Interaction between immuno-stem dual lineages in jaw bone formation and injury repair DOI Creative Commons
Ziyi Liu,

Luo Xu-tao,

Ruoshi Xu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: March 6, 2024

The jawbone, a unique structure in the human body, undergoes faster remodeling than other bones due to presence of stem cells and its distinct immune microenvironment. Long-term exposure jawbones an oral environment rich microbes results complex balance, as shown by higher proportion activated macrophage jaw. Stem derived from jawbone have propensity differentiate into osteoblasts those bones. microenvironment jaw also promotes osteogenic differentiation cells. Here, we summarize various types involved reconstruction. We describe mechanism relationship between cells, including through production inflammatory bodies, secretion cytokines, activation signaling pathways, etc. In addition, comb out cellular interaction within under development, homeostasis maintenance pathological conditions. This review aims eclucidate uniqueness context cell microenvironment, hopefully advancing clinical regeneration jawbone.

Language: Английский

Macrophage Polarization and the Regulation of Bone Immunity in Bone Homeostasis DOI Creative Commons

Kangyi Hu,

Zhengya Shang,

Xiaorui Yang

et al.

Journal of Inflammation Research, Journal Year: 2023, Volume and Issue: Volume 16, P. 3563 - 3580

Published: Aug. 1, 2023

Abstract: Bone homeostasis is a dynamic equilibrium state of bone formation and absorption, ensuring skeletal development repair. immunity encompasses all aspects the intersection between immune systems, including various signaling pathways, cytokines, crosstalk cells under both homeostatic pathological conditions. Therefore, as key cell types in immunity, macrophages can polarize into classical pro-inflammatory M1 alternative anti-inflammatory M2 influence body environment, participating regulation metabolism playing roles homeostasis. not only act precursors osteoclasts (OCs), differentiate mature OCs, but also secrete cytokines to promote resorption; while osteogenic factors, stimulating differentiation mineralization osteoblast mesenchymal stem (MSCs), subsequently increase formation. Once polarization imbalanced, resulting dysregulation will cause inflammatory stimulation, release large amount factors affecting metabolism, leading conditions such osteoporosis (OP), rheumatoid arthritis (RA), steroid-induced femoral head necrosis (SANFH). In this review, we introduce pathways related macrophage polarization, well their relationships with OB, OC, MSC. We discuss diseases imbalance, regulating them, which may help develop new methods for treating metabolic disorders. Keywords: macrophage,

Language: Английский

Citations

51
Piezo1‐mediated M2 macrophage mechanotransduction enhances bone formation through secretion and activation of transforming growth factor‐β1 DOI Creative Commons

Guanhui Cai,

Yahui Lu,

Weijie Zhong

et al.

Cell Proliferation, Journal Year: 2023, Volume and Issue: 56(9)

Published: March 7, 2023

Macrophages are multifunctional immune system cells that essential for the mechanical stimulation-induced control of metabolism. Piezo1 is a non-selective calcium channel expressed in multifarious tissues to convey signals. Here, cellular model tension was used study effect stretch on phenotypic transformation macrophages and its mechanism. An indirect co-culture explore macrophage activation bone marrow mesenchymal stem (BMSCs), treadmill running validate mechanism vivo vitro studies. p53 acetylated deacetylated by as result strain being detected Piezo1. This process able polarize towards M2 secretes transforming growth factor-beta (TGF-β1), which subsequently stimulates BMSCs migration, proliferation osteogenic differentiation. Knockdown inhibits conversion reparative phenotype, thereby affecting remodelling. Blockade TGF-β I, II receptors significantly reduced exercise-increased mass mice. In conclusion, we showed causes influx, deacetylation, polarization TGF-β1 release through These events support BMSC osteogenesis.

