their own artificial scaffolds. Like natural such as bone, wood, skin, ELMs, possess the functional capabilities organisms, can grow, self-organize, self-repair when needed. They also spontaneously perform programmed biological functions upon sensing external cues. Currently, ELMs show promise for green energy production, bioremediation, disease treatment, fabricating advanced smart materials. This review first introduces dynamic features systems potential developing novel We then summarize recent research progress on emerging design strategies from both perspectives. Finally, we discuss positive impacts promoting sustainability key future directions.

Language: Английский

Citations

120
An electrogenetic interface to program mammalian gene expression by direct current DOI Creative Commons
Jinbo Huang, Shuai Xue, P. Buchmann

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(8), P. 1395 - 1407

Published: July 31, 2023

Wearable electronic devices are playing a rapidly expanding role in the acquisition of individuals' health data for personalized medical interventions; however, wearables cannot yet directly program gene-based therapies because lack direct electrogenetic interface. Here we provide missing link by developing an interface that call current (DC)-actuated regulation technology (DART), which enables electrode-mediated, time- and voltage-dependent transgene expression human cells using DC from batteries. DART utilizes supply to generate non-toxic levels reactive oxygen species act via biosensor reversibly fine-tune synthetic promoters. In proof-of-concept study type 1 diabetic male mouse model, once-daily transdermal stimulation subcutaneously implanted microencapsulated engineered energized acupuncture needles (4.5 V 10 s) stimulated insulin release restored normoglycemia. We believe this will enable wearable metabolic interventions.

Language: Английский

Citations

48
Active biointegrated living electronics for managing inflammation DOI
Jiuyun Shi,

Saehyun Kim,

Pengju Li

et al.

Science, Journal Year: 2024, Volume and Issue: 384(6699), P. 1023 - 1030

Published: May 30, 2024

Seamless interfaces between electronic devices and biological tissues stand to revolutionize disease diagnosis treatment. However, biomechanical disparities synthetic materials living present challenges at bioelectrical signal transduction interfaces. We introduce the active biointegrated electronics (ABLE) platform, encompassing capabilities across biogenic, biomechanical, properties simultaneously. The biointerface, comprising a bioelectronics layout

Language: Английский

Citations

43
Synthetic microbiology in sustainability applications DOI
Ethan Jones, John P. Marken, Pamela A. Silver

et al.

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(6), P. 345 - 359

Published: Jan. 22, 2024

Language: Английский

Citations

19
Integrating bioelectronics with cell-based synthetic biology DOI
Jonathan Rivnay, Ritu Raman, Jacob T. Robinson

et al.

Nature Reviews Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Language: Английский

Citations

4
Engineering Wired Life: Synthetic Biology for Electroactive Bacteria DOI
Lina J. Bird, Biki Bapi Kundu, Tanya Tschirhart

et al.

ACS Synthetic Biology, Journal Year: 2021, Volume and Issue: 10(11), P. 2808 - 2823

Published: Oct. 12, 2021

Electroactive bacteria produce or consume electrical current by moving electrons to and from extracellular acceptors donors. This specialized process, known as electron transfer, relies on pathways composed of redox active proteins biomolecules has enabled technologies ranging harvesting energy the sea floor, chemical sensing, carbon capture. Harnessing controlling transfer using bioengineering synthetic biology promises heighten limits established open doors new possibilities. In this review, we provide an overview recent advancements in genetic tools for manipulating native electroactive control transfer. After reviewing natively organisms, examine lessons learned introduction into Escherichia coli. We conclude presenting challenges future efforts give examples opportunities bioengineer microbes electrochemical applications.

Language: Английский

Citations

76
Dynamic cybergenetic control of bacterial co-culture composition via optogenetic feedback DOI Creative Commons
Joaquı́n Gutiérrez, Sant Kumar, Mustafa Khammash

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 16, 2022

Communities of microbes play important roles in natural environments and hold great potential for deploying division-of-labor strategies synthetic biology bioproduction. However, the difficulty controlling composition microbial consortia over time hinders their optimal use many applications. Here, we present a fully automated, high-throughput platform that combines real-time measurements computer-controlled optogenetic modulation bacterial growth to implement precise robust compositional control two-strain E. coli community. In addition, develop general framework dynamic modeling genetic circuits physiological context host-aware model determine parameters our closed-loop system. Our succeeds stabilizing strain ratio multiple parallel co-cultures at arbitrary levels changing these targets time, opening door implementation programs communities.

Language: Английский

Citations

57
Biomimetic Wearable Sensors: Emerging Combination of Intelligence and Electronics DOI Creative Commons

Donglei Pan,

Jiawang Hu,

Bin Wang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 11(5)

Published: Dec. 3, 2023

Abstract Owing to the advancement of interdisciplinary concepts, for example, wearable electronics, bioelectronics, and intelligent sensing, during microelectronics industrial revolution, nowadays, extensively mature sensing devices have become new favorites in noninvasive human healthcare industry. The combination with bionics is driving frontier developments various fields, such as personalized medical monitoring flexible due superior biocompatibilities diverse mechanisms. It noticed that integration desired functions into device materials can be realized by grafting biomimetic intelligence. Therefore, herein, mechanism which satisfy further enhance system functionality reviewed. Next, artificial sensory systems integrate portable are introduced, received significant attention from industry owing their novel approaches portabilities. To address limitations encountered important signal data units systems, two paths forward identified current challenges opportunities presented this field. In summary, review provides a comprehensive understanding development both breadth depth perspectives, offering valuable guidance future research application expansion these devices.

Language: Английский

Citations

34
Living Synthelectronics: A New Era for Bioelectronics Powered by Synthetic Biology DOI
Jing Sun, Ruofan Yang, Qingsong Li

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(25)

Published: March 17, 2024

Abstract Bioelectronics, which converges biology and electronics, has attracted great attention due to their vital applications in human–machine interfaces. While traditional bioelectronic devices utilize nonliving organic and/or inorganic materials achieve flexibility stretchability, a biological mismatch is often encountered because human tissues are characterized not only by softness stretchability but also biodynamic adaptive properties. Recently, notable paradigm shift emerged bioelectronics, where living cells, even viruses, modified via gene editing within synthetic biology, used as core components new hybrid electronics paradigm. These defined “living synthelectronics,” they offer enhanced potential for interfacing with at informational substance exchange levels. In this Perspective, the recent advances synthelectronics summarized. First, opportunities brought briefly introduced. Then, strategic approaches designing making electronic using cells/viruses building blocks, sensing components, or power sources reviewed. Finally, challenges faced raised. It believed that will significantly contribute real integration of bioelectronics tissues.

Language: Английский

Citations

9