Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 9, 2023

Threatening the global community is a wide variety of potential threats, most notably invasive pest species. Invasive species are non-native organisms that humans have either accidentally or intentionally spread to new regions. One effective and first lines control strategies for controlling pests application insecticides. These toxic chemicals employed get rid pests, but they pose great risks people, animals, plants. Pesticides heavily used in managing current era. Due overuse synthetic chemicals, numerous already developed resistance. The resistance development main reason failure manage Developing pesticide management techniques necessitates thorough understanding mechanisms through which insects acquire insecticide Insects use behavioral, biochemical, physiological, genetic, metabolic methods deal with can lead continuous overexpression detoxifying enzymes. An overabundance enzymes causes resistance, pesticides rendering them ineffective against pests. A key factor amplification certain enzymes, specifically esterases, Glutathione S-transferase, Cytochromes p450 monooxygenase, hydrolyses. Additionally, insect guts offer unique habitats microbial colonization, gut bacteria may serve their hosts useful services. Most importantly, detoxification insecticides leads development. complete knowledge could be very helpful coping challenges effectively developing Integrated Pest Management particularly at lowering risk chemical environmental contaminants resulting health issues, it also ways

Language: Английский

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Functional and Structural Diversity of Insect Glutathione S-transferases in Xenobiotic Adaptation

International Journal of Biological Sciences, Journal Year: 2022, Volume and Issue: 18(15), P. 5713 - 5723

Published: Jan. 1, 2022

As a superfamily of multifunctional enzymes that is mainly associated with xenobiotic adaptation, glutathione S-transferases (GSTs) facilitate insects' survival under chemical stresses in their environment.GSTs confer adaptation through direct metabolism or sequestration xenobiotics, and/or indirectly by providing protection against oxidative stress induced exposure.In this article, comprehensive overview current understanding on the versatile functions insect GSTs detoxifying compounds presented.The diverse structures different classes GSTs, specifically spatial localization and composition amino acid residues constituted active sites are also summarized.Recent availability whole genome sequences numerous species, accompanied RNA interference, X-ray crystallography, enzyme kinetics site-directed mutagenesis techniques have significantly enhanced our functional structural diversity GSTs.

Language: Английский

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Flavonoids as biopesticides – Systematic assessment of sources, structures, activities and environmental fate

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 824, P. 153781 - 153781

Published: Feb. 14, 2022

Language: Английский

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Divergent amplifications of CYP9A cytochrome P450 genes provide two noctuid pests with differential protection against xenobiotics

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(37)

Published: Sept. 5, 2023

Here, we provide mechanistic support for the involvement of CYP9A subfamily cytochrome P450 monooxygenases in detoxification host plant defense compounds and chemical insecticides Spodoptera exigua frugiperda . Our comparative genomics shows that a large cluster genes occurs two species but with significant differences its contents, including several species-specific duplicates substantial sequence divergence, both between orthologs duplicates. Bioassays CRISPR-Cas9 knockouts clusters show that, collectively, CYP9As can detoxify furanocoumarin (imperatorin xanthotoxin) representing three different chemotypes (pyrethroids, avermectins, oxadiazines). However, vitro metabolic assays heterologously expressed products individual particular activities against these compounds. We also find tight genetic linkage high levels pyrethroid resistance field strains species. propose their divergent amplifications have not only contributed to development broad ranges over long evolutionary timeframes supplied them diverse options evolving very recent past.

Language: Английский

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Identification of differentially expressed miRNAs associated with diamide detoxification pathways in Spodoptera frugiperda

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 21, 2024

The fall armyworm (FAW) Spodoptera frugiperda is a severe economic pest of multiple crops globally. Control this often achieved using insecticides; however, over time, S. has developed resistance to new mode action compounds, including diamides. Previous studies have indicated diamide complex developmental process involving detoxification genes. Still, the mechanism underlying possible involvement microRNAs in post-transcriptional regulation not yet been elucidated. In study, global screen (miRNAs) revealed 109 known and 63 novel miRNAs. Nine miRNAs (four five novel) were differentially expressed between insecticide-resistant -susceptible strains. Gene Ontology analysis predicted putative target transcripts encoding significant genes belonging pathways. Additionally, are involved response exposure, indicating they probably associated with pathway. Thus, study provides comprehensive evidence for link repressed miRNA expression induced that possibly mediate through regulation. These findings highlight important clues further research unravel roles mechanisms conferring resistance.

