Single-cell sequencing to multi-omics: technologies and applications

Biomarker Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Sept. 27, 2024

Abstract Cells, as the fundamental units of life, contain multidimensional spatiotemporal information. Single-cell RNA sequencing (scRNA-seq) is revolutionizing biomedical science by analyzing cellular state and intercellular heterogeneity. Undoubtedly, single-cell transcriptomics has emerged one most vibrant research fields today. With optimization innovation technologies, intricate details concealed within cells are gradually unveiled. The combination scRNA-seq other multi-omics at forefront field. This involves simultaneously measuring various omics data individual cells, expanding our understanding across a broader spectrum dimensions. precisely captures aspects transcriptomes, immune repertoire, spatial information, temporal epitopes, in diverse contexts. In addition to depicting cell atlas normal or diseased tissues, it also provides cornerstone for studying differentiation development patterns, disease heterogeneity, drug resistance mechanisms, treatment strategies. Herein, we review traditional technologies outline latest advancements multi-omics. We summarize current status challenges applying biological clinical applications. Finally, discuss limitations potential strategies address them.

Language: Английский

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Exploring the Microbial Landscape: Gut Dysbiosis and Therapeutic Strategies in Pancreatitis—A Narrative Review

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 645 - 645

Published: March 14, 2024

The gut microbiota is emerging as an important contributor to the homeostasis of human body through its involvement in nutrition and metabolism, protection against pathogens, development modulation immune system. It has therefore become research topic recent decades. Although association between intestinal dysbiosis numerous digestive pathologies been thoroughly researched, pancreatic diseases constitutes a novelty specialized literature. In years, growing evidence pointed critical pancreas regulating microbiota, well impact on physiology, which implies existence bidirectional connection known "gut-pancreas axis". theorized that any change at either these levels triggers response other component, hence leading evolution pancreatitis. However, there are not enough data determine whether underlying cause or result pancreatitis; therefore, more needed this area. purpose narrative review highlight role pathogenesis acute chronic pancreatitis, evolution, prospect employing therapeutic intervention for

Language: Английский

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Multifunctional Boron‐based 2D Nanoplatforms Ameliorate Severe Respiratory Inflammation by Targeting Multiple Inflammatory Mediators

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Effective management of serious respiratory diseases, such as asthma and recalcitrant rhinitis, remains a global challenge. Here, it is shown that induced sputum supernatants (ISS) from patients with contain higher levels cell-free DNA (cfDNA) compared to healthy volunteers. Although cfDNA scavenging strategies have been developed for inflammation modulation in previous studies, this fall short clinical settings due the excessive neutrophil extracellular trap (NET) formation, reactive oxygen nitrogen species (RONS) bacterial infections injured airway tissues. Based on this, multifunctional boron-based 2D nanoplatform B-PM designed by coating boron nanosheets (B-NS) polyamidoamine generation 1 (PG1) dendrimer, which can simultaneously target cfDNA, NETs, RONS, bacteria. The effects promoting mucosal repair, reducing inflammation, mucus production demonstrated model mice, therapeutic effect superior dexamethasone. Furthermore, flow cytometry clustering analysis transcriptome RNA-sequencing are adopted comprehensively evaluate vivo anti-inflammation effects. These findings emphasize significance multi-targeting strategy modulate dysregulated highlight nanoplatforms amelioration inflammatory diseases.

Language: Английский

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Unpacking the sepsis controversy

Trauma Surgery & Acute Care Open, Journal Year: 2025, Volume and Issue: 10(1), P. e001733 - e001733

Published: March 1, 2025

Despite its many definitions and revisions, consensus statements clinical guidelines, the term 'sepsis' continues to be referred as a discrete entity that is often claimed direct cause of mortality. The assertion sepsis can defined 'life-threatening organ dysfunction caused by dysregulated host response infection,' has led field dominated failed trials informed host-centered, pathogen-agnostic, animal experiments in which models do not recapitulate condition. observations from National Health Service England claim 77.5% deaths occur those aged 75 years or older USA indicating most patients dying have also been diagnosed with 'hospice qualifying conditions,' seem refute by, rather than associated with, 'dysregulated response.' This piece challenges current conceptual framework forms basis definition. Here we posit result both definition use inappropriate models, ineffective treatments continue pursued this field.