Language: Английский

Citations

43
Macrophage plasticity: signaling pathways, tissue repair, and regeneration DOI Creative Commons
Lingfeng Yan, Jue Wang,

Xin Cai

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: Aug. 1, 2024

Abstract Macrophages are versatile immune cells with remarkable plasticity, enabling them to adapt diverse tissue microenvironments and perform various functions. Traditionally categorized into classically activated (M1) alternatively (M2) phenotypes, recent advances have revealed a spectrum of macrophage activation states that extend beyond this dichotomy. The complex interplay signaling pathways, transcriptional regulators, epigenetic modifications orchestrates polarization, allowing respond stimuli dynamically. Here, we provide comprehensive overview the cascades governing focusing on roles Toll‐like receptors, signal transducer activator transcription proteins, nuclear microRNAs. We also discuss emerging concepts metabolic reprogramming trained immunity, contributing their functional adaptability. Macrophage plasticity plays pivotal role in repair regeneration, macrophages coordinating inflammation, angiogenesis, matrix remodeling restore homeostasis. By harnessing potential novel therapeutic strategies targeting polarization could be developed for diseases, including chronic wounds, fibrotic disorders, inflammatory conditions. Ultimately, deeper understanding molecular mechanisms underpinning will pave way innovative regenerative medicine engineering approaches.

Language: Английский

Citations

28
Matrix stiffness affects tumor-associated macrophage functional polarization and its potential in tumor therapy DOI Creative Commons
Jiaqiang Xiong, Rourou Xiao, Jiahui Zhao

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 21, 2024

The extracellular matrix (ECM) plays critical roles in cytoskeletal support, biomechanical transduction and biochemical signal transformation. Tumor-associated macrophage (TAM) function is regulated by stiffness solid tumors often associated with poor prognosis. ECM stiffness-induced mechanical cues can activate cell membrane mechanoreceptors corresponding mechanotransducers the cytoplasm, modulating phenotype of TAMs. Currently, tuning TAM polarization through stimulation has received increasing attention, whereas its effect on fate rarely been summarized. A better understanding relationship between will contribute to development new strategies for cancer therapy. In this review, we first introduced overall stiffness, analyzed changes mediated tumor progression, finally summarized effects targeting prognosis provide insight into field.

Language: Английский

Citations

20
Macrophage-mediated immunomodulation in biomaterial-assisted bone repair: Molecular insights and therapeutic prospects DOI Creative Commons
Zhiguo Bi,

Yimeng Cai,

Xiaotong Shi

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 488, P. 150631 - 150631

Published: March 26, 2024

The skeletal system is essential for preserving body structure and facilitating mobility; however, bone diseases frequently result in significant disabilities, necessitating the investigation into biomaterials restoration. Nevertheless, these may elicit immune reactions that obstruct healing process. Macrophages assume a pivotal role modulating inflammation sustaining homeostasis regeneration. A comprehensive understanding of molecular interactions between macrophages imperative development sophisticated designed to regulate environment promote remodeling. This review delves generation, polarization, interaction with principal cell types implicated osteogenesis, detailing mechanisms by which influence repair their response biomaterials. Additionally, it discusses strategies precise modulation macrophage functionality via intelligent mechanisms, outlines challenges constraints designing integrate functions. By elucidating challenges, lays groundwork creation advanced endowed smart immunomodulatory capabilities, heralding novel approaches treatment defects advancement regeneration therapies.

Language: Английский

Citations

17
Piezo1 Enhances Macrophage Phagocytosis and Pyrin Activation to Ameliorate Fungal Keratitis DOI Creative Commons
Jiahui Yang, Jing Zhong, Zhenyuan Fu

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(1), P. 33 - 33

Published: Jan. 14, 2025

Fungal keratitis (FK) remains a treatment challenge, necessitating new therapeutic targets. Piezo1, mechanosensitive ion channel, regulates calcium signaling and immune cell function. This study investigates its role in macrophage-mediated antifungal responses FK. Piezo1 Pyrin expression corneas bone marrow-derived macrophages (BMDMs) were assessed by RNAseq, quantitative real-time PCR (qRT-PCR), Western blot, immunofluorescence. Intracellular concentration was detected Fluo-4 AM fluorescent probe staining. Heterozygous deficiency (Piezo1+/-) mice Yoda1 performed to regulate the of Piezo1. Our investigation demonstrates elevated patients with FK infected mice. upregulation corresponded swift recruitment via limbus. Additionally, Piezo1+/- exacerbate progression infection model. Furthermore, knockdown exhibit notable reduction phagocytic capacity, accompanied an increase viable colony-forming units vitro model fungal infection. Moreover, using pharmacologic activator (Yoda1), (Ca2+) chelator BAPTA or mice, we demonstrate that activation triggers inflammasome augmented influx, which is required for protection against murine hosts. crucial innate immunity FK, enhancing macrophage recruitment, activation, inflammasome-mediated activity signaling. Using Yoda1, confirm Piezo1's clearance. Targeting offers novel strategy improve outcomes boosting function response.

