Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering DOI Creative Commons
Yuan Ma, Xinhui Wang, Ting Su

et al.

Gels, Journal Year: 2022, Volume and Issue: 8(10), P. 606 - 606

Published: Sept. 21, 2022

Hydrogels have been extensively used as scaffolds in tissue engineering for cell adhesion, proliferation, migration, and differentiation because of their high-water content biocompatibility similarity to the extracellular matrix. However, submicron or nanosized pore networks within hydrogels severely limit survival regeneration. In recent years, application macroporous has received considerable attention. The structure not only facilitates nutrient transportation metabolite discharge but also provides more space behavior formation. Several strategies creating functionalizing reported. This review began with an overview advantages challenges regulation cellular behavior. addition, advanced methods preparation modulate were discussed. Finally, future research related fields was

Language: Английский

Piezo1‐mediated M2 macrophage mechanotransduction enhances bone formation through secretion and activation of transforming growth factor‐β1 DOI Creative Commons

Guanhui Cai,

Yahui Lu,

Weijie Zhong

et al.

Cell Proliferation, Journal Year: 2023, Volume and Issue: 56(9)

Published: March 7, 2023

Macrophages are multifunctional immune system cells that essential for the mechanical stimulation-induced control of metabolism. Piezo1 is a non-selective calcium channel expressed in multifarious tissues to convey signals. Here, cellular model tension was used study effect stretch on phenotypic transformation macrophages and its mechanism. An indirect co-culture explore macrophage activation bone marrow mesenchymal stem (BMSCs), treadmill running validate mechanism vivo vitro studies. p53 acetylated deacetylated by as result strain being detected Piezo1. This process able polarize towards M2 secretes transforming growth factor-beta (TGF-β1), which subsequently stimulates BMSCs migration, proliferation osteogenic differentiation. Knockdown inhibits conversion reparative phenotype, thereby affecting remodelling. Blockade TGF-β I, II receptors significantly reduced exercise-increased mass mice. In conclusion, we showed causes influx, deacetylation, polarization TGF-β1 release through These events support BMSC osteogenesis.

Language: Английский

Citations

45
Hydrogel crosslinking modulates macrophages, fibroblasts, and their communication, during wound healing DOI Creative Commons
Sergei Butenko, Raji R. Nagalla, Christian F. Guerrero‐Juarez

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 9, 2024

Biomaterial wound dressings, such as hydrogels, interact with host cells to regulate tissue repair. This study investigates how crosslinking of gelatin-based hydrogels influences immune and stromal cell behavior healing in female mice. We observe that softer, lightly crosslinked promote greater cellular infiltration result smaller scars compared stiffer, heavily hydrogels. Using single-cell RNA sequencing, we further show increase inflammation lead the formation a distinct macrophage subpopulation exhibiting signs oxidative activity fusion. Conversely, are more readily taken up by macrophages integrated within tissue. The physical properties differentially affect fibroblast interactions, promoting pro-fibrotic drives fusion through RANKL signaling. These findings suggest tuning can guide responses improve healing, offering insights for designing better biomaterials treatment.

Language: Английский

Citations

41
Mechanisms of mechanotransduction and physiological roles of PIEZO channels DOI
Bailong Xiao

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Language: Английский

Citations

30
Evidence and therapeutic implications of biomechanically regulated immunosurveillance in cancer and other diseases DOI
Vincent Mittelheisser, Valentin Gensbittel, Lucia Bonati

et al.

Nature Nanotechnology, Journal Year: 2024, Volume and Issue: 19(3), P. 281 - 297

Published: Jan. 29, 2024

Language: Английский

Citations

28
Mechanosensing regulates tissue repair program in macrophages DOI Creative Commons
Matthew L. Meizlish, Yoshitaka Kimura, Scott D. Pope

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(11)

Published: March 13, 2024

Tissue-resident macrophages play important roles in tissue homeostasis and repair. However, how monitor maintain integrity is not well understood. The extracellular matrix (ECM) a key structural organizational component of all tissues. Here, we find that sense the mechanical properties ECM to regulate specific repair program. We show macrophage mechanosensing mediated by cytoskeletal remodeling can be performed three-dimensional environments through noncanonical, integrin-independent mechanism analogous amoeboid migration. these dynamics also integrate biochemical signaling colony-stimulating factor 1 ultimately chromatin accessibility control mechanosensitive gene expression This study identifies an "amoeboid" mode which may fibrosis.

