Divergent amplifications of CYP9A cytochrome P450 genes provide two noctuid pests with differential protection against xenobiotics DOI Creative Commons
Yu Shi, Qingqing Liu, Wenjie Lu

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(37)

Published: Sept. 5, 2023

Here, we provide mechanistic support for the involvement of CYP9A subfamily cytochrome P450 monooxygenases in detoxification host plant defense compounds and chemical insecticides Spodoptera exigua frugiperda . Our comparative genomics shows that a large cluster genes occurs two species but with significant differences its contents, including several species-specific duplicates substantial sequence divergence, both between orthologs duplicates. Bioassays CRISPR-Cas9 knockouts clusters show that, collectively, CYP9As can detoxify furanocoumarin (imperatorin xanthotoxin) representing three different chemotypes (pyrethroids, avermectins, oxadiazines). However, vitro metabolic assays heterologously expressed products individual particular activities against these compounds. We also find tight genetic linkage high levels pyrethroid resistance field strains species. propose their divergent amplifications have not only contributed to development broad ranges over long evolutionary timeframes supplied them diverse options evolving very recent past.

Language: Английский

High-quality genome assembly of the silkworm, Bombyx mori DOI Creative Commons
Munetaka Kawamoto, Akiya Jouraku, Atsushi Toyoda

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2019, Volume and Issue: 107, P. 53 - 62

Published: Feb. 23, 2019

In 2008, the genome assembly and gene models for domestic silkworm, Bombyx mori, were published by a Japanese Chinese collaboration group. However, contains non-negligible number of misassembled gap regions due to presence many repetitive sequences within silkworm genome. The erroneous occasionally causes incorrect prediction. Here we performed hybrid based on 140 × deep sequencing long (PacBio) short (Illumina) reads. remaining gaps in initial closed using BAC Fosmid sequences, giving new total length 460.3 Mb, with 30 an N50 comprising 16.8 Mb scaffolds 12.2 contigs. More RNA-seq piRNA-seq reads mapped compared previous version, indicating that covers more transcribed regions, including elements. We prediction available mRNA protein sequence data. was 16,880 2154 bp. reflected accurate coding sets than old ones. proportion elements also reestimated assembly, calculated be 46.8% are provided SilkBase (http://silkbase.ab.a.u-tokyo.ac.jp).

Language: Английский

Citations

255
Advances in organophosphorus pesticides pollution: Current status and challenges in ecotoxicological, sustainable agriculture, and degradation strategies DOI

Huiyang Fu,

Peng Tan, R. Wang

et al.

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 424, P. 127494 - 127494

Published: Oct. 14, 2021

Language: Английский

Citations

232
A chromosome-level genome of the spider Trichonephila antipodiana reveals the genetic basis of its polyphagy and evidence of an ancient whole-genome duplication event DOI Creative Commons
Zheng Fan, Tao Yuan, Piao Liu

et al.

GigaScience, Journal Year: 2021, Volume and Issue: 10(3)

Published: March 1, 2021

Abstract Background The spider Trichonephila antipodiana (Araneidae), commonly known as the batik golden web spider, preys on arthropods with body sizes ranging from ∼2 mm in length to insects larger than itself (>20‒50 mm), indicating its polyphagy and strong dietary detoxification abilities. Although it has been reported that an ancient whole-genome duplication event occurred spiders, lack of a high-quality genome limited characterization this event. Results We present chromosome-level T. constructed basis PacBio Hi-C sequencing. assembled is 2.29 Gb size scaffold N50 172.89 Mb. scaffolding assigned 98.5% bases 13 pseudo-chromosomes, BUSCO completeness analysis revealed assembly included 94.8% complete arthropod universal single-copy orthologs (n = 1,066). Repetitive elements account for 59.21% genome. predicted 19,001 protein-coding genes, which 96.78% were supported by transcriptome-based evidence 96.32% matched protein records UniProt database. also shows substantial expansions several detoxification-associated gene families, including cytochrome P450 mono-oxygenases, carboxyl/cholinesterases, glutathione-S-transferases, ATP-binding cassette transporters, reflecting possible genomic polyphagy. Further architecture reveals event, based 2 lines evidence: (i) large-scale duplications inter-chromosome synteny (ii) duplicated clusters Hox genes. Conclusions represents valuable resource research provides insights into species’ adaptation environment.

Language: Английский

Citations

228
The role of cytochrome P450-mediated detoxification in insect adaptation to xenobiotics DOI
Kai Lǚ, Yuanyuan Song, Rensen Zeng

et al.

Current Opinion in Insect Science, Journal Year: 2020, Volume and Issue: 43, P. 103 - 107

Published: Dec. 30, 2020

Language: Английский

Citations

223
Invasiveness, biology, ecology, and management of the fall armyworm, Spodoptera frugiperda DOI
Marc Kenis, Giovanni Benelli, Antonio Biondi

et al.

Entomologia Generalis, Journal Year: 2022, Volume and Issue: 43(2), P. 187 - 241

Published: Oct. 26, 2022

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Citations

220
Biology, invasion and management of the agricultural invader: Fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) DOI Creative Commons
Jing Wan, Cong Huang, Changyou Li

et al.