Language: Английский

Citations

1
Static Topographical Cue Combined with Dynamic Fluid Stimulation Enhances the Macrophage Extracellular Vesicle Yield and Therapeutic Potential for Bone Defects DOI

Huayi Huang,

Lingfei Xiao,

Lucheng Fang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Extracellular vesicles (EVs) hold promise for tissue regeneration, but their low yield and limited therapeutic efficacy hinder clinical translation. Bioreactors provide a larger culture surface area stable environment large-scale EV production, yet ability to enhance is limited. Physical stimulation, by inducing cell differentiation modulating cargo composition, offers more efficient, cost-effective, reproducible approach compared the loading of EVs biochemical priming parental cells. Herein, effects 3D-printed perfusion bioreactor with topographical cue on macrophage bioactivity were assessed. The results indicate that increased 12.5-fold enhanced in promoting osteogenic angiogenesis via upregulated miR-210-3p. Mechanistically, fluid shear stress activates Piezo1, triggering Ca2+ influx Yes-associated protein (YAP) nuclear translocation, secretion enhancing M2 polarization conjunction morphological changes guided aligned topography. Moreover, porous electrospun membrane-hydrogel composite scaffold loaded bioreactor-derived exhibited outstanding rat cranial defect model. This study presents scalable, platform production EVs, potentially overcoming key challenges translating EV-based therapies clinic.

Language: Английский

Citations

1
Macrophages in graft-versus-host disease (GVHD): dual roles as therapeutic tools and targets DOI Creative Commons
Atieh Raoufi, Hamed Soleimani Samarkhazan, S Nouri

et al.

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 6, 2025

Graft-versus-host disease remains one of the most formidable barriers to complete success hematopoietic stem cell transplantation that has emerged as curative approach for many malignancies because it affects quality life and overall survival. Macrophages are among important members immune system, which perform dual roles in GVHD both therapeutic tools targets. This review epitomizes multifunctional role macrophages pathophysiology acute chronic GVHD. play an early phase their recruitment infiltration into target organs. Furthermore, they polarize two functionally different phenotypes, including M1 M2. In case GVHD, express phenotype characterized by production pro-inflammatory cytokines contribute tissue damage. contrast, tend toward M2 associated with repair tissues fibrosis. A critical balance these phenotypes is central course severity Further interactions other lymphocytes such T cells, B fibroblast further determine Macrophage interaction alloreactive cells promotes inflammation. therefore inducing injuries during Interaction macrophages, cell, fibroblast, CD4+ fibrosis and, hence, subsequent dysfunction These some insights, while several challenges remain. First, impact dominant on polarization incompletely sometimes controversial. Second, development targeted therapies able modulate macrophage function without systemic side effects area ongoing investigation. Future directions involve exploration macrophage-targeted therapies, small molecules, antibodies, nanotechnology, behavior improve patient outcomes. underlines fact a profound understanding essential developing new more effective strategies. Targeting might represent avenue decreasing incidence improving safety HSCT.

Language: Английский

Citations

1
Bioactive Phosphorus Dendrimers Deliver Protein/Drug to Tackle Osteoarthritis via Cooperative Macrophage Reprogramming DOI
Huxiao Sun, Mengsi Zhan, Yu Zou

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 316, P. 122999 - 122999

Published: Dec. 5, 2024

Language: Английский

Citations

6
The emerging role of Piezo1 in the musculoskeletal system and disease DOI Creative Commons
Lei Lei,

Zhenkang Wen,

Mingde Cao

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(10), P. 3963 - 3983

Published: Jan. 1, 2024

Piezo1, a mechanosensitive ion channel, has emerged as key player in translating mechanical stimuli into biological signaling. Its involvement extends beyond physiological and pathological processes such lymphatic vessel development, axon growth, vascular immunoregulation, blood pressure regulation. The musculoskeletal system, responsible for structural support, movement, homeostasis, recently attracted attention regarding the significance of Piezo1. This review aims to provide comprehensive summary current research on Piezo1 highlighting its impact bone formation, myogenesis, chondrogenesis, intervertebral disc tendon matrix cross-linking, physical activity. Additionally, we explore potential targeting therapeutic approach disorders, including osteoporosis, muscle atrophy, degeneration, osteoarthritis.

Language: Английский

Citations

5