Language: Английский

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The Interaction of Microbiome and Pancreas in Acute Pancreatitis

Biomolecules, Journal Year: 2023, Volume and Issue: 14(1), P. 59 - 59

Published: Dec. 31, 2023

Acute pancreatitis (AP) is a common acute abdomen disease characterized by the pathological activation of digestive enzymes and self-digestion pancreatic acinar cells. Secondary infection sepsis are independent prognosticators for AP progression increased mortality. Accumulating anatomical epidemiological evidence suggests that dysbiosis gut microbiota affects etiology severity through intestinal barrier disruption, local or systemic inflammatory response, bacterial translocation, regulatory role microbial metabolites in patients animal models. Recent studies discussing interactions between pancreas have opened new scopes AP, therapeutic interventions target bacteria community received substantial attention. This review concentrates on alterations its roles modulating gut–pancreas axis AP. The potential therapies targeting microbes as well major challenges applying those explored. We expect to understand diagnosis treatment.

Language: Английский

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The gut microbiota in thrombosis

Nature Reviews Cardiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

Language: Английский

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Association between nonalcholic fatty liver disease and pancreatic cancer: Epidemiology, mechanisms, and antidiabetic medication

Hepatology Research, Journal Year: 2024, Volume and Issue: 54(8), P. 729 - 735

Published: June 17, 2024

Extrahepatic malignancies are the leading cause of death in patients with nonalcoholic fatty liver disease (NAFLD). Of these cancers, pancreatic cancer is one most lethal; however, link between NAFLD and remains unclear. Recently, various research results have been reported on association cancer, compiling this information revealed following. First, prevalence at 0.26%. Second, currently evident pathogenesis includes intrapancreatic risk factors, such as: (1) non-alcoholic pancreas disease, (2) intraductal papillary mucinous neoplasm; extrapancreatic insulin resistance adipocytokines, proinflammatory cytokines, (3) dysbiosis. Finally, metformin sodium-glucose cotransporter 2 inhibitors may reduce diabetes NAFLD. In review, we summarize recent evidence epidemiology mechanisms for NAFLD-related cancer. We further discuss impact anti-diabetic medication

Language: Английский

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The role and mechanism of gut-lung axis mediated bidirectional communication in the occurrence and development of chronic obstructive pulmonary disease

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Oct. 24, 2024

The current studies have shown that the occurrence and development of chronic obstructive pulmonary disease (COPD) are closely related to changes in gut health its microenvironment, even some diseases significant clinical correlation with COPD. dysbiosis microbiota observed COPD patients also suggests a potential bidirectional interaction between lung. Communication lung may occur through circulating inflammatory cells, microbial metabolites, mediators, but mechanism communication is still under study. Therefore, more research needed this area. In review, we summarize recent animal models on role gut-lung axis mechanisms, so as provide ideas for further field. addition, summarized negative effects medication risk factors proposed prevention treatment strategies.

Language: Английский

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Impact of gut microbial diversity on egg production performance in chickens

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

ABSTRACT Chickens are one of the most economically important poultry species, and their egg-laying performance is a crucial economic trait. The intestinal microbiome plays significant role in performance. To clarify diversity chicken microbiota its connection to performance, this study utilized 16S rRNA sequencing technology characterize microbiomes 101 chickens from 13 breeds with varying levels egg production. results reveal differences gut structure among groups production levels. High egg-producing showed significantly higher abundances Firmicutes , Proteobacteria Lactobacillus while low displayed greater microbial α-diversity more complex community structures. These influence key physiological functions, including nutrient absorption hormone regulation through metabolic pathways, directly affect medium partially overlapped on principal coordinates analysis plot, whereas high-production group was distinctly separate. This provides scientific basis data for selecting probiotics related high chickens. IMPORTANCE elucidates critical chickens, indicator industry. By employing sequencing, we uncovered distinct profiles associated exhibit abundance specific bacterial taxa, such as which linked enhanced efficiency. Conversely, lower display diversity, suggesting but less efficient ecosystem. Our findings provide valuable insights into relationship between production, offering foundation selection that could potentially improve research not only advances our understanding avian microbiology also has practical implications optimizing farming practices enhancing outcomes.

Language: Английский

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Neutrophils: Linking Inflammation to Thrombosis and Unlocking New Treatment Horizons

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1965 - 1965

Published: Feb. 25, 2025

Neutrophils play a key role in inflammatory responses and thrombosis, but their complex interactions disease pathogenesis are not fully understood. This review examines the multifaceted roles of neutrophils, focusing on activation, cytokine release, formation neutrophil extracellular traps (NETs), which contribute to host defense thrombosis. We discuss interaction between inflammation coagulation, direct effect neutrophils thrombus stability, involvement pathological thrombotic diseases. The therapeutic potential drug loading treatment as well clinical implications future research directions, highlighted. aim this is gain insight into critical neutrophil-inflammation-thrombus axis its target for diseases suggest possible directions neutrophil-loaded therapy

Language: Английский

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