Language: Английский

Citations

23
Matrix stiffness affects tumor-associated macrophage functional polarization and its potential in tumor therapy DOI Creative Commons
Jiaqiang Xiong, Rourou Xiao, Jiahui Zhao

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 21, 2024

The extracellular matrix (ECM) plays critical roles in cytoskeletal support, biomechanical transduction and biochemical signal transformation. Tumor-associated macrophage (TAM) function is regulated by stiffness solid tumors often associated with poor prognosis. ECM stiffness-induced mechanical cues can activate cell membrane mechanoreceptors corresponding mechanotransducers the cytoplasm, modulating phenotype of TAMs. Currently, tuning TAM polarization through stimulation has received increasing attention, whereas its effect on fate rarely been summarized. A better understanding relationship between will contribute to development new strategies for cancer therapy. In this review, we first introduced overall stiffness, analyzed changes mediated tumor progression, finally summarized effects targeting prognosis provide insight into field.

Language: Английский

Citations

20
Decoding the “Fingerprint” of Implant Materials: Insights into the Foreign Body Reaction DOI
Yangmengfan Chen,

Zeyu Luo,

Weikun Meng

et al.

Small, Journal Year: 2024, Volume and Issue: 20(23)

Published: Jan. 8, 2024

Abstract Foreign body reaction (FBR) is a prevalent yet often overlooked pathological phenomenon, particularly within the field of biomedical implantation. The presence FBR poses heavy burden on both medical and socioeconomic systems. This review seeks to elucidate protein “fingerprint” implant materials, which generated by physiochemical properties materials themselves. In this review, activity macrophages, formation foreign giant cells (FBGCs), development fibrosis capsules in context are introduced. Additionally, relationship between various elucidated detail, as an overview existing approaches technologies employed alleviate FBR. Finally, significance components (metallic non‐metallic materials), surface CHEMISTRY (charge wettability), physical characteristics (topography, roughness, stiffness) establishing also well documented. conclusion, aims emphasize importance provides current perspectives developing with anti‐FBR properties.

Language: Английский

Citations

19
PIEZO Ion Channels in Cardiovascular Functions and Diseases DOI
Bertrand Coste, Patrick Delmas

Circulation Research, Journal Year: 2024, Volume and Issue: 134(5), P. 572 - 591

Published: Feb. 29, 2024

The cardiovascular system provides blood supply throughout the body and as such is perpetually applying mechanical forces to cells tissues. Thus, this primed with mechanosensory structures that respond adapt changes in stimuli. Since their discovery 2010, PIEZO ion channels have dominated field of mechanobiology. These been proposed long-sought-after mechanosensitive excitatory involved touch proprioception mammals. However, more pieces evidence point importance activities disease development. channel-related cardiac functions include transducing hemodynamic endothelial vascular cells, red cell homeostasis, platelet aggregation, arterial pressure regulation, among others. contribute pathological conditions including hypertrophy pulmonary hypertension congenital syndromes generalized lymphatic dysplasia xerocytosis. In review, we highlight recent advances understanding role diseases. Achievements quickly expanding should open a new road for efficient control PIEZO-related diseases functions.

Language: Английский

Citations

19
PIEZO1 mechanically regulates the antitumour cytotoxicity of T lymphocytes DOI

Ruiyang Pang,

Weihao Sun, Yingyun Yang

et al.

Nature Biomedical Engineering, Journal Year: 2024, Volume and Issue: 8(9), P. 1162 - 1176

Published: March 21, 2024

Language: Английский

Citations

18
Biomaterial-based mechanical regulation facilitates scarless wound healing with functional skin appendage regeneration DOI Creative Commons
Yingying Li,

Shuaifei Ji,

Xiaobing Fu

et al.

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: Feb. 18, 2024

Abstract Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss appendages, ultimately impairing its normal physiological function. Accumulating evidence underscores potential targeted modulation mechanical cues enhance regeneration, promoting scarless repair by influencing extracellular microenvironment driving phenotypic transitions. The field appendage regeneration has witnessed remarkable advancements in utilization biomaterials with distinct physical properties. However, a comprehensive understanding underlying mechanisms remains somewhat elusive, limiting broader application these innovations. In this review, we present two promising biomaterial-based approaches aimed at bolstering regenerative capacity compromised skin. first approach involves leveraging specific biophysical properties create an optimal environment that supports cellular activities essential for regeneration. second centers on harnessing forces exerted plasticity, facilitating efficient reprogramming and, consequently, appendages. summary, manipulation using strategies holds significant promise as supplementary achieving wound healing, coupled restoration multiple functions.

Language: Английский

Citations

17