A Conserved Regulatory Circuit Controls Large Adhesins in Vibrio cholerae DOI Creative Commons
Giordan Kitts,

Krista M. Giglio,

David Zamorano‐Sánchez

et al.

mBio, Journal Year: 2019, Volume and Issue: 10(6)

Published: Dec. 2, 2019

The dinucleotide second messenger c-di-GMP has emerged as a central regulator of reversible cell attachment during bacterial biofilm formation. A prominent adhesion mechanism first identified in pseudomonads combines two c-di-GMP-mediated processes: transcription large adhesin and its surface display via posttranslational proteolytic control. Here, we characterize an orthologous effector system show that it is operational Vibrio cholerae, where regulates distinct classes adhesins. Through structural analyses, reveal conserved autoinhibition the receptor controls proteolysis present structure c-di-GMP-bound module. We further establish functionality periplasmic protease controlled by against Finally, functional assays identify physiological roles both c-di-GMP-regulated adhesins Together, our studies highlight conservation highly efficient signaling circuit for control expression versatility revealing strain-specific variations.IMPORTANCEVibrio causative agent diarrheal disease cholera, benefits from sessile lifestyle enhances survival outside host but also contributes to colonization infectivity. been formation, including V. cholerae; however, understanding pathways contribute this process incomplete. define stability proteins at which are important Insight into regulatory underlying formation may inform targeted strategies interfere with renders bacterium remarkably adaptable changing environments.

Language: Английский

Biofilms: hot spots of horizontal gene transfer (HGT) in aquatic environments, with a focus on a new HGT mechanism DOI Creative Commons
Kimihiro Abe, Nobuhiko Nomura, Satoru Suzuki

et al.

FEMS Microbiology Ecology, Journal Year: 2020, Volume and Issue: 96(5)

Published: Feb. 27, 2020

Biofilms in water environments are thought to be hot spots for horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs). ARGs can spread via HGT, though mechanisms known and have been shown depend on the environment, bacterial communities mobile genetic elements. Classically, HGT include conjugation, transformation transduction; more recently, membrane vesicles (MVs) reported as DNA reservoirs implicated interspecies HGT. Here, we review current knowledge with a focus role MVs methodological innovations research.

Language: Английский

Citations

311
Mechanomicrobiology: how bacteria sense and respond to forces DOI
Yves F. Dufrêne, Alexandre Persat

Nature Reviews Microbiology, Journal Year: 2020, Volume and Issue: 18(4), P. 227 - 240

Published: Jan. 20, 2020

Language: Английский

Citations

247
Mechanisms of DNA Uptake by Naturally Competent Bacteria DOI
David Dubnau, Melanie Blokesch

Annual Review of Genetics, Journal Year: 2019, Volume and Issue: 53(1), P. 217 - 237

Published: Aug. 21, 2019

Transformation is a widespread mechanism of horizontal gene transfer in bacteria. DNA uptake to the periplasmic compartment requires DNA-uptake pilus and DNA-binding protein ComEA. In gram-negative bacteria, first pulled toward outer membrane by retraction then taken up binding ComEA, acting as Brownian ratchet prevent backward diffusion. A similar probably operates gram-positive bacteria well, but these systems have been less well characterized. Transport, defined movement single strand transforming cytosol, channel ComEC. Although understood about this process, it may be driven proton symport. review we also describe various phenomena that are coordinated with expression competence for transformation, such fratricide, kin-discriminatory killing neighboring cells, competence-mediated growth arrest.

Language: Английский

Citations

174
Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae DOI Creative Commons
Lucia Vidakovic, Sofya Mikhaleva, Hannah Jeckel

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(12), P. 2690 - 2704.e20

Published: June 1, 2023

Biofilm formation is generally recognized as a bacterial defense mechanism against environmental threats, including antibiotics, bacteriophages, and leukocytes of the human immune system. Here, we show that for pathogen Vibrio cholerae, biofilm not only protective trait but also an aggressive to collectively predate different cells. We find V. cholerae forms biofilms on eukaryotic cell surface using extracellular matrix comprising primarily mannose-sensitive hemagglutinin pili, toxin-coregulated secreted colonization factor TcpF, which differs from composition other surfaces. These encase cells establish high local concentration hemolysin kill before disperse in c-di-GMP-dependent manner. Together, these results uncover how bacteria employ multicellular strategy invert typical relationship between hunters hunted.

Language: Английский

Citations

47
Surface Sensing and Adaptation in Bacteria DOI Creative Commons
Benoît‐Joseph Laventie, Urs Jenal

Annual Review of Microbiology, Journal Year: 2020, Volume and Issue: 74(1), P. 735 - 760

Published: Sept. 8, 2020

Bacteria thrive both in liquids and attached to surfaces. The concentration of bacteria on surfaces is generally much higher than the surrounding environment, offering ample opportunity for mutualistic, symbiotic, pathogenic interactions. To efficiently populate surfaces, they have evolved mechanisms sense mechanical or chemical cues upon contact with solid substrata. This particular importance pathogens that interact host tissue In this review we discuss how are able use information adapt their physiology behavior new environment. We first survey mechanosensing chemosensing outline specific macromolecular structures can inform about then converted biochemical signals activate cellular processes a defined chronological order describe role two key second messengers, c-di-GMP cAMP, process.

