m6A readers ECT2/ECT3/ECT4 enhance mRNA stability through direct recruitment of the poly(A) binding proteins in Arabidopsis

Genome biology, Journal Year: 2023, Volume and Issue: 24(1)

Published: April 30, 2023

Abstract Background RNA N 6 -methyladenosine (m A) modification is critical for plant growth and crop yield. m A reader proteins can recognize modifications to facilitate the functions of in gene regulation. ECT2, ECT3, ECT4 are readers that known redundantly regulate trichome branching leaf growth, but their molecular remain unclear. Results Here, we show directly interact with each other cytoplasm perform genetically redundant abscisic acid (ABA) response regulation during seed germination post-germination growth. We reveal ECT2/ECT3/ECT4 promote stabilization targeted A-modified mRNAs, have no function alternative polyadenylation translation. find ECT2 interacts poly(A) binding proteins, PAB2 PAB4, maintains mRNAs. Disruption destabilizes mRNAs ABA signaling-related genes, thereby promoting accumulation ABI5 leading hypersensitivity. Conclusion Our study reveals a unified functional model mediated by plants. In this model, target cytoplasm.

Language: Английский

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The m6A reader ECT8 is an abiotic stress sensor that accelerates mRNA decay in Arabidopsis

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(8), P. 2908 - 2926

Published: June 5, 2024

Abstract N 6-methyladenosine (m6A) is the most abundant mRNA modification and plays diverse roles in eukaryotes, including plants. It regulates various processes, plant growth, development, responses to external or internal stress responses. However, mechanisms underlying how m6A related environmental stresses both mammals plants remain elusive. Here, we identified EVOLUTIONARILY CONSERVED C-TERMINAL REGION 8 (ECT8) as an reader protein showed that its m6A-binding capability required for salt Arabidopsis (Arabidopsis thaliana). ECT8 accelerates degradation of target transcripts through direct interaction with decapping DECAPPING 5 within processing bodies. We observed a significant increase expression level under stresses. Using representative stressor, found transcript levels rise response stress. The increased abundance results enhanced binding m6A-modified mRNAs, thereby accelerating their degradation, especially those negative regulators Our demonstrated acts abiotic sensor, facilitating decay, which vital maintaining transcriptome homeostasis enhancing tolerance findings not only advance understanding epitranscriptomic gene regulation but also offer potential applications breeding more resilient crops face rapidly changing conditions.

Language: Английский

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Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis

Nature Plants, Journal Year: 2023, Volume and Issue: 9(12), P. 2042 - 2058

Published: Dec. 8, 2023

Abstract Light regulates chlorophyll homeostasis and photosynthesis via various molecular mechanisms in plants. The light regulation of transcription protein stability nuclear-encoded chloroplast proteins have been extensively studied, but how mRNA metabolism affects abundance remains poorly understood. Here we show that the blue receptor cryptochrome 2 (CRY2) METTL16-type m 6 A writer FIONA1 (FIO1) regulate response to light. In contrast CRY2-mediated photo-condensation adenosine methylase (MTA), photoexcited CRY2 co-condenses FIO1 only presence CRY2-signalling SUPPRESSOR PHYTOCHROME (SPA1). SPA1 synergistically or additively activate RNA methyltransferase activity vitro, whereas FIO1, not MTA, are required for light-induced methylation translation mRNAs encoding multiple regulators vivo. Our study demonstrates liquid–liquid phase separation photoreceptor/writer complexes is commonly involved photoresponsive changes methylation, different CRY/FIO1 CRY/MTA explain, at least partially, writer-specific functions plant photomorphogenesis.

Language: Английский

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Strategies for adaptation to high light in plants

aBIOTECH, Journal Year: 2024, Volume and Issue: 5(3), P. 381 - 393

Published: May 13, 2024

Abstract Plants absorb light energy for photosynthesis via photosystem complexes in their chloroplasts. However, excess can damage the photosystems and decrease photosynthetic output, thereby inhibiting plant growth development. have developed a series of acclimation strategies that allow them to withstand high light. In first line defense against light, leaves chloroplasts move away from accumulates compounds filter reflect second defense, known as photoprotection, plants dissipate through non-photochemical quenching, cyclic electron transport, photorespiration, scavenging reactive oxygen species. third which occurs after photodamage, initiate cycle (mainly II) repair. addition being site photosynthesis, sense stress, especially transduce stress signal nucleus, where it modulates expression genes involved response. this review, we discuss current progress our understanding mechanisms employed by at whole-plant, cellular, physiological, molecular levels across three lines defense.

