Atypical Centriolar Composition Correlates with Internal Fertilization in Fish DOI Creative Commons
Katerina Turner, Nisha Solanki, Hassan O. Salouha

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(5), P. 758 - 758

Published: Feb. 22, 2022

The sperm competition theory, as proposed by Geoff Parker, predicts that evolve through a cascade of changes. As an example, internal fertilization is followed morphology diversification. However, little known about the evolution structures. centriole has ancient and evolutionarily conserved canonical structure with signature 9-fold, radially symmetric microtubules form cell’s centrosomes, cilia, flagella. Most animal spermatozoa have two centrioles, one which forms spermatozoan flagellum. Both are delivered to egg constitute embryo’s first centrosomes. mammals insects only recognizable structure. A second atypical was recently reported in both groups which, prior this, eluded discovery standard techniques criteria. Because ancestors reproduced fertilization, we hypothesized transition from centrioles composition ancestral centriolar characterized evolved preferentially after fertilization. We examined fish because diversity species available test this hypothesis–as some reproduce via others external fertilization–and their ultrastructure been extensively studied. Our literature search reports on 277 species. Species specifically enriched among fertilizers compared (7/34, 20.6% versus 2/243, 0.80%; p < 0.00001, odds ratio = 32.4) represent phylogenetically unrelated fish. Atypical present subfamily Poeciliinae. Therefore, internally fertilizing independently multiple times, agreeing Parker’s theory.

Language: Английский

Human sperm rotate with a conserved direction during free swimming in four dimensions DOI Creative Commons
Gabriel Corkidi, Fernando Montoya, Ana Laura González‐Cota

et al.

Journal of Cell Science, Journal Year: 2023, Volume and Issue: 136(22)

Published: Oct. 30, 2023

Head rotation in human spermatozoa is essential for different swimming modes and fertilisation, as it links the molecular workings of flagellar beat with sperm motion three-dimensional (3D) space over time. Determining direction head has been hindered by symmetry translucent nature head, fast 3D driven helical beat. Analysis mostly restricted to two-dimensional (2D) single focal plane image analysis, which enables tracking centre position but not rotation. Despite conserved beating flagellum, reported be uni- or bi-directional, even intermittently change a given cell. Here, we directly measure freely using multi-plane 4D (3D+t) microscopy show that: (1) 2D unable distinguish spermatozoa; (2) non-capacitating capacitating solutions, both aqueous viscous media, counterclockwise (CCW), seen from tail, all rotating spermatozoa, regardless experimental conditions; (3) suppressed 36% medium, although CCW recovered after incubation conditions within same possibly unveiling an unexplored aspect need capacitation fertilisation. Our observations that conserved. It constitutes robust persistent driving mechanism influences navigation time, thus critical importance cell motility, propulsion flagellated microorganisms, motility assessments, reproduction research, self-organisation patterns space.

Language: Английский

Citations

7
Cilia structure and function in human disease DOI Creative Commons
Gregory J. Pazour

Current Opinion in Endocrine and Metabolic Research, Journal Year: 2024, Volume and Issue: 34, P. 100509 - 100509

Published: Feb. 20, 2024

Language: Английский

Citations

2
CEP112 coordinates translational regulation of essential fertility genes during spermiogenesis through phase separation in humans and mice DOI Creative Commons
Xueguang Zhang, Gelin Huang,

Ting Jiang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 30, 2024

Language: Английский

Citations

2
Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality DOI Creative Commons
Katerina Turner, Emily Fishman,

Mariam Asadullah

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: April 22, 2021

A large proportion of infertility and miscarriage causes are unknown. One potential cause is a defective sperm centriole, subcellular structure essential for motility embryonic development. Yet, the extent to which centriolar maladies contribute male unknown due lack convenient way assess centriole quality. We developed robust, location-based, ratiometric assay overcome this roadblock, Fluorescence-based Ratiometric Assessment Centrioles (FRAC). performed case series study with semen samples from 33 patients, separated using differential gradient centrifugation into higher-grade (pellet) lower-grade (interface) fractions. Using reference population infertile men morphologically standard sperm, we found that 79% substandard morphology have suboptimal centrioles (P = 0.0005). Moreover, tubulin labeling distal correlates negatively age 0.004, R -0.66). These findings suggest FRAC sensitive method patient associated

Language: Английский

Citations

15
Atypical Centriolar Composition Correlates with Internal Fertilization in Fish DOI Creative Commons
Katerina Turner, Nisha Solanki, Hassan O. Salouha

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(5), P. 758 - 758

Published: Feb. 22, 2022

The sperm competition theory, as proposed by Geoff Parker, predicts that evolve through a cascade of changes. As an example, internal fertilization is followed morphology diversification. However, little known about the evolution structures. centriole has ancient and evolutionarily conserved canonical structure with signature 9-fold, radially symmetric microtubules form cell’s centrosomes, cilia, flagella. Most animal spermatozoa have two centrioles, one which forms spermatozoan flagellum. Both are delivered to egg constitute embryo’s first centrosomes. mammals insects only recognizable structure. A second atypical was recently reported in both groups which, prior this, eluded discovery standard techniques criteria. Because ancestors reproduced fertilization, we hypothesized transition from centrioles composition ancestral centriolar characterized evolved preferentially after fertilization. We examined fish because diversity species available test this hypothesis–as some reproduce via others external fertilization–and their ultrastructure been extensively studied. Our literature search reports on 277 species. Species specifically enriched among fertilizers compared (7/34, 20.6% versus 2/243, 0.80%; p < 0.00001, odds ratio = 32.4) represent phylogenetically unrelated fish. Atypical present subfamily Poeciliinae. Therefore, internally fertilizing independently multiple times, agreeing Parker’s theory.

Language: Английский

Citations

9