High Light-Driven Simultaneous Expression of Genes Involved in Tca Cycle and Photosynthetic Pathway Reduces the Accumulation of Succinic Acid in a Medicinal Plant Pinellia Ternata DOI

Li-Lin Gao,

Yi Dong,

Zhu Cun

et al.

Published: Jan. 1, 2024

Language: Английский

Non-conventional yeasts: promising cell factories for organic acid bioproduction DOI
Yupeng Wang, Yi Wang, Jie Cui

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Facilitated Channeling of Fixed Carbon and Energy into Chemicals in Artificial Phototrophic Communities DOI
Chaofeng Li, Haotian Zheng, Yuhang Li

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Light-driven CO2 biovalorization offers a promising route for coupling carbon mitigation with petrochemical replacement. Synthetic phototrophic communities that mimic lichens can reduce the metabolic burden improved utilization. However, inefficient channeling of and energy between species seriously hinders collaborative CO2-to-molecule route. Herein, we report universal sequestration (UCS) module based on photosynthetic microbes provides high-speed tunnel to heterotrophs. Compared traditional CO2-to-sucrose module, UCS sequestered 30% more into glycerol, generally available source high density. We demonstrated be highly compatible various industrial chassis genetically recalcitrant microbes, enabling rapid development synthetic without additional genetic manipulation. Notably, accelerated electron transport nutrient recycling systems may facilitate communications cooperative partners. These module-based efficiently channeled wide range chemicals, negative footprint -25.04 -440.74 kgCO2e/kg products. This strategy widens boundaries artificial boost carbon-negative biomanufacturing.

Language: Английский

Citations

1
Issatchenkia orientalis as a platform organism for cost-effective production of organic acids DOI Creative Commons
Shih‐I Tan, Zijun Liu, Vinh Tran

et al.

Metabolic Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

1
Engineering Corynebacterium glutamicum cell factory for producing biochemicals DOI Creative Commons

Kai Li,

Menglin Sun,

Bing Yuan

et al.

BioDesign Research, Journal Year: 2025, Volume and Issue: unknown, P. 100008 - 100008

Published: Feb. 1, 2025

Language: Английский

Citations

1
Multidimensional combinatorial screening for high-level production of erythritol in Yarrowia lipolytica DOI
Lei Li, Qianqian Zhang,

Ruirui Shi

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 406, P. 131035 - 131035

Published: June 24, 2024

Language: Английский

Citations

4
Boosting succinic acid production of Yarrowia lipolytica at low pH through enhancing product tolerance and glucose metabolism DOI Creative Commons

Yutao Zhong,

Changyu Shang,

Huilin Tao

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Oct. 24, 2024

Succinic acid (SA) is an important bio-based C4 platform chemical with versatile applications, including the production of 1,4-butanediol, tetrahydrofuran, and γ-butyrolactone. The non-conventional yeast Yarrowia lipolytica has garnered substantial interest as a robust cell factory for SA at low pH. However, high concentrations SA, especially under acidic conditions, can impose significant stress on microbial cells, leading to reduced glucose metabolism viability compromised performance. Therefore, it develop Y. strains enhanced tolerance industrial-scale production. An SA-tolerant strain E501 improved was obtained through adaptive laboratory evolution (ALE). In 5-L bioreactor, evolved produced 89.62 g/L representing 7.2% increase over starting Hi-SA2. Genome resequencing transcriptome analysis identified mutation in 26S proteasome regulatory subunit Rpn1, well genes involved transmembrane transport, which may be associated tolerance. By further fine-tuning glycolytic pathway flux, highest titer 112.54 date pH achieved, yield 0.67 g/g productivity 2.08 g/L/h. This study provided engineered capable efficiently producing pH, thereby reducing cost industrial fermentation.

Language: Английский

Citations

4
Progress on production of malic acid and succinic acid by industrially-important engineered microorganisms DOI
Na Wu, Wenxin Wang, Jian-Guo Zhu

et al.

Journal of Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Engineering Yarrowia lipolytica to Enhance the Production of Malonic Acid via Malonyl‐CoA Pathway at High Titer DOI Creative Commons

Qun Yang,

Mengzhen Tian,

Pingsha Dong

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Malonic acid (MA) is a high-value-added chemical with significant applications in the polymers, pharmaceutical, and food industries. Microbial production of MA presents enzyme inefficiencies, competitive metabolic pathways, dispersive carbon flux, which collectively limit its biosynthesis. Here, non-conventional oleaginous yeast Yarrowia lipolytica genetically engineered to enhance production. Initially, malonyl-CoA pathway, comprising hydrolase from Saccharomyces cerevisiae, confirmed as most efficient for Y. lipolytica. To further production, two novel hydrolases exhibiting higher activity than S. are identified Fusarium oxysporum, respectively. The introduction F. oxysporum increases titer 6.3 g L-1. Subsequently, advanced engineering strategies performed ensure sufficient flux precursors acetyl-CoA resulting 13.8 L-1 shaking-flasks. Finally, by employing fermentation conditions feeding strategies, maximum concentration 63.6 achieved at 156 h productivity 0.41 h-1 fed-batch fermentation. This study provides new way high titer.

Language: Английский

Citations

0
Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica DOI
Jianhui Liu,

Yamin Zhu,

Jin Hou

et al.

Engineering Microbiology, Journal Year: 2025, Volume and Issue: 5(2), P. 100193 - 100193

Published: March 18, 2025

Language: Английский

Citations

0
Recent advances in biological synthesis of food additive succinate DOI
Qiang Ding,

Mengqi Ji,

Buhan Yao

et al.

Critical Reviews in Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: March 19, 2025

Succinate, a crucial bio-based chemical building block, has already found extensive applications in fields such as food additives, pharmaceutical intermediates, and the materials industry. To efficiently economically synthesize succinate, substantial endeavors have been executed to optimize fermentation processes downstream operations. Nonetheless, there is still need enhance cost-effectiveness competitiveness while considering environmental concerns, particularly light of escalating demands challenges posed by global warming. This article primarily focuses on application metabolic engineering strategies strengthen succinate biosynthesis. These encompass regulation, cellular model guidance. By leveraging advanced synthetic biology techniques, this review highlights potential for developing robust microbial cell factories shaping future directions integration microbes industrial applications.

Language: Английский

Citations

0