Language: Английский

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Differential detoxification enzyme profiles in C-corn strain and R-rice strain of Spodoptera frugiperda by comparative genomic analysis: insights into host adaptation

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 6, 2025

The fall armyworm (FAW) Spodoptera frugiperda, a highly invasive, polyphagous pest, poses global agricultural threat. It has two strains, the C-corn and R-rice each with distinct host preferences. This study compares detoxification enzyme gene families across these strains related species to explore their adaptation diverse plant metabolites. A total of 1,995 detoxification-related genes, including cytochrome P450 monooxygenases (CYPs), carboxylesterases (COEs), glutathione S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), ATP-binding cassette transporters (ABCs), were identified genus Spodoptera, S. littoralis, litura, picta, exigua, both FAW strains. higher abundance phase I enzymes (CYPs COEs) GSTs was observed in species, while exhibited fewer notable differences copy numbers between C R Analyses at subfamily level revealed significant variation distribution expression, particularly within II enzymes. Expansions CYP6AE detected strain, contractions GST-ε, CYP9A, CYP4M, UGT33B, UGT33F occurred In contrast, no substantial III ABC Functional predictions protein interaction networks suggest broader expansion metabolism-related genes strain compared strain. These findings emphasize pivotal role adaptation, providing molecular insights into FAW's capacity for range expansion, which are crucial devising targeted sustainable pest management strategies. differential expression different frugiperda.

Language: Английский

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Unveiling insecticidal compounds from the invasive weed Tithonia diversifolia against Spodoptera litura and elucidating mechanisms via transcriptomic analysis

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 210, P. 106354 - 106354

Published: March 1, 2025

Language: Английский

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Monitoring Resistance and Biochemical Studies of Three Egyptian Field Strains of Spodoptera littoralis (Lepidoptera: Noctuidae) to Six Insecticides

Toxics, Journal Year: 2023, Volume and Issue: 11(3), P. 211 - 211

Published: Feb. 24, 2023

Spodoptera littoralis (Boisd.) is a prominent agricultural insect pest that has developed resistance to variety of insecticide classes. In this study, the three field strains S. littoralis, collected over consecutive seasons (2018 2020) from Egyptian Governorates (El-Fayoum, Behera and Kafr El-Shiekh), six insecticides was monitored.Laboratory bioassays were carried out using leaf-dipping method examine susceptibility laboratory tested insecticides. Activities detoxification enzymes determined in an attempt identify mechanisms.The results showed LC50 values ranged 0.0089 132.24 mg/L, corresponding ratio (RR) 0.17 4.13-fold compared with susceptible strain. Notably, low spinosad all strains, very alpha-cypermethrin chlorpyrifos. On other hand, no methomyl, hexaflumeron or Bacillus thuringiensis. The determination enzymes, including carboxylesterases (α- β-esterase), mixed function oxidase (MFO) glutathione-S-transferase (GST), target site acetylcholinesterase (AChE), revealed had significantly different activity levels strain.Our findings, along tactics, are expected help management Egypt.

Language: Английский

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The global trend of nanomaterial usage to control the important agricultural arthropod pests: A comprehensive review

Plant Stress, Journal Year: 2023, Volume and Issue: 10, P. 100208 - 100208

Published: Aug. 26, 2023

Global warming and climate change have favored the resurgence of arthropod pests their short lifecycle. The massive use synthetic chemicals for insect pest control has indirectly global warming, ecotoxicity, insecticide resistance in agricultural pests. Additionally, increasing population world required more food, a significant proportion produced is deteriorated by other biotic abiotic factors. Recently, nanotechnology revolutionized industries current era. Extremely small size physio-morphic properties nanomaterials attracted interest researchers to develop nano-fertilizers, nano-pesticides, nano-herbicides that overwhelmed aforementioned problems increase crop productivity. Micronutrient based nano-pesticides like Ag, ZnO, TiO2, Cu, SiO2 not only enhanced pest's biogenicity but also boost-up There are some apprehensions regarding nanomaterial synthesis usage as characteristics nanostructured metals offers cheap excellent solution control. This review article provides comprehensive overview trend controlling important A bibliometric analysis was conducted evaluate research landscape identify key trends this field. encompasses various aspects, including emergence chemical pesticides, fate pesticides management, detrimental effects on ecosystem. role agroecosystems discussed, specifically focusing utilization management. an in-depth silver, zinc, copper, titanium, gold, iron, silica, aluminum nanoparticles control, highlighting efficacy mechanisms action. findings underscore importance continued responsible implementation overcome limitations harness full potential management benefit sustainable agriculture.

Language: Английский

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Variation in egg mass scale thickness of three Spodoptera species and its effects on egg parasitoid performance

Journal of Pest Science, Journal Year: 2023, Volume and Issue: 96(4), P. 1393 - 1402

Published: Feb. 28, 2023

Language: Английский

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