Journal of Integrative Agriculture, Journal Year: 2021, Volume and Issue: 20(3), P. 646 - 663

Published: Feb. 16, 2021

The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), is native to the Americas. It has rapidly invaded 47 African countries and 18 Asian since first detection of invasion into Nigeria Ghana in 2016. regarded as a super pest based on its host range (at least 353 plants), inherent ability survive wide habitats, strong migration ability, high fecundity, rapid development resistance insecticides/viruses gluttonous characteristics. inherently superior biological characteristics FAW contribute invasiveness. Integrated management (IPM) relied multiple applications monitoring scouting, agricultural control, chemical pesticides, viral insecticides, sex attractants, bio-control agents (parasitoids, predators entomopathogens) botanicals. Knowledge gaps remain be filled to: (1) understand invasive mechanisms S. frugiperda; (2) how prevent further spread (3) provide better strategies. This review summarizes characters FAW, their association with invasiveness IPM strategies, which may insights for future management.

Language: Английский

Citations

164
Resistance in the Genus Spodoptera: Key Insect Detoxification Genes DOI Creative Commons
Frédérique Hilliou, Thomas Chertemps, Martine Maı̈bèche

et al.

Insects, Journal Year: 2021, Volume and Issue: 12(6), P. 544 - 544

Published: June 11, 2021

The genus Spodoptera (Lepidoptera: Noctuidae) includes species that are among the most important crop pests in world. These polyphagous able to feed on many plants, including corn, rice and cotton. In addition their ability adapt toxic compounds produced by they have developed resistance chemical insecticides used for control. One of main mechanisms insects become resistant involves detoxification enzymes. this review, we illustrate some examples role major families enzymes such as cytochromes P450, carboxyl/cholinesterases, glutathione S-transferases (GST) transporters ATP-binding cassette (ABC) insecticide resistance. We compare available data four species, exigua, S. frugiperda, littoralis litura. Molecular underlying involvement these genes will be described, duplication CYP9A cluster, over-expression GST epsilon or point mutations acetylcholinesterase ABCC2. This review is not intended exhaustive but highlight key roles certain genes.

Language: Английский

Citations

106
Functional and Structural Diversity of Insect Glutathione S-transferases in Xenobiotic Adaptation DOI Creative Commons
Sonu Koirala B K, Timothy W. Moural, Fang Zhu

et al.

International Journal of Biological Sciences, Journal Year: 2022, Volume and Issue: 18(15), P. 5713 - 5723

Published: Jan. 1, 2022

As a superfamily of multifunctional enzymes that is mainly associated with xenobiotic adaptation, glutathione S-transferases (GSTs) facilitate insects' survival under chemical stresses in their environment.GSTs confer adaptation through direct metabolism or sequestration xenobiotics, and/or indirectly by providing protection against oxidative stress induced exposure.In this article, comprehensive overview current understanding on the versatile functions insect GSTs detoxifying compounds presented.The diverse structures different classes GSTs, specifically spatial localization and composition amino acid residues constituted active sites are also summarized.Recent availability whole genome sequences numerous species, accompanied RNA interference, X-ray crystallography, enzyme kinetics site-directed mutagenesis techniques have significantly enhanced our functional structural diversity GSTs.

Language: Английский

Citations

106
UDP-glycosyltransferases act as key determinants of host plant range in generalist and specialist Spodoptera species DOI Creative Commons
Huidong Wang,

Jing Song,

Benjamin J. Hunt

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(19)

Published: April 29, 2024

Phytophagous insects have evolved sophisticated detoxification systems to overcome the antiherbivore chemical defenses produced by many plants. However, how these biotransformation differ in generalist and specialist insect species their role determining host plant range remains an open question. Here, we show that UDP-glucosyltransferases (UGTs) play a key of within Spodoptera genus. Comparative genomic analyses breadth identified relatively conserved number UGT genes but high levels gene pseudogenization picta . CRISPR-Cas9 knockouts three main clusters frugiperda revealed UGT33 important allowing this utilize poaceous plants maize, wheat, rice, while UGT40 facilitate utilization cotton. Further functional vivo vitro SfUGT33F32 as mechanism allows S. detoxify benzoxazinoid DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one), potent insecticidal phytotoxin capacity is several species, , which specializes on Crinum plants, unable due nonfunctionalizing mutation SpUGT33F34 Collectively, findings provide insight into UGTs adaptation, mechanistic basis evolutionary transitions between generalism specialism offer molecular targets for controlling group notorious pests.

Language: Английский

Citations

16
A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance DOI Creative Commons
Fanghao Wan, Chuanlin Yin, Rui Tang

et al.

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Sept. 17, 2019

Abstract The codling moth Cydia pomonella , a major invasive pest of pome fruit, has spread around the globe in last half century. We generated chromosome-level scaffold assembly including Z chromosome and portion W chromosome. This reveals duplication an olfactory receptor gene ( OR3 ), which we demonstrate enhances ability C. to exploit kairomones pheromones locating both host plants mates. Genome-wide association studies contrasting insecticide-resistant susceptible strains identify hundreds single nucleotide polymorphisms (SNPs) potentially associated with insecticide resistance, three SNPs found promoter CYP6B2 . RNAi knockdown increases sensitivity two insecticides, deltamethrin azinphos methyl. high-quality genome informs genetic basis its invasiveness, suggesting distinctive capabilities adaptive potential that may explain worldwide expansion.

Language: Английский

Citations

140