Language: Английский

Citations

89
Roadmap on emerging concepts in the physical biology of bacterial biofilms: from surface sensing to community formation DOI Creative Commons
Gerard C. L. Wong, Jyot D. Antani, Pushkar P. Lele

et al.

Physical Biology, Journal Year: 2021, Volume and Issue: 18(5), P. 051501 - 051501

Published: Jan. 19, 2021

Bacterial biofilms are communities of bacteria that exist as aggregates can adhere to surfaces or be free-standing. This complex, social mode cellular organization is fundamental the physiology microbes and often exhibits surprising behavior. more than sum their parts: single-cell behavior has a complex relation collective community behavior, in manner perhaps cognate between atomic physics condensed matter physics. Biofilm microbiology relatively young field by biology standards, but it already attracted intense attention from physicists. Sometimes, this takes form seeing inspiration for new In roadmap, we highlight work those who have taken opposite strategy: physicists physical scientists use engage concepts bacterial biofilm microbiology, including adhesion, sensing, motility, signaling, memory, energy flow, formation cooperativity. These contributions juxtaposed with microbiologists made recent important discoveries on using state-of-the-art methods. The roadmap exemplify how well combined achieve synthesis, rather just division labor.

Language: Английский

Citations

72
Bacteria autoaggregation: how and why bacteria stick together DOI Creative Commons
El-shama Q. Nwoko, Iruka N. Okeke

Biochemical Society Transactions, Journal Year: 2021, Volume and Issue: 49(3), P. 1147 - 1157

Published: June 10, 2021

Autoaggregation, adherence between identical bacterial cells, is important for colonization, kin and kind recognition, survival of bacteria. It directly mediated by specific interactions proteins or organelles on the surfaces interacting cells indirectly presence secreted macromolecules such as eDNA exopolysaccharides. Some autoaggregation effectors are self-associating present interesting paradigms protein interaction. Autoaggregation can be beneficial deleterious at times niches. is, therefore, typically regulated through transcriptional post-transcriptional mechanisms epigenetically phase variation. contribute to adherence, biofilm formation other higher-level functions. However, only required these phenotypes in some Thus, should detected, studied measured independently using both qualitative quantitative vitro ex vivo methods. If better understood, holds potential discovery new therapeutic targets that could cost-effectively exploited.

Language: Английский

Citations

67
Type IV Pili: dynamic bacterial nanomachines DOI
Courtney K. Ellison, Gregory B. Whitfield, Yves V. Brun

et al.

FEMS Microbiology Reviews, Journal Year: 2021, Volume and Issue: 46(2)

Published: Nov. 8, 2021

Bacteria and archaea rely on appendages called type IV pili (T4P) to participate in diverse behaviors including surface sensing, biofilm formation, virulence, protein secretion motility across surfaces. T4P are broadly distributed fibers that dynamically extend retract, this dynamic activity is essential for their function broad processes. Despite the essentiality of dynamics function, little known about role these molecular mechanisms controlling them. Recent advances microscopy have yielded insight into functions recent structural work has expanded what inner workings motor. This review discusses progress understanding regulation, dynamics.

Language: Английский

Citations

66
Mechanisms Underlying Vibrio cholerae Biofilm Formation and Dispersion DOI

Jennifer K. Teschler,

Carey D. Nadell, Knut Drescher

et al.

Annual Review of Microbiology, Journal Year: 2022, Volume and Issue: 76(1), P. 503 - 532

Published: June 7, 2022

Biofilms are a widely observed growth mode in which microbial communities spatially structured and embedded polymeric extracellular matrix. Here, we focus on the model bacterium Vibrio cholerae summarize current understanding of biofilm formation, including initial attachment, matrix components, community dynamics, social interactions, molecular regulation, dispersal. The regulatory network that orchestrates decision to form disperse from biofilms coordinates various environmental inputs. These cues integrated by several transcription factors, RNAs, second-messenger molecules, bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP). Through complex mechanisms, V. weighs energetic cost forming against benefits protection interaction provide.

Language: Английский

Citations

66
Protein nanowires with tunable functionality and programmable self-assembly using sequence-controlled synthesis DOI Creative Commons
Daniel Mark Shapiro, Gunasheil Mandava, Sibel Ebru Yalcin

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Feb. 11, 2022

Abstract Advances in synthetic biology permit the genetic encoding of chemistries at monomeric precision, enabling synthesis programmable proteins with tunable properties. Bacterial pili serve as an attractive biomaterial for development engineered protein materials due to their ability self-assemble into mechanically robust filaments. However, most biomaterials lack electronic functionality and atomic structures putative conductive are not known. Here, we engineer high conductivity produced by a genomically-recoded E. coli strain. Incorporation tryptophan increased individual filaments >80-fold. Computationally-guided ordering nanostructures 5-fold compared unordered networks. Site-specific conjugation gold nanoparticles, facilitated incorporating nonstandard amino acid propargyloxy-phenylalanine, filament ~170-fold. This work demonstrates sequence-defined production highly-conductive nanowires hybrid organic-inorganic genetically-programmable functionalities accessible nature or through chemical-based synthesis.

Language: Английский

Citations

52