Language: Английский

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Quantitative profiling of m6A at single base resolution across the life cycle of rice and Arabidopsis

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 7, 2024

Abstract N 6 -methyladenosine (m A) plays critical roles in regulating mRNA metabolism. However, comprehensive m A methylomes different plant tissues with single-base precision have yet to be reported. Here, we present transcriptome-wide maps at resolution of rice and Arabidopsis using A-SAC-seq. Our analysis uncovers a total 205,691 sites distributed across 22,574 genes rice, 188,282 19,984 . The evolutionarily conserved ortholog gene pairs are involved controlling tissue development, photosynthesis stress response. We observe an overall stabilization effect by 3’ UTR certain tissues. Like mammals, positive correlation between the level length internal exons is also observed mRNA, except for last exon. data suggest active deposition process occurring near stop codon mRNA. In addition, MTA-installed correlate both translation promotion suppression, depicting more complicated regulatory picture. results therefore provide in-depth resources relating functions plants uncover suppression-activation model biogenesis species.

Language: Английский

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VvHY5 and VvBEE1 antagonistically control resveratrol biosynthesis to mitigate high light‐induced damage in grapevine

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

ABSTRACT Excessive exposure to high light can lead photoinhibition, which impairs photosynthetic efficiency and causes oxidative damage in plants, such as sunburn grapevines. This study investigates the role of resveratrol (Res), a stilbenoid with antioxidant properties, protecting plants from damage. We found that increased reactive oxygen species (ROS) accumulation induced photoinhibition grapevine leaves. In response, Res biosynthesis was upregulated, along an increase stilbene synthase ( VvSTS ) expression. Application exogenous alleviated ROS improved efficiency. Further analysis revealed VvHY5‐VvBEE1 regulatory module plays pivotal regulating expression under conditions. Specifically, VvHY5 activated expression, while VvBEE1 repressed it. Transgenic showed overexpression enhanced production photoprotection, whereas reduced levels exacerbated light‐induced competed for binding promoter, brassinosteroids (BRs) modulating their interaction. Our findings reveal interplay between signaling brassinosteroid pathways biosynthesis, providing insights grapevines sunburn.

Language: Английский

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Principles, functions, and biological implications of m⁶A in plants

RNA, Journal Year: 2024, Volume and Issue: 30(5), P. 491 - 499

Published: March 26, 2024

Over the past decade, N 6 -methyladenosine (m A) has emerged as a prevalent and dynamically regulated modification across transcriptome; it been reversibly installed, removed, interpreted by specific binding proteins, played crucial roles in molecular biological processes. Within this scope, we consolidate recent advancements of m A research plants regarding gene expression regulation, diverse physiologic pathogenic processes, well crop trial implications, to guide discussions on challenges associated with leveraging epitranscriptome editing for improvement.

Language: Английский

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Tools and Techniques Used at Global Scale Through Genomics, Transcriptomics, Proteomics, and Metabolomics to Investigate Plant Stress Responses at the Molecular Level

Published: Jan. 1, 2024

Language: Английский

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The plant cytosolic m6A RNA methylome stabilizes photosynthesis in the cold

Plant Communications, Journal Year: 2023, Volume and Issue: 4(6), P. 100634 - 100634

Published: June 7, 2023

The sessile lifestyle of plants requires an immediate response to environmental stressors that affect photosynthesis, growth, and crop yield. Here, we showed three abiotic perturbations-heat, cold, high light-triggered considerable changes in the expression signatures 42 epitranscriptomic factors (writers, erasers, readers) with putative chloroplast-associated functions formed clusters commonly expressed genes Arabidopsis. under all conditions were reversible upon deacclimation, identifying players as modulators acclimation processes. Chloroplast dysfunctions, particularly those induced by oxidative stress-inducing norflurazon a largely GENOME UNCOUPLED-independent manner, triggered retrograde signals remodel patterns. N6-methyladenosine (m6A) is known most prevalent RNA modification impacts numerous developmental physiological living organisms. During cold treatment, components primary nuclear m6A methyltransferase complex was upregulated, accompanied significant increase cellular mRNA marks. In presence FIP37, core component writer complex, played important role positive regulation thylakoid structure, photosynthetic functions, accumulation photosystem I, Cytb6f cyclic electron transport proteins, Curvature Thylakoid1 but not II chloroplast ATP synthase. Downregulation FIP37 affected abundance, polysomal loading, translation cytosolic transcripts related photosynthesis suggesting m6A-dependent translational functions. summary, identified multifaceted roles methylome coping cold; these predominantly associated chloroplasts served stabilize photosynthesis.

Language: Английский

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Downregulation of GhALKBH10B improves drought tolerance through increasing the stability of photosynthesis related- and ABA signaling pathway genes in cotton

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 220, P. 105687 - 105687

Published: Feb. 7, 2024

Language